Alginate Lyase 생산 균주 Pseudomonas sp. N7151-6의 분리 및 특성
Identification and Characterization of Alginate Lyase Producing Pseudomonas sp. N7151-6
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A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be 30℃ and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and 30℃, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of 23-37℃. The total activity of alginate lyase produced was reached about 110 unit/L.
Abstract
A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be 30℃ and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and 30℃, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of 23-37℃. The total activity of alginate lyase produced was reached about 110 unit/L.
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