Deciphering Key Genes of Proliferative and Secretory Phase Using Integrated Transcriptomics and Network Analysis
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Endometrium receptivity is a complex mechanism of intricate pathways that lead to the shift from the proliferative to the secretory phase. Our goal was to identify high-ranking differentially expressed genes and study the pathways associated with the phenomenon. Raw data were retrieved from six GEO datasets and 705 DEGs were identified through robust ranking aggregation after the integration of five datasets. 20 key genes were identified that were further re-validated in an additional dataset. Supporting evidence through the experimental references confirms them as major biomarkers of the shift from the proliferative to the secretory phase.
Abstract
Endometrium receptivity is a complex mechanism of intricate pathways that lead to the shift from the proliferative to the secretory phase. Our goal was to identify high-ranking differentially expressed genes and study the pathways associated with the phenomenon. Raw data were retrieved from six GEO datasets and 705 DEGs were identified through robust ranking aggregation after the integration of five datasets. 20 key genes were identified that were further re-validated in an additional dataset. Supporting evidence through the experimental references confirms them as major biomarkers of the shift from the proliferative to the secretory phase.
참고문헌 (9)
- Petracco RG, "Global gene expression profiling of proliferative phase endometrium reveals distinct functional subdivisions", Reprod. Sci, 19, pp.1138~1145 (2012)
- Yanaihara A, "Differences in gene expression in the proliferative human endometrium", Fertil. Steril, 83, pp.1206~1215 (2005)
- Kelleher AM, "Integrative analysis of the forkhead box A2(FOXA2)cistrome for the human endometrium", FASEB J, 33, pp.8543~8554 (2019)
- "Geo accession viewer"[URL]
- Altmäe S, "Research resource: Interactome of Human embryo implantation:Identification of gene expression pathways, regulation, and integrated regulatory networks", Mol. Endocrinol, 26, pp.203~217 (2012)
- Chi R-pin A, "Human endometrial transcriptome and progesterone receptor cistrome reveal important pathways and epithelial regulators", J. Clin. Endocrinol. Metab, 105, pp.e1419~e1439 (2019)
- Giacomini E, "Global transcriptomic changes occur in uterine fluid-derived extracellular vesicles during the endometrial window for embryo implantation", Hum. Reprod, 36, pp.2249~2274 (2021)
- Sigurgeirsson B, "Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle", Biol. Reprod, 96, pp.24~33 (2017)
- Salamonsen Lois A, "Cyclical endometrial repair and regeneration", Development, 148 (2021)