Characterization of Cholesterol Lowering Lactic Acid Bacteria Isolated from Palm Wine and Maize Beer and Assessment of Their Use in the Production of Probiotic Papaya Juice
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Elevated serum cholesterol is a main risk factor for heart disorders. Most probiotic products administered to lower cholesterol are dairy products which are not suitable for lactose-intolerant individuals. In this study, we assessed the cholesterol-lowering efficacy of LAB isolated from traditionally fermented drinks in dietinduced rats and determine their efficacy in the production of non-dairy, probiotic formulations using papaya juice. LAB were isolated from palm wine and corn beer on MRS agar using a pour-plate technique. Identification was carried out using 16S rRNA gene sequencing. A hypercholesterolemia model in which diet-induced Wistar albino rats were assigned into four groups was established. Oral gavage was carried out for 30 days. On the 31st day, the rats were dissected and the serum lipid profile was analyzed using biochemical kits. A 106 cfu/ml of a 24-h-old culture of selected lactobacilli was used to inoculate papaya juice and incubated at 37℃. Microbial and chemical changes were assessed during papaya fermentation and after four weeks of cold storage. Two selected isolates (Pw1 and Cb4) had in vitro cholesterol reduction of > 80%. These two isolates lowered lipid profile (triglyceride, total cholesterol, LDL-c) significantly, and increased HDL-c levels (p < 0.5) in the rat sera. Phylogenetic analysis showed that Pw1 was 98.86% similar to Limosilactobacillus fermentum, while Cb4 was 99.54% similar to Enteroccocus faecium. Both strains fermented papaya juice with cell viability reaching 8.92 × 108 cfu/ml and 25.3 × 108 cfu/ml respectively, and were still viable after 4 weeks of cold storage.
Abstract
Elevated serum cholesterol is a main risk factor for heart disorders. Most probiotic products administered to lower cholesterol are dairy products which are not suitable for lactose-intolerant individuals. In this study, we assessed the cholesterol-lowering efficacy of LAB isolated from traditionally fermented drinks in dietinduced rats and determine their efficacy in the production of non-dairy, probiotic formulations using papaya juice. LAB were isolated from palm wine and corn beer on MRS agar using a pour-plate technique. Identification was carried out using 16S rRNA gene sequencing. A hypercholesterolemia model in which diet-induced Wistar albino rats were assigned into four groups was established. Oral gavage was carried out for 30 days. On the 31st day, the rats were dissected and the serum lipid profile was analyzed using biochemical kits. A 106 cfu/ml of a 24-h-old culture of selected lactobacilli was used to inoculate papaya juice and incubated at 37℃. Microbial and chemical changes were assessed during papaya fermentation and after four weeks of cold storage. Two selected isolates (Pw1 and Cb4) had in vitro cholesterol reduction of > 80%. These two isolates lowered lipid profile (triglyceride, total cholesterol, LDL-c) significantly, and increased HDL-c levels (p < 0.5) in the rat sera. Phylogenetic analysis showed that Pw1 was 98.86% similar to Limosilactobacillus fermentum, while Cb4 was 99.54% similar to Enteroccocus faecium. Both strains fermented papaya juice with cell viability reaching 8.92 × 108 cfu/ml and 25.3 × 108 cfu/ml respectively, and were still viable after 4 weeks of cold storage.
참고문헌 (56)
- Eidgahi EZ, "Assessment of risk factors for the cardiovascular diseases in people visiting a tertiary care hospital for routine medical check-up", J. Cardiovasc. Dis. Res, 9, pp.32~35 (2018)
- "Cardiovascular disease" (2017)[URL]
- Teo K, "Prevalence of a healthy lifestyle among individuals with cardiovascular disease in high-, middle-and low-income countries: The Prospective Urban Rural Epidemiology (PURE) study", JAMA, 309, pp.1613~1621 (2013)
- Karthiyaini Damodharan, "Functional Probiotic Characterization and In Vivo Cholesterol-Lowering Activity of Lactobacillus helveticus Isolated from Fermented Cow Milk", Journal of Microbiology and Biotechnology, 26(10), pp.1675~1686 (2016), 한국미생물·생명공학회[DOI]
- Costabile A, "An in vivo assessment of the cholesterol-lowering efficacy of Lactobacillus plantarum ECGC 13110402 in normal to mildly hypercholesterolaemic adults", PLoS One, 12, pp.e0187964~ (2017)
- Leuschner RG, "Qualified presumption of safety (QPS): a generic risk assessment approach for biological agents notified to the European Food Safety Authority (EFSA)", Trends Food Sci. Technol, 21, pp.425~435 (2010)
- Hill C, "The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic", Nat. Rev. Gastroenterol. Hepatol, 11, pp.506~514 (2014)
- Lv L-X, "Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 improves acute liver injury induced by D-galactosamine in rats", Appl. Microbiol. Biotechnol, 98, pp.5619~5632 (2014)
- Mingmongkolchai S, "Bacillus probiotics: an alternative to antibiotics for livestock production", J. Appl. Microbiol, 124, pp.1334~1346 (2018)
- Parveen S, "Fermented cereal from indigenous raw materials", Pakistan J. Nutr, 5, pp.289~291 (2003)
- Patel A, "Probiotic fruit and vegetable juices-recent advances and future perspective", Int. Food Res. J, 24, pp.1850~1857 (2017)
- Poli A, "Pharmacology of nutraceuticals with lipid lowering properties", High Blood Press. Cardiovasc. Prev, 26, pp.113~118 (2019)
- Szilagyi A, "Lactose intolerance, dairy avoidance, and treatment options", Nutrients, 10, pp.1994~ (2018)
- Zakaria L, "Diversity of colletotrichum species associated with anthracnose disease in tropical fruit crops-A review", Agriculture, 11, pp.297~ (2021)
- Njoya M, "Physicochemical and sensory properties of pawpaw (Carica papaya Linn)flavoured yoghurt", Afr. J. Food Agric. Nutr. Dev, 17, pp.12311~12324 (2017)
- Höfer T, "Stem-cell dynamics and lineage topology from in vivo fate mapping in the hematopoietic system", Curr. Opin. Biotechnol, 39, pp.150~156 (2016)
- Onuche P, "Palm wine tapping methods among idoma and tiv ethnic groups of Benue state, Nigeria:implications on conservation of palm trees (Elaeis guineensis)", J. Environ. Issues Agric. Dev. Ctries, 4, pp.86~ (2012)
- Guan X, "Screening and characterization of lactic acid bacterial strains that produce fermented milk and reduce cholesterol levels. brazilian", J. Microbiol, 48, pp.730~739 (2017)
- Pereira DI, "Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut", Appl. Environ. Microbiol, 68, pp.4689~4693 (2002)
- Ngongang EFT, "Isolation and identification of cholesterol lowering probiotic bacteria from palm wine (Raffia mambillensis)", J. Microbiol. Res, 6, pp.93~102 (2016)
- Al-Saleh A, "Bile salts and acid tolerance and cholesterol removal from media by some lactic acid bacteria and Bifidobacteria", J. Saudi Soc. Food Nutr, 1, pp.1~17 (2006)
- Mulaw G, "In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products", Int. J. Microbiol, 2019, pp.7179514~ (2019)
- Boubezari MT, "Bacteriocinogenic properties of Escherichia coli P2C isolated from pig gastrointestinal tract: purification and characterization of microcin V", Arch. Microbiol, 200, pp.771~782 (2018)
- Kimura M, "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16, pp.111~120 (1980)
- Kumar S, "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol. Biol. Evol, 33, pp.1870~1874 (2016)
- Friedewald WT, "Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge", Clin. Chem, 18, pp.499~502 (1972)
- Mousavi Z, "Fermentation of pomegranate juice by probiotic lactic acid bacteria", World J. Microbiol. Biotechnol, 27, pp.123~128 (2011)
- Bennani S, "Characterization and identification of lactic acid bacteria isolated from Moroccan raw cow’s milk", J. Mater. Environ. Sci, 8, pp.4934~4944 (2017)
- Tongwa QM, "Evaluation of probiotic potential of lactic acid bacteria isolated from Raphia palm wine (Raffia mambillensis)", South Asian J. Res. Microbiol, 3, pp.1~14 (2019)
- Fossi BT, "Lactic acid bacteria from traditionally processed corn beer and palm wine against selected food-borne pathogens isolated in south west region of Cameroon", Afr. J. Microbiol. Res, 10, pp.1140~1147 (2016)
- Ding W, "Screening for lactic acid bacteria in traditional fermented Tibetan yak milk and evaluating their probiotic and cholesterol-lowering potentials in rats fed a high-cholesterol diet", J. Functi. Foods, 32, pp.324~332 (2017)
- Belviso S, "In vitro cholesterollowering activity of Lactobacillus plantarum and Lactobacillus paracasei strains isolated from the Italian Castelmagno PDO cheese", Dairy Sci. Technol, 89, pp.169~176 (2009)
- Xu Y, "Exopolysaccharides produced by lactic acid bacteria and Bifidobacteria: Structures, physiochemical functions and applications in the food industry", Food Hydrocolloids, 94, pp.475~499 (2019)
- Gilliland S, "Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct", J. Dairy Sci, 67, pp.3045~3051 (1984)
- Ishimwe N, "The perspective on cholesterol‐lowering mechanisms of probiotics", Mol. Nutr. Food Res, 59, pp.94~105 (2015)
- Tilahun B, "Isolation and molecular identification of lactic acid bacteria using 16s rRNA genes from fermented Teff (Eragrostis tef (Zucc.))dough", Int. J. Food Sci, 2018, pp.8510620~ (2018)
- Ranadheera CS, "Probiotic delivery through fermentation: Dairy vs. nondairy beverages", Fermentation, 3, pp.67~ (2017)
- Fossi BT, "Probiotic properties of lactic acid bacteria isolated from fermented sap of palm tree (Elaeis guineensis)", J. Microbiol. Antimicrob, 7, pp.42~52 (2015)
- Sahadeva R, "Survival of commercial probiotic strains to pH and bile", Int. Food Res. J, 18, pp.1515~1522 (2011)
- Vasiee A, "Isolation, identification and characterization of probiotic Lactobacilli spp. from Tarkhineh", Int. Food Res. J, 21, pp.2487~ (2014)
- Sirilun S, "Characterisation of non human origin probiotic Lactobacillus plantarum with cholesterol-lowering property", Afr. J. Microbiol. Res, 4, pp.994~1000 (2010)
- Mandal S, "Enhancement of survival of alginate‐encapsulated Lactobacillus casei NCDC 298", J. Sci. Food Agric, 94, pp.1994~2001 (2014)
- Park JE, "Lactobacillus plantarum LG 42 isolated from gajami sik‐hae decreases body and fat pad weights in diet‐induced obese mice", J. Appl. Microbiol, 116, pp.145~156 (2014)
- Xenoulis PG, "Lipid metabolism and hyperlipidemia in dogs", Vet. J, 183, pp.12~21 (2010)
- Carmena R, "Atherosclerosis: Evolving vascular biology and clinical implications", Circulation, 109, pp.2~7 (2004)
- Mach F, "2019 ESC", EAS Guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk:the task force for the management of dyslipidaemias of the European Society of Cardiology (ESC) and European Atherosclerosis Society (EAS), 2020, pp.41, 111~188
- Liu Y, "Relationship between the non-HDLc-to-HDLc ratio and carotid plaques in a high stroke risk population: a cross-sectional study in China", Lipids Health Dis, 19, pp.1~12 (2020)
- Jeon YB, "Characterization of juice fermented with Lactobacillus plantarum EM and its cholesterol‐lowering effects on rats fed a high‐fat and high‐cholesterol diet", Food Sci. Nutr, 7, pp.3622~3634 (2019)
- Lg O, "Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings", Int. J. Mol. Sci, 11, pp.2499~2522 (2010)
- Aspri M, "Review on non-dairy probiotics and their use in non-dairy based products", Fermentation, 6, pp.30~ (2020)
- Septembre-Malaterre A, "Fruits and vegetables, as a source of nutritional compounds and phytochemicals:Changes in bioactive compounds during lactic fermentation", Food Res. Int, 104, pp.86~99 (2018)
- Chen R, "Comparative evaluation of the antioxidant capacities, organic acids, and volatiles of papaya juices fermented by Lactobacillus acidophilus and Lactobacillus plantarum", J. Food Qual, 2018, pp.9490435~ (2018)
- Di Cagno R, "Effect of autochthonous lactic acid bacteria starters on health-promoting and sensory properties of tomato juices", Int. J. Food Microbiol, 128, pp.473~483 (2009)
- Žuntar I, "Safety of probiotics: functional fruit beverages and nutraceuticals", Foods, 9, pp.947~ (2020)
- Lebaka Veeranjaneya Reddy, "Production of Probiotic Mango Juice by Fermentation of Lactic Acid Bacteria", 한국미생물·생명공학회지, 43(2), pp.120~125 (2015), 한국미생물·생명공학회[DOI]
- 윤경영, "Probiotication of Tomato Juice by Lactic Acid Bacteria", The Journal of Microbiology, 42(4), pp.315~318 (2004), 한국미생물학회