넙치 혈청의 보존 조건이 ELISA 결과에 미치는 영향
Effect of storage conditions of olive flounder Paralichthys olivaceus serum on enzyme-linked immunosorbent assay
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초록
본 연구에서는 넙치 혈청의 보존 조건이 ELISA 결과에 미치는 영향을 확인하기 위하여, 항 BSA 넙치 혈청을 이용해 보존 온도, 보존 기간 및 해동 횟수에 따른 ELISA값을 비교하였다. 넙치 혈청을 보존 온도 (-80, -20, 4 및 20℃) 및 기간 (1, 30, 69 및 124일)에 따른 ELISA OD값을 비교한 결과, 모든 시료에서 뚜렷한 OD값 의 차이는 확인되지 않았다. -80℃ 및 -20℃에 보존된 혈청을 사용하여 해동횟수 (1, 5, 10회)에 따른 ELISA OD값을 비교한 결과, 해동을 반복한 혈청 및 미동결 혈청의 OD값은 뚜렷한 차이를 보이지 않았다. 이상의 결과로 넙치 혈청은 -80, -20, 4 및 20℃에서 124일간 보존하거나 혈청을 10회 반복 해동하여도 ELISA 결과에 영향을 끼치지 않는 것이 확인되었다.
Abstract
Antibody-detection enzyme-linked immunosorbent assay (ELISA) was performed to determine whether the absorbance value of ELISA is influenced by the different serum storage conditions of olive flounder Paralichthys olivaceus. Flounder antiserum to bovine serum albumin was stored at -80, -20, 4 and 20℃ for 1, 30, 69 and 124 days, respectively. In addition, the flounder antisera were frozen at -80 and -20℃, respectively and then repeated 1, 5 and 10 thaw-freeze cycles. No significant difference was shown in ELISA optical density (O.D.) values of sera, which were stored at the above mentioned storage conditions during 124 days. ELISA O.D. values of unfrozen serum samples, which were previously stored at 4℃, were almost similar to those of sera undergoing 1, 5, and 10 freeze-thaw cycles after stored at -80 or -20℃. In conclusion, the ELISA O.D. values of flounder sera were not affected by various storage conditions: different temperatures (-80, -20, 4 and 20℃), durations of storage (1, 30, 69 and 124 days), and repeated thaw-freeze cycles (1, 5, and 10 times).
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