황련 추출물의 고추역병에 대한 In-vitro항진균 활성 및 고추 생육촉진 효과
Bioactivity of the Extract of Coptis chinensis: In-vitro Antifungal Activity against Phytophthora capsici and Growth-promotion Effect in Red-pepper.
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Abstract
To investigate antiphytopathogenic fungal activity of Coptis chinensis, the methanol extract and its organic solvent fractions were prepared. Using the extract and the fractions, in-vitro spore-germination inhibition and mycelial-growth inhibition activities were evaluated against Colletotrichum gloeosporioides, Phytohpthora capsici, Pyricularia grisea, Rhizoctonia solani, Botryosphaeri dothidea, Glomerella cingulata, respectively. Treatment of the methanol extract (500 mg/mL) into the spore of phytopathogenic fungi completely inhibited germinations for 5 days, except B. dothidea, and showed strong antifungal activities against P. grisea and B. cinerea, and antioomycetes activity against P. capsici. The minimal growth inhibition concentrations of the methanol extract against P. grisea, B. cinerea and P. capsici were 300, 300, and 500 mg/mL, respectively. For practical application of C. chinensis in red-pepper field, the hot-water extract (1,000 mg/mL) was prepared in commercial facility, after evaluation of heat stability and solvent-extraction yields of antifungal substances. The 3- times leaf-spray of the extract from June to August, 2008 did not show any deleterious effect to red-pepper. In fact, the leaf-spray promoted plant growth including leaf, root and fruit. The average weight and rind of each fruit were increased to 119% and 117% comparison to those of without treatments. Our results suggest that C. chinensis is a useful source for control of red-pepper diseases and plant growth.
참고문헌 (25)
- 안봉전, "黃連의 생리활성검증 및 화장품 소재로서의 이용가능성", 대한본초학회지, 20(3), pp.83~92 (2005), 대한본초학회
- 배지현, "식중독 유발세균의 증식에 미치는 황련 추출물의 효과", 한국식품영양학회지, 18(1), pp.81~87 (2005), 한국식품영양학회
- CHOI, GYUNG JA, "In Vivo Antifungal Activities of 67 Plant Fruit Extracts Against Six PlantPathogenic Fungi", Journal of Microbiology and Biotechnology, 16(3), pp.491~495 (2006), 한국미생물·생명공학회
- Chung, I. M, "Separation and activity test of antifungal substance from C. japonica extract", Anal. Sci. Technol, 10, pp.153~159 (1997)
- Hwang T. M, "Berberine induces apoptosis through a mitochondria caspases pathway in human hepatoma cells", Arch. Toxicol., 80, pp.62~73 (2006)
- 정현아, "Inhibitory Activities of the Alkaloids from Coptidis Rhizoma against Aldose Reductase", Archives of Pharmacal Research, 31(11), pp.1405~1412 (2008), 대한약학회
- 박용기, "황련 추출물의 분획화 및 BV2 microglial cells에서 LPS에 의해 유도되는 nitric oxide 생성억제효과 검정", 대한본초학회지, 22(2), pp.73~78 (2007), 대한본초학회
- Keumann V, "In vitro cytotoxicity of berberine against HeLa and L1210 cancer cell lines.Pharmazie 59:548-551", Pharmazie, 59, pp.548~551 (2004)
- 김일출, "갈근, 복령 및 황련의 항산화성 및 미백효과", 韓國油化學會誌, 25(2), pp.219~225 (2008), 한국유화학회
- 김재영, "수종의 한약재 추출물의 항산화 활성 및 항진균 활성", 한국미생물·생명공학회지, 37(1), pp.42~48 (2009), 한국미생물·생명공학회
- LEE, CHI-HOON, "In Vivo Antifungal Effects of Coptis japonica Root-Derived Isoquinoline Alkaloids Against Phytopathogenic Fungi", Journal of Microbiology and Biotechnology, 15(6), pp.1402~1407 (2005), 한국미생물·생명공학회
- 이중복, "고추역병 유발병원균 Phytophthora capsici에 대한 Bacillus sp. AM-651의 항진균활성", 한국미생물·생명공학회지, 36(3), pp.227~232 (2008), 한국미생물·생명공학회
- Lee, J. Y, "In vitro antimicrobial and in vivo antioomycete activities of the novel antibiotic thiobutacin", Pest Manag. Sci., 64, pp.172~177 (2008)
- Liu, Z, "Berberine induces p53-dependent cell cycle arrest and apoptosis of human osteosarcoma cells by inflicting DNA damage", Mutat. Res, 662, pp.75~83 (2009)
- Ma, C.Y, "Growth inhibition and induction of apoptosis in U937 cells by Coptis chinesis extract", J. Food Sci., 73, pp.H127~H133 (2008)
- 민병선, "Quantitative Determination of protoberebrines from the Roots of Coptis chinensis", Natural Product Sciences, 14(1), pp.68~72 (2008), 한국생약학회
- 박용기, "黃連의 주성분인 Berberine의 뇌신경소교세포로부터 LPS에 의해 유도되는 염증매개물질 생성억제효과", 대한본초학회지, 22(4), pp.117~125 (2007), 대한본초학회
- 배경화, "안동소주 발효액으로부터 분리한 젖산 세균의 동정 및 발효 특성", 한국미생물·생명공학회지, 35(4), pp.310~315 (2007), 한국미생물·생명공학회
- 손호용, "호이초(Saxifraga stolonifera) 지상부의 항균, 항혈전 및 항산화 활성 평가", 한국미생물·생명공학회지, 36(3), pp.195~200 (2008), 한국미생물·생명공학회
- Sohn, H. Y, "Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent", Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine, 11, pp.666~672 (2004)
- Tanabe, H, "Selective inhibition of vascular smooth muscle cell proliferation by coptisine isolated from Coptis rhizome, one of the crude drugs composing Kampo medicines Unsei-in", Phytomedicine, 13, pp.334~342 (2006)
- Tang, L. Q, "Effects of berberine on diabetes induced by allxan and a high-fat", high-cholesterol diet in rats, 2006, pp.108, 109~115
- Yan, D, "Antimicrobial properties of berberine alkaloids in Coptis chinensis Franch by microcalorimetry", J. Biochem. Biophys. Methods, 70, pp.845~849 (2008)
- Yoo, J. K, "Fungicidal activity of oriental medicinal plant extracts against plant pathogenic fungi", Agric. Chem. Biotechnol, 41, pp.600~604 (1998)
- Yong-Ouk You, "Antimicrobial Activity of Berberine Alone and in Combination with Ampicillin or Oxacillin Against Methicillin-Resistant Staphylococcus aureus", Journal of Medicinal Food, 8(4), pp.454~461 (2005), 한국식품영양과학회