동결건조 보호제와 기질이 동결건조된 Bacillus sp. SH1RP8의 생존율에 미치는 영향
Effect of Substrates and Lyoprotectant on the Survival Ratio of Lyophilized Bacillus sp. SH1RP8
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본 연구는 식물성장촉진 근권세균인 Bacillus sp. SH1RP8를 친환경 생물비료로 이용하기 위하여 수행되었다. SH1RP8 균주를 동결건조시 세포의 용혈을 방지하도록 여러 가지 동결건조 보호제를 첨가하여 균주의 성장과 활성에 미치는 영향을 알아보았다. SH1RP8 균주의 동결건조 시 동결건조 보호제로 skim milk, glucose, peptone 등을 이용하였을 때, 그중 5%의 skim milk를 첨가하였을 때 가장 높은 (30.6%)의 생존율을 보였다. 또한, 균주의 성장을 촉진하는 기질 그룹을 첨가하여 5%의 skim milk 단독으로 첨가한 경우와 기질그룹을 각각 첨가한 경우의 동결건조 보호 효과를 비교하여 보았다. 그 결과 5% skim milk에 glycerol을 동시에 첨가할 경우 균주의 생존율이 skim milk 단독 첨가효과와 비교 시 214.29%의 향상율을 보여주었다. 또한 동결건조된 Bacillus sp. SH1RP8 은 매우 효과적인 PGPR로 활성을 보여주어 생물비료로서의 훌륭한 기능이 기대된다.
Abstract
In order to develop an eco-friendly biofertilizer, a plant growth promoting rhizobacterium (PGPR), Bacillus sp., was investigated. SH1RP8 was lyophilized via freeze-drying along with other protective agents that protect cells from lysis. The freezedried powder of Bacillus sp. SH1RP8, containing 5% skim milk (w/v), exhibited the highest survival rate of 30.6% among all the protective agents (skim milk, glucose, and peptone).The lyoprotective effect of the skim milk, mixture including 5% skim milk, and substrates on the survival of the test strain was examined. Control group was added only skim milk and test groups were added skim milk and other substrates. As a result, the group supplemented with both glycerol and 5% skim milk showed the protective effect much higher by 114.29% than the control group. Freeze-dried Bacillus sp. SH1RP8 could be a good candidate as a potential biofertilizer due to its effective PGPR activity.
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