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In-vitro Evaluation of Free Radical Scavenging Activities and Inflammatory Markers from LPS-Induced MH-S Cells by Xanthium strumarium L.

Evaluation of Free Radical Scavenging Activities and Inflammatory Markers from LPS-Induced MH-S Cells by Xanthium strumarium L.

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초록

Plants are a cornerstone of traditional medicine because they produce diverse chemical compounds with therapeutic potential. The genus Xanthium, particularly Xanthium strumarium, is renowned for its broad range of pharmacological effects but its anti-inflammatory properties in lipopolysaccharide (LPS)-induced MH-S cells remain underexplored. This research investigates anti-inflammatory and antioxidant activities of X. strumarium ethanol extract on LPS-stimulated MH-S cells. X. strumarium was extracted with ethanol and analyzed for its chemical composition using Gas chromatography–mass spectrometry (GC-MS). The antioxidant activity was evaluated through DPPH and ABTS assays while anti-inflammatory activity was evaluated in LPS stimulated MH-S cells by assessing nitric oxide (NO) production. Additionally, the expression of inflammatory cytokines and mediators (TNF-α, IL-1β, IL-6, iNOS, and COX-2) was assessed via RTPCR and qRT PCR. GC-MS analysis identified several major compounds in the extract, including fatty acids and phenolic compounds. Significant free radical scavenging activity was revealed in the antioxidant assays, particularly in the ABTS assay. In MH-S cells, X. strumarium extract dose-dependently reduced NO production and inhibited the expression of inflammatory cytokines and mediators without causing cytotoxicity. X. strumarium exhibits potent anti-inflammatory and antioxidant properties, as evidenced by its ability to reduce NO production and downregulate inflammatory cytokines and mediators in LPS-stimulated MH-S cells. These findings support the potential of X. strumarium as a natural anti-inflammatory agent and underscore its therapeutic potential in managing oxidative stress and inflammation. Future research should further elucidate the mechanistic pathways underlying these effects.

Abstract

Plants are a cornerstone of traditional medicine because they produce diverse chemical compounds with therapeutic potential. The genus Xanthium, particularly Xanthium strumarium, is renowned for its broad range of pharmacological effects but its anti-inflammatory properties in lipopolysaccharide (LPS)-induced MH-S cells remain underexplored. This research investigates anti-inflammatory and antioxidant activities of X. strumarium ethanol extract on LPS-stimulated MH-S cells. X. strumarium was extracted with ethanol and analyzed for its chemical composition using Gas chromatography–mass spectrometry (GC-MS). The antioxidant activity was evaluated through DPPH and ABTS assays while anti-inflammatory activity was evaluated in LPS stimulated MH-S cells by assessing nitric oxide (NO) production. Additionally, the expression of inflammatory cytokines and mediators (TNF-α, IL-1β, IL-6, iNOS, and COX-2) was assessed via RTPCR and qRT PCR. GC-MS analysis identified several major compounds in the extract, including fatty acids and phenolic compounds. Significant free radical scavenging activity was revealed in the antioxidant assays, particularly in the ABTS assay. In MH-S cells, X. strumarium extract dose-dependently reduced NO production and inhibited the expression of inflammatory cytokines and mediators without causing cytotoxicity. X. strumarium exhibits potent anti-inflammatory and antioxidant properties, as evidenced by its ability to reduce NO production and downregulate inflammatory cytokines and mediators in LPS-stimulated MH-S cells. These findings support the potential of X. strumarium as a natural anti-inflammatory agent and underscore its therapeutic potential in managing oxidative stress and inflammation. Future research should further elucidate the mechanistic pathways underlying these effects.

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