Evaluation of enzyme-linked immunosorbent assay (ELISA) for detection of olive flounder antibodies to viral hemorrhagic septicemia virus (VHSV, genotype IVa) using two Novirhabdovirus antigens
1
조회
0
다운로드
0
인용
0
참고문헌
초록
An enzyme-linked immunosorbent assay (ELISA) with two Novirhabdovirus antigens (viral hemor-rhagic septicemia virus, VHSV and infectious hematopoietic necrosis virus, IHNV) was used to detect specific antibodies against VHSV from olive flounder (Paralichthys olivaceus) sera. In ELISA plates with VHSV culture supernatants (VHSV-Ag plate), optical density (OD) values for sera from olive flounder with VHS history (VHS sera) ranged from 0.64±0.36, and those of sera from fish without VHS history (non-VHS sera) ranged from 0.26±0.26. In IHNV-Ag plate, the OD values (0.43±0.28) for VHS sera were quite low compared to those in VHSV-Ag plates, while the OD values for non-VHS sera were almost similar. When the OD values for each serum were calculated by subtracting the OD values in the IHNV-Ag plate from those in the VHSV-Ag plate, the corrected OD values were significantly different between VHS sera and non-VHS sera. The results were completely in line with fish histories of VHS epizootics. It was considered that the corrected OD values may represent the true values recognized by VHSV-specific antibodies.
Abstract
An enzyme-linked immunosorbent assay (ELISA) with two Novirhabdovirus antigens (viral hemor-rhagic septicemia virus, VHSV and infectious hematopoietic necrosis virus, IHNV) was used to detect specific antibodies against VHSV from olive flounder (Paralichthys olivaceus) sera. In ELISA plates with VHSV culture supernatants (VHSV-Ag plate), optical density (OD) values for sera from olive flounder with VHS history (VHS sera) ranged from 0.64±0.36, and those of sera from fish without VHS history (non-VHS sera) ranged from 0.26±0.26. In IHNV-Ag plate, the OD values (0.43±0.28) for VHS sera were quite low compared to those in VHSV-Ag plates, while the OD values for non-VHS sera were almost similar. When the OD values for each serum were calculated by subtracting the OD values in the IHNV-Ag plate from those in the VHSV-Ag plate, the corrected OD values were significantly different between VHS sera and non-VHS sera. The results were completely in line with fish histories of VHS epizootics. It was considered that the corrected OD values may represent the true values recognized by VHSV-specific antibodies.
참고문헌 (20)
- Isshiki, T., "An outbreak of VHSV(viral hemorrhagic septicemia virus)infection in farmed Japanese flounder Paralichthys olivaceus in Japan", Dis Aquat Organ, 47(2), pp.87~99 (2001)[DOI]
- Kibenge, M. T., "Serological evidence of infectious salmon anaemia virus(ISAV)infection in farmed fishes, using an indirect enzyme-linked immunosorbent assay(ELISA)", Dis Aquat Organ, 51, pp.1~11 (2002)[DOI]
- Kim, W. S., "Non-specific adsorption of fish immunoglobulin M(IgM)to blocking reagents on ELISA plate wells", Dis Aquat Organ, 78, pp.55~59 (2007)[DOI]
- Kim, W. S., "Genotyping of Korean isolates of infectious hematopoietic necrosis virus(IHNV)based on the glycoprotein gene", Arch Virol, 152, pp.2119~2124 (2007)[DOI]
- Kim, W. S., "Detection of specific antibodies against infectious hematopoietic necrosis virus from rainbow trout sera by ELISA using two novirhabdoviruses", Fish Pathol, 43(3), pp.112~116 (2008)[DOI]
- Kim, W. S., "An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea", Aquaculture, 296(1-2), pp.165~168 (2009)[DOI]
- Kim, W. S., "Genetic positioning of Korean viral hem-orrhagic septicemia virus(VHSV)from cultured and wild marine fishes", J Fish Pathol, 24(1), pp.1~9 (2011)[DOI]
- Kim, W. S., "Korean and Japanese isolates of viral hemorrhagic septicemia virus from olive flounder are pathogenic to rainbow trout fry", Fish Pathol, 46, pp.112~115 (2011)[DOI]
- Kimura, T., "A new rhabdovirus isolated in Japan from cultured hirame(Japanese flounder)Paralichthys olivaceus and ayu Plecoglossus altivelis", Dis Aquat Organ, 1, pp.209~217 (1986)[DOI]
- Knope, K., "Evaluation of an ELISA and immuno-blotting for studying the humoral immune response in Anguillicola crassus infected European eel An-guilla anguilla", Dis Aquat Organ, 43, pp.39~48 (2000)[DOI]
- LaPatra, S. E., "The use of serological techniques for virus surveillance and certification of finfish", Annu Rev Fish Dis, 6, pp.15~28 (1996)[DOI]
- Millard, E. V., "Detection of viral hemorrhagic septicemia virus-IVb antibodies in sera of musk-ellunge Esox masquinongy using competitive ELISA", Dis Aquat Organ, 108, pp.187~199 (2014)[DOI]
- Nishizawa, T., "Genetic relatedness among Japa-nese, American and European isolates of viral hem-orrhagic septicemia virus(VHSV)based on partial G and P genes", Dis Aquat Organ, 48(2), pp.143~148 (2002)[DOI]
- Olesen, N. J., "Detection of rainbow trout antibody to Egtved virus by en-zyme-linked immunosorbent assay(ELISA), im-munofluorescence(IF), and plaque neutralization tests(50% PNT)", Dis Aquat Organ, 10, pp.31~38 (1991)[DOI]
- Skall, H. F., "Viral haemorrhagic septicaemia virus in marine fish and its im-plications for fish farming-a review", J Fish Dis, 28(9), pp.509~529 (2005)[DOI]
- Smail, D., "Fish diseases and disorders", pp.123~146 (1999), CABI Publishing
- Trdo, N., "Virus Taxonomy", pp.623~653 (2005), Elsevier
- Watanabe, K., "Control strategy for viral nervous necrosis of barfin flounder", Fish Pathol, 33, pp.445~446 (1998)[DOI]
- WOAH, "Manual of diagnostic tests for aquatic animals" (2021), World Organisation for Animal Health
- Wolf, K., "Fish viruses and fish viral diseases", pp.217~249 (1988), Cornell University Press