Effects of elevated CO₂ on organic matter decomposition capacities and community structure of sulfate-reducing bacteria in salt marsh sediment
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Increasing atmospheric CO₂ affects the soil carbon cycle by influencing microbial activity and the carbon pool. In this study, the effects of elevated CO₂ on extracellular enzyme activities (EEA; β-glucosidase, N-acetylglucosaminidase,aminopeptidase) in salt marsh sediment vegetated with Suaeda japonica were assessed under ambient atmospheric CO₂ concentration (380 ppm) or elevated CO₂ concentration (760 ppm) conditions. Additionally, the community structure of sulfate-reducing bacteria (SRB) was analyzed via terminal restriction fragments length polymorphism (T-RFLP). Sediment with S. japonica samples were collected from the Hwangsando intertidal flat in May 2005, and placed in small pots (diameter 6 cm, height 10 cm). The pots were incubated for 60 days in a growth chamber under two different CO₂concentration conditions. Sediment samples for all measurements were subdivided into two parts: surface (0-2 cm)and rhizome (4-6 cm) soils. No significant differences were detected in EEA with different CO₂ treatments in the surface and rhizome soils. However, the ratio of β-glucosidase activity to N-acetylglucosaminidase activity in rhizome soil was significantly lower (P < 0.01) at 760 ppm CO₂ than at 380 ppm CO₂, thereby suggesting that the contribution of fungi to the decomposition of soil organic matter might in some cases prove larger than that of bacteria. Community structures of SRB were separated according to different CO₂ treatments, suggesting that elevated CO₂ may affect the carbon and sulfur cycle in salt marshes.
Abstract
Increasing atmospheric CO₂ affects the soil carbon cycle by influencing microbial activity and the carbon pool. In this study, the effects of elevated CO₂ on extracellular enzyme activities (EEA; β-glucosidase, N-acetylglucosaminidase,aminopeptidase) in salt marsh sediment vegetated with Suaeda japonica were assessed under ambient atmospheric CO₂ concentration (380 ppm) or elevated CO₂ concentration (760 ppm) conditions. Additionally, the community structure of sulfate-reducing bacteria (SRB) was analyzed via terminal restriction fragments length polymorphism (T-RFLP). Sediment with S. japonica samples were collected from the Hwangsando intertidal flat in May 2005, and placed in small pots (diameter 6 cm, height 10 cm). The pots were incubated for 60 days in a growth chamber under two different CO₂concentration conditions. Sediment samples for all measurements were subdivided into two parts: surface (0-2 cm)and rhizome (4-6 cm) soils. No significant differences were detected in EEA with different CO₂ treatments in the surface and rhizome soils. However, the ratio of β-glucosidase activity to N-acetylglucosaminidase activity in rhizome soil was significantly lower (P < 0.01) at 760 ppm CO₂ than at 380 ppm CO₂, thereby suggesting that the contribution of fungi to the decomposition of soil organic matter might in some cases prove larger than that of bacteria. Community structures of SRB were separated according to different CO₂ treatments, suggesting that elevated CO₂ may affect the carbon and sulfur cycle in salt marshes.
참고문헌 (49)
- Arp WJ, "Interactions between C3 and C4 salt marsh plant species during four years of exposure to elevated atmospheric CO2", Vegetatio, 104, pp.133~143 (1993)
- Azam F, "Microbial control of oceanic carbon flux: the plot thickens", Science, 280, pp.694~696 (1998)
- Carney KM, "Altered soil microbial community at elevated CO2 leads to loss of soil carbon.Proc Natl Acad Sci USA 104:4990-4995", Proc Natl Acad Sci USA, 통권 : 104, pp.4990~4995 (2007)
- Cheng W, "Elevated CO2, rhizosphere processes, and soil organic matter decomposition", Plant Soil, 202, pp.167~174 (1998)
- Chung H, "Plant species richness, elevated CO2, and atmospheric nitrogen deposition alter soil microbial community composition and function", Global Change Biol, 13, pp.980~989 (2007)
- Cotrufo MF, "Elevated CO2 reduces field decomposition rates of Betula pendula (Roth) leaf litter", Oecologia, 106, pp.525~530 (1996)
- Curtis PS, "Elevated atmospheric CO2 effects on belowground processes in C3 and C4 estuarine marsh communities", Ecology, 71, pp.2001~2006 (1990)
- DeLucia EH, "Net primary production of a forest ecosystem with experimental CO2 enrichment", Science, 284, pp.1177~1179 (1999)
- Detwiler RP, "Tropical forests and the global carbon cycle", Science, 239, pp.42~47 (1988)
- Dhillion SS, "Assessing the impact of elevated CO2 on soil microbial activity in a Mediterranean model ecosystem", Plant Soil, 187, pp.333~342 (1996)
- Domanski G, "Carbon flows in the rhizosphere of ryegrass (Lolium perenne)", J Plant Nutr Soil Sci, 164, pp.381~387 (2001)
- Drake BG, "Effects of Elevated Carbon Dioxide on Chesapeake Bay Wetlands. IV. Ecosystem and Whole Plant Responses April-November, 1988. US DOE", CO2 Greenbook 051- Atmospheric and Climate Research Division, ER-76, US Department of Energy, Washington, DC, 1988
- Drissner D, "Nine years of enriched CO2 changes the function and structural diversity of soil microorganisms in a grassland", Eur J Soil Sci, 58, pp.260~269 (2007)
- Duarte CM, "The fate of marine autotrophic production", Limnol Oceanogr, 41통권 : 1758, pp.1766~ (1996)
- Duarte CM, "Major role of marine vegetation on the oceanic carbon cycle", Biogeosciences, 2, pp.1~8 (2005)
- Erickson JE, "Salinity and sea level mediate elevated CO2 effects on C3-C4 plant interactions and tissue nitrogen in a Chesapeake Bay tidal wetland", Global Change Biol, 13, pp.202~215 (2007)
- Finzi AC, "Microbial community responses to atmospheric carbon dioxide enrichment in a warm-temperate forest", Ecosystems, 9, pp.215~226 (2006)
- Freeman C, "Export of dissolved organic carbon from peatlands under elevated carbon dioxide levels", Nature, 430, pp.195~198 (2004)
- Goldhaber MB, "Controls and consequences of sulfate reduction rates in recent marine sediments", Soil Sci, 119, pp.42~55 (1975)
- Gray AJ, "Climate impacts on pioneer salt-marsh plants", Clim Res, 18, pp.105~112 (2001)
- Hammel KE, "Fungal degradation of lignin. In: Driven by Nature: Plant Litter Quality and Decomposition" (1997), CAB International
- Hirschel G, "Will rising atmospheric CO2 affect leaf litter quality and in situ decomposition rates in native plant communities?", Oecologia, 110, pp.387~392 (1997)
- Hoppe HG, "Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates", Mar Ecol Prog Ser, 11, pp.299~308 (1983)
- Hoppe HG, "Use of fluorogenic model substrates for extracelllular enzyme activity (EEA) measurement of bacteria. In: Handbook of Methods in Aquatic Microbial Ecology", pp.423~432 (1993), Lewis Publishers
- Howarth RW, "The regulation of decomposition and heterotrophic microbial activity in salt marsh soils: a review. In: Estuarine Comparisons", pp.103~127 (1982), Academic Press
- Hungate BA, "Soil microbiota in two annual grasslands: responses to elevated atmospheric CO2", Oecologia, 124, pp.589~598 (2000)
- Hungate BA, "Assessing the effect of elevated carbon dioxide on soil carbon: a comparison on four meta-analyses", Global Change Biol, 15, pp.2020~2034 (2009)
- Summary for policymakers. In: Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change, "IPCC", Cambridge University Press, ~ (2007)
- Janus LR, "Elevated atmospheric CO2 alters soil microbial communities associated with trembling aspen (Populus tremuloides) roots", Microb Ecol, 50, pp.102~109 (2005)
- Keller JK, "Elevated CO2 affects porewater chemistry in a brackish marsh", Biogeochemistry, 96, pp.101~117 (2009)
- Kennedy AC, "Soil microbial diversity and the sustainability of agricultural soils", Plant Soil, 170, pp.75~86 (1995)
- Kleikemper J, "Sulfate-reducing bacterial community response to carbon source amendments in contaminated aquifer microcosms", FEMS Microbiol Ecol, 42, pp.109~118 (2002)
- Langley JA, "Elevated CO2 stimulates marsh elevation gain, counterbalancing sea-level rise", Proc Natl Acad Sci USA, 통권 : 106, pp.6182~6186 (2009)
- Leloup J, "Dynamics of sulfate-reducing microorganisms (dsrAB genes) in two contrasting mudflats of the Seine estuary (France)", Microb Ecol, 50, pp.307~314 (2005)
- Lenssen GM, "Interactive effects of atmospheric CO2 enrichment, salinity and flooding on growth of C3 (Elymus athericus) and C4 (Spartina anglica) salt marsh species", Vegetatio, 104, pp.379~388 (1993)
- Li JH, "Evapotranspiration and water use efficiency in a Chesapeake Bay wetland under carbon dioxide enrichment", Global Change Biol, 16, pp.234~245 (2010)
- Long SP, "Rising atmospheric carbon dioxide: plants face the future", Annu Rev Plant Biol, 55, pp.591~628 (2004)
- Marsh AS, "Effect of elevated CO2 on carbon pools and fluxes in a brackish marsh", Estuaries, 28, pp.694~704 (2005)
- McMahon PB, "Microbial production of organic acids in aquitard sediments and its role in aquifer geochemistry", Nature, 349, pp.233~235 (1991)
- Meyer-Reil LA, "Ecological aspects of enzymatic activity in marine sediments. In: Microbial Enzymes in Aquatic Environments", pp.84~95 (1991), Springer-Verlag
- Mooney HA, "Predicting ecosystem responses to elevated CO2 concentrations", BioScience, 41, pp.96~104 (1991)
- Moore B 3rd, "The oceans, carbon dioxide, and global climate change", Oceanus, 29, pp.9~15 (1986)
- Neubauer SC, "Transport of dissolved inorganic carbon from a tidal freshwater marsh to the York River estuary", Limnol Oceanogr, 48, pp.299~307 (2003)
- Paterson E, "Effect of elevated atmospheric CO2 concentration on C-partitioning and rhizosphere C-flow for three plant species", Soil Biol Biochem, 28, pp.195~201 (1996)
- Rillig MC, "Plant species-specific changes in root-inhabiting fungi in a California annual grassland: responses to elevated CO2 and nutrients", Oecologia, 113, pp.252~259 (1998)
- Sousa AI, "Contribution of Spartina maritima to the reduction of eutrophication in estuarine systems", Environ Pollut, 156, pp.628~635 (2008)
- Treseder KK, "Black boxes and missing sinks: fungi in global change research", Mycol Res, 104, pp.1281~1283 (2000)
- Wolf AA, "An oxygen-mediated positive feedback between elevated carbon dioxide and soil organic matter decomposition in a simulated anaerobic wetland", Global Change Biol, 13, pp.2036~2044 (2007)
- Zak DR, "Atmospheric CO2 and the composition and function of soil microbial communities", Ecol Appl, 10, pp.47~59 (2000)