양식 조피볼락(Sebastes schlegeli)의 Epitheliocystis, Microcotyle sebastes 및 미동정 생물체의 혼합감염
A mixed infection of epitheliocystis, Microcotyle sebstes and unknown aetiology in cultured rockfish (Sebastes schlegeli) from farms in Huksan Island
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흑산도 근해의 가두리 양식장에서 아가미에 백색 결절을 주 증상으로 하는 조피볼락이 발견 되었다. 간과 신장에서는 병원균의 검출과 병리 조직학적인 변화가 전혀 발견되지 않았다. 아가 미의 경우, epitheliocystis, Microcotyle sebastes 및 아가미 새변의 연골내에 미동정 생물(unknown aetiology)의 cyst가 확인되었다. Epitheliocystis의cyst크기는 약 44.1-68.9 ㎛이었고 주로 새변의 끝과 중간부위에 기생하고 있었다. 그러나 새변 내의 cyst형성에도 불구하고 숙주의 반응은 미 약했다. M. sebastes는 2차새변에 고착기를 이용 하여 달라붙어있었으며, 이로 인하여 상피세포 의 증생이 발생하였다. 미동정 생물체의 경우, cyst는 호산성의 물질로 충만되어 있었고 cyst전 체가 연골에 의해 싸여 있는 형태를 보였다. 하 지만 이러한 cyst의 형성에도 불구하고 새변의 크기가 증대된 것 이외에는 별다른 병리학적인 증상은 보이지 않았다. 이들 기생충들의 의한 숙 주세포의 조직반응은 미약하여 폐사는 유발되지 않았지만, 호흡과 삼투압 조절에는 장애가 있을 것으로 추정된다.
Abstract
Fifteen diseased rockfish (Sebastes schlegeli) were collected from 4 net-cage farms in Huksan island. Some round transparent nodules were observed on gill filaments in fish. Bacteriological and histopathological examinations on the diseased fish were performed. Any pathogenic bacteria could not be solated from the internal tissues and histopathological alterations were not found in any of the tissues except the gills. Rock fish were infected with epitheliocystis, Microcotyle sebastes and unknown aetiology on the gills. Cysts of epitheliocystis measuring 44.1-68.9 ㎛ in diameter were mainly found in the distal portion of the gill filaments and sometimes in the middle portion. But host responses were moderate in spite of formation of the epithelial cysts. Some Microcotyle sebastes captured the secondary filaments with clamps on the opisthohaptor. Attachment of the parasites provoked epithelial proliferation and resulted in gill lamellar fusion. Cysts of unknown origins measuring 26-425 ㎛ in diameter were found in the cartilage of gill filaments. These cysts contained eosinophilic materials and proliferation of cartilage cells was confirmed around the cysts. But there was no significant pathological changes associated with the presence of the cysts except enlargement of the gill filament with the cysts.
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