합성가스 발효에서 배지 내 Vitamin 농도의 에탄올 생산에 대한 영향
Effect of Culture Medium Vitamin Concentration of Culture Medium on Ethanol Production in Syngas Fermentation
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In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 μg/L were used in the culture experiments at 0.2, 2, 20, and 100 μg/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 μg/L biotin and folic acid, respectively. Moreover, Thiaminethiamine-–HCl at concentrations of 0.5, 5, 50, and 250 μg/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 μg/L of thiamine-–HCl. As a resultThus, the optimized concentrations of biotin, thiamine-–HCl, and folic acid were determined at 0.2, 0.5, and 0.2 μg/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.
Abstract
In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 μg/L were used in the culture experiments at 0.2, 2, 20, and 100 μg/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 μg/L biotin and folic acid, respectively. Moreover, Thiaminethiamine-–HCl at concentrations of 0.5, 5, 50, and 250 μg/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 μg/L of thiamine-–HCl. As a resultThus, the optimized concentrations of biotin, thiamine-–HCl, and folic acid were determined at 0.2, 0.5, and 0.2 μg/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.
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