어류병원성 그람음성세균 Edwardsiella tarda와 Vibrio anguillarum에 대한 칠피의 항균활성물질
The antimicrobial compound of Rhus verniciflua barks against fish pathogenic gram-negative bacteria, Edwardsiella tarda and Vibrio anguillarum
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초록
본 연구에서는 주요 어류 병원성 그람음성세균인 E. tarda 및 V. anguillarum에 대하여 14 종의 생약 추출물의 항균활성을 검색하였다. 그 결과, 감초, 지유 및 칠피 추출물이 항균활성을 나타냄을 밝혔다. 그 중, 가장 우수한 항균활성을 나타낸 칠피 추출물에 대하여 활성지향적인 분리기법을 적용하여 활성분획물인 EtOAc분획물로부터 항균활성물질 1종을 분리하고 페놀성 화합물인 methyl gallate로 동정하였다. methyl gallate는 실험에 사용된 E. tarda 및 V. anguillarum의 균주들의 증식을 효과적으로 저해하였으며 이에 대한 MIC는 모두 1 mg/ml로 측정되었다.
Abstract
To obtain antimicrobial compounds against fish pathogenic bacteria from natural products, 80 % methanolic extracts from 14 species of medicinal plant were screened for antimicrobial activity against fish pathogenic bacteria, Edwardsiella tarda and Vibrio anguillarum. Among them, Glycyrrhiza glabra, Rhus verniciflua and Sanguisorba officinalis were effective for growth inhibition of Gram-negative bacteria, both E. tarda YSF and V. anguillarum YSR. Through the activity-guided isolation for R. verniciflua extract that exhibited the highest antimicrobial activity among three extracts, one antimicrobial compound (1) was isolated and identified as methyl-3,4,5-trihydroxybenzoate, or methyl gallate. This compound significantly inhibited the growth of tested strains of both E. tarda and V. anguillarum exhibiting MIC of 1 mg/ml for each strain.
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