A Novel Approach for Assessing the Proteolytic Potential of Filamentous Fungi on the Example of Aspergillus spp.
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Proteolytic enzymes produced by filamentous fungi can degrade various fibrous and globular proteins along with other metabolites that may also find application in biotechnology. In this study, the effect of proteolytic enzymes of 22 Aspergillus strains on various proteins was investigated using protein-containing diagnostic media. Subsequently, a new parameter estimating secreted proteinases specificity towards fibrous or globular proteins without its advanced biochemical research - index of severity of proteolytic action (ISPA) - was suggested. This index determines mycozymes specificity in following manner: its value increases with greater affinity to fibrous proteins, decreases if there is higher affinity to globular proteins. ISPA value was the lowest (0.52) for Aspergillus domesticus, indicating the highest specificity to globular proteins, the highest one (1.26) for A. glaucus, whose proteinases best hydrolyzed fibrous proteins. However, the highest overall proteolytic potential was observed for Aspergillus melleus. The ability to produce acid, alkali and extracellular pigments was evaluated for all isolated strains as well.
Abstract
Proteolytic enzymes produced by filamentous fungi can degrade various fibrous and globular proteins along with other metabolites that may also find application in biotechnology. In this study, the effect of proteolytic enzymes of 22 Aspergillus strains on various proteins was investigated using protein-containing diagnostic media. Subsequently, a new parameter estimating secreted proteinases specificity towards fibrous or globular proteins without its advanced biochemical research - index of severity of proteolytic action (ISPA) - was suggested. This index determines mycozymes specificity in following manner: its value increases with greater affinity to fibrous proteins, decreases if there is higher affinity to globular proteins. ISPA value was the lowest (0.52) for Aspergillus domesticus, indicating the highest specificity to globular proteins, the highest one (1.26) for A. glaucus, whose proteinases best hydrolyzed fibrous proteins. However, the highest overall proteolytic potential was observed for Aspergillus melleus. The ability to produce acid, alkali and extracellular pigments was evaluated for all isolated strains as well.
참고문헌 (24)
- Popova EA, "Secretion of extracellular proteinases active against fibrillar proteins by micromycetes", Mosc. Univ. Biol. Sci. Bull, 72, pp.206~210 (2017)
- Osmolovskiy AA, "Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes", Biotechnol. Rep. (Amsterdam, Netherlands), 19, pp.e00265~ (2018)
- Timorshina S, "Keratinolytic properties of Aspergillus clavatus promising for biodegradation", Int. J. Environ. Res. Public Health, 19, pp.13939~ (2022)
- Osmolovskiy AA, "Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus", Microbiology, 86, pp.512~516 (2017)
- Arima K, "Milk clotting enzyme from microorganisms: Part I. Screening test and the identification of the potent fungus Part II. The enzyme production and the properties of crude enzyme", Agric. Biol. Chem, 31, pp.540~551 (1967)
- Sharkova TS, "Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes", Microbiology, 84, pp.359~364 (2015)
- Razzaq A, "Microbial proteases applications", Front. Bioeng. Biotechnol, 7, pp.110~ (2019)
- Tavano OL, "Biotechnological applications of proteases in food technology", Compr. Rev. Food Sci. Food Saf, 17, pp.412~436 (2018)
- Mótyán JA, "Research applications of proteolytic enzymes in molecular biology", Biomolecules, 3, pp.923~942 (2013)
- Fogarty WM, "Aspergillus", pp.177~218 (1994), Springer
- Wanderley MCDA, "Collagenolytic enzymes produced by fungi : A systematic review", Braz. J. Microbiol, 48, pp.13~24 (2017)
- Osmolovskiy AA, "Action of extracellular proteases of Aspergillus flavus and Aspergillus ochraceus micromycetes on plasma hemostasis proteins", Life, 11, pp.782~ (2021)
- Takenaka S, "Isolation and characterization of an aspartic protease able to hydrolyze and decolorize heme proteins from Aspergillus glaucus", J. Sci. Food Agric, 99, pp.2042~2047 (2019)
- Lambrecht BN, "Asthma and coagulation", New Eng. J. Med, 369, pp.1964~1966 (2013)
- Din G, "Characterization of organic acid producing Aspergillus tubingensis FMS1 and its role in metals leaching from soil", Geomicrobiol. J, 37, pp.336~344 (2020)
- Fang Z, "Keratinolytic protease : A green biocatalyst for leather industry", Appl. Microbiol. Biotechnol, 101, pp.7771~7779 (2017)
- Verma A, "Microbial keratinases : Industrial enzymes with waste management potential", Crit. Rev. Biotechnol, 37, pp.476~491 (2017)
- Chanalia P, "Applications of microbial proteases in pharmaceutical industry", Rev. Med. Microbiol, 22, pp.96~101 (2011)
- Landers CT, "Selective cleavage of fibrinogen by diverse proteinases initiates innate allergic and antifungal immunity through CD11b", J. Biol. Chem, 294, pp.8834~8847 (2019)
- Iadarola P, "Lung injury and degradation of extracellular matrix components by Aspergillus fumigatus serine proteinase", Exp. Lung Res, 24, pp.233~251 (1998)
- Hirokawa K, "An enzymatic method for the determination of hemoglobinA(1C)", Biotechnol. Lett, 27, pp.963~968 (2005)
- Oda Y, "Degradation of polylactide by commercial proteases", J. Polym. Environ, 8, pp.29~32 (2000)
- Lagashetti AC, "Fungal pigments and their prospects in different industries", Microorganisms, 7, pp.604~ (2019)
- Lin L, "Production of fungal pigments : Molecular processes and their applications", J. Fungi, 9, pp.44~ (2022)