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Bacillus sp. SMMJ-2의 Keratinolytic protease생산최적조건

Bacillus sp. SMMJ-2, keratinolytic protease,feather-degrading

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주제분류생물학

초록

스웨덴 스톡홀름의 항구부근 토양시료로부터 분리한Bacillus sp. SMMJ-2는 단백질 분해활성이 높은 균주로써 특히 keratinolytic protease 생산성이 우수한 균주이다.효소생산을 위한 최적 배지조성은 0.7% K2HPO4, 0.2%KH2PO4, 1.0% fructose, 1.2% soybean meal(roasted),그리고 0.01% Na2CO3 이었으며 배양초기 pH는 7.0, 배양온도는 30oC에서 200 rpm 조건일 때 효소생산이 가장우수하였으며 이때의 활성은 105 units/ml/min로 조사되었다.감사의 글본 연구는 산업자원부의 지역혁신 인력양성사업의 연구결과로 수행되었으므로 이에 감사드립니다.

참고문헌 (26)

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