CO 내성을 갖는 Clostridium sp. AWRP 돌연변이주의 분리 및 이를 이용한 합성가스로부터의 에탄올 생산 연구
Enhanced Alcohol Production from Synthesis Gas Using a CO-resistant Mutant of Clostridium sp. AWRP
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In this study, the carbon monoxide (CO)-fermenting acetogen, Clostridium sp. AWRP was subjected to chemical mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to generate a CO-resistant mutant. Among the 26 colonies obtained, the highest alcohol production was observed in one isolate, named C1. Compared to the wild-type strain, the C1 strain exhibited 1.5- and 3.4-fold higher CO consumption rate and alcohol selectivity, respectively. The total CO consumption of strain C1 could be further enhanced by increasing the content of metal ions, such as nickel and iron. The highest ethanol titer (5.7 g/l) was achieved by 5-fold increase in the iron concentration.
Abstract
In this study, the carbon monoxide (CO)-fermenting acetogen, Clostridium sp. AWRP was subjected to chemical mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to generate a CO-resistant mutant. Among the 26 colonies obtained, the highest alcohol production was observed in one isolate, named C1. Compared to the wild-type strain, the C1 strain exhibited 1.5- and 3.4-fold higher CO consumption rate and alcohol selectivity, respectively. The total CO consumption of strain C1 could be further enhanced by increasing the content of metal ions, such as nickel and iron. The highest ethanol titer (5.7 g/l) was achieved by 5-fold increase in the iron concentration.
참고문헌 (21)
- Lee Y, "Systems metabolic engineering strategies for non-natural microbial polyester production", Biotechnol. J, 14, pp.e1800426~ (2019)
- Liao JC, "Fuelling the future: microbial engineering for the production of sustainable biofuels", Nat. Rev. Microbiol, 14, pp.288~304 (2016)
- Bengelsdorf FR, "Bacterial synthesis gas (syngas) fermentation", Environ. Technol, 34, pp.1639~1651 (2013)
- Ragsdale SW, "Acetogenesis and the Wood-Ljungdahl pathway of CO2 fixation", Biochim. Biophys. Acta, 1784, pp.1873~1898 (2008)
- Bengelsdorf FR, "Bacterial anaerobic synthesis gas (Syngas) and CO2+H2 fermentation", Adv. Appl. Microbiol, 103, pp.143~221 (2018)
- iew F, "Metabolic engineering of Clostridium autoethanogenum for selective alcohol production", Metab. Eng, 40, pp.104~114 (2017)
- Jones SW, "CO2 fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion", Nat. Commun, 7, pp.12800~ (2016)
- Park S, "Acetate-assisted increase of butyrate production by Eubacterium limosum KIST612 during carbon monoxide fermentation", Bioresour. Technol, 245, pp.560~566 (2017)
- Bengelsdorf FR, "Industrial acetogenic biocatalysts: a comparative metabolic and genomic analysis", Front Microbiol, 7, pp.1036~ (2016)
- Mayer A, "Carbon monoxide conversion with Clostridium aceticum", Biotechnol. Bioeng, 115, pp.2740~2750 (2018)
- -hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO, "J. Bacteriol", 2013, 4373~4386
- Lee J, "Domestication of the novel alcohologenic acetogen Clostridium sp. AWRP: from isolation to characterization for syngas fermentation", Biotechnol. Biofuels, 12, pp.228~ (2019)
- Wolin EA, "Formation of methane by bacterial extracts", J. Biol. Chem, 238, pp.2882~2886 (1963)
- Connor MR, "3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and twophase fermentation", Appl. Microbiol. Biotechnol, 86, pp.1155~1164 (2010)
- Kim MS, "COdependent H2 production by genetically engineered Thermococcus onnurineus NA1", Appl. Environ. Microbiol, 79, pp.2048~2053 (2013)
- Tanner RS, "Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I", Int. J. Syst. Bacteriol, 43, pp.232~236 (1993)
- Ragsdale SW, "Enzymology of the Wood-Ljungdahl pathway of acetogenesis", Ann. N Y Acad. Sci, 1125, pp.129~136 (2008)
- Guo Y, "Medium optimization for ethanol production with Clostridium autoethanogenum with carbon monoxide as sole carbon source", Bioresour. Technol, 101, pp.8784~8789 (2010)
- Saxena J, "Effect of trace metals on ethanol production from synthesis gas by the ethanologenic acetogen, Clostridium ragsdalei", J. Ind. Microbiol. Biotechnol, 38, pp.513~521 (2011)
- Abubackar HN, "Carbon monoxide fermentation to ethanol by Clostridium autoethanogenum in a bioreactor with no accumulation of acetic acid. Bioresour", Technol, 186, pp.122~127 (2015)
- Mukund S, "The novel tungsten-iron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase. Evidence for its participation in a unique glycolytic pathway", J. Biol. Chem, 266, pp.14208~14216 (1991)