Detection of microbial organisms on Apis mellifera L. beehives in palm garden, Eastern Thailand
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Background: Honey bees play a crucial role in pollination and ecological balance. Apis mellifera L. colonies, especially those located in specific geographic regions, such as the palm garden in Eastern Thailand, are susceptible to potential threats from microbial contaminants. Understanding and detecting microbial organisms in these beehives is essential for the preservation of bee health, honey production, and the broader ecosystem. However, the problem of microbial infection and antibiotic-resistant bacteria is more severe and continuously increasing, resulting in a health, economic, and social crisis. The purpose of this study is to determine the prevalence of microorganisms in A. mellifera beehives in palm gardens in Rayong province, Eastern Thailand. Results: Ten swabs in transport media were swabbed and obtained from different parts of each beehive (1 swab per beehive), for a total of 10 hives. Traditional microbial culture- based methods, biochemical tests, and antimicrobial susceptibility (disc-diffusion) tests were used to detect microbial organisms and antibiotic resistance in bacteria. The swab tests from nine beehives resulted in the detection of Gram-positive bacteria (63.64%), Gram-negative bacteria (27.27%), and fungi/yeast (9.09%). These microorganisms are classified as a group of coagulase-negative Staphylococcus spp. and made up 40.91% of the bacteria discovered. Other bacteria found were Coryneform bacteria (13.64%), Pantoea spp. (13.64%), Bacillus spp. (9.09%), yeast (9.09%), glucose non-fermentative Gram-negative bacilli (9.09%), and Pseudomonas spp. (4.55%). However, due to the traditional culture- based and 0biochemical tests usually used to identify the microbial organisms in clinical specimens and the limitation of identifying some environmental microbial species, the results of the antimicrobial susceptibility test cannot reveal if the organism is resistant or susceptible to the drug. Nevertheless, drug-sensitive inhibition zones were formed with each antibiotic agent. Conclusions: Overall, the study supports prevention, healthcare, and public health systems. The contamination of microorganisms in the beehives may affect the quality of honey and other bee products or even the health of the beekeeper. To avoid this kind of contamination, it is therefore necessary to wear personal protective equipment while harvesting honey and other bee products.
Abstract
Background: Honey bees play a crucial role in pollination and ecological balance. Apis mellifera L. colonies, especially those located in specific geographic regions, such as the palm garden in Eastern Thailand, are susceptible to potential threats from microbial contaminants. Understanding and detecting microbial organisms in these beehives is essential for the preservation of bee health, honey production, and the broader ecosystem. However, the problem of microbial infection and antibiotic-resistant bacteria is more severe and continuously increasing, resulting in a health, economic, and social crisis. The purpose of this study is to determine the prevalence of microorganisms in A. mellifera beehives in palm gardens in Rayong province, Eastern Thailand. Results: Ten swabs in transport media were swabbed and obtained from different parts of each beehive (1 swab per beehive), for a total of 10 hives. Traditional microbial culture- based methods, biochemical tests, and antimicrobial susceptibility (disc-diffusion) tests were used to detect microbial organisms and antibiotic resistance in bacteria. The swab tests from nine beehives resulted in the detection of Gram-positive bacteria (63.64%), Gram-negative bacteria (27.27%), and fungi/yeast (9.09%). These microorganisms are classified as a group of coagulase-negative Staphylococcus spp. and made up 40.91% of the bacteria discovered. Other bacteria found were Coryneform bacteria (13.64%), Pantoea spp. (13.64%), Bacillus spp. (9.09%), yeast (9.09%), glucose non-fermentative Gram-negative bacilli (9.09%), and Pseudomonas spp. (4.55%). However, due to the traditional culture- based and 0biochemical tests usually used to identify the microbial organisms in clinical specimens and the limitation of identifying some environmental microbial species, the results of the antimicrobial susceptibility test cannot reveal if the organism is resistant or susceptible to the drug. Nevertheless, drug-sensitive inhibition zones were formed with each antibiotic agent. Conclusions: Overall, the study supports prevention, healthcare, and public health systems. The contamination of microorganisms in the beehives may affect the quality of honey and other bee products or even the health of the beekeeper. To avoid this kind of contamination, it is therefore necessary to wear personal protective equipment while harvesting honey and other bee products.
참고문헌 (19)
- Anderson KE, "Hive-stored pollen of honey bees : many lines of evidence are consistent with pollen preservation, not nutrient conversion", Mol Ecol, 23(23), pp.5904~5917 (2014)[DOI]
- Becker K, "Coagulase-negative staphylococci", Clin Microbiol Rev, 27(4), pp.870~926 (2014)[DOI]
- Bell RL, "Recent and emerging innovations in Salmonella detection : a food and environmental perspective", Microb Biotechnol, 9(3), pp.279~292 (2016)[DOI]
- M45 Methods for antimicrobial dilution and disk susceptibility testing of infrequently Isolated or fastidious bacteria, "Clinical and Labortory Standaras Institute", Clinical and Labortory Standaras Institute, ~ (2015)
- M100 performance standards for antimicrobial susceptibility testing, "Clinical and Laboratory Standards Institute", Clinical and Laboratory Standards Institute, ~ (2022)
- Dadgostar P, "Antimicrobial resistance : implications and costs", Infect Drug Resist, 12, pp.3903~3910 (2019)[DOI]
- Foote HL, "Possible use of microorganisms in synthetic bee bread production", Am Bee J, 97, pp.476~478 (1957)
- Haydak MH, "Pollen-pollen substitutes-beebread", Am Bee J, 98(4), pp.145~146 (1958)
- Hölzel CS, "Unraveling the role of vegetables in spreading antimicrobial-resistant bacteria : a need for quantitative risk assessment", Foodborne Pathog Dis, 15(11), pp.671~688 (2018)[DOI]
- Khalifa SAM, "Overview of bee pollination and its economic value for crop production", Insects, 12(8), pp.688~ (2021)[DOI]
- Lannutti L, "Molecular detection and differentiation of arthropod, fungal, protozoan, bacterial and viral pathogens of honeybees", Vet Sci, 9(5), pp.221~ (2022)[DOI]
- Layla S, "Prevelance and antibiotic susceptibility patterns of Pantoea spp. isolated form clinical and environmental sources in Iraq", Int J ChemTech Res, 9(8), pp.430~437 (2016)
- "Kirby-Bauer Disk Susceptibility Test" (2023)[URL]
- "The Surprisingly Sophisticated Ways That Honey Bees Fight Disease" (2020)[URL]
- Nguyen AT, "Screening food for Bacillus cereus toxins using whole genome sequencing", Food Microbiol, 78, pp.164~170 (2019)[DOI]
- Sandot A, "Risk of bronchial complications after lung transplantation with respiratory corynebacteria. Results From a Monocenter Retrospective Cohort Study", Transpl Int, 36, pp.10942~ (2023)[DOI]
- Saravanan A, "Methods of detection of food-borne pathogens : a review", Environ Chem Lett, 19, pp.189~207 (2021)[DOI]
- Serwecińska L, "Antimicrobials and antibiotic-resistant bacteria : a risk to the environment and to public health", Water, 12(12), pp.3313~ (2020)[DOI]
- Yadav SK, "Emergence of multidrug-resistant non-fermentative gram negative bacterial infection in hospitalized patients in a tertiary care center of Nepal", BMC Res Notes, 13(1), pp.319~ (2020)[DOI]