치어기 넙치 사료내 Seleno-yeast 수준에 따른 조직학적 변화
Effects of dietary seleno-yeast levels on histological responses in juvenile olive flounder Paralichthys olivaceus
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본 실험은 치어기 넙치 사료 내 셀레노이스트 첨가에 따른 영향을 평가하기 위하여 조직학적 반응을 살펴보았다. 사료 내 셀레늄은 각각 0ppm, 1ppm, 3ppm 및 5ppm의 총 4개 실험구로 설정하였으나 실험사료 분석을 통하여 사료내 셀레늄의 함량은 각각 0.56, 1.07, 2.86 및 4.56 mg Se/kg으로 나타났다. (Se0.56, Se1.07, Se2.86, 및 Se4.56). Se1.07 실험구의 신장에서 사구체의 혈구세포가 점진적인 팽창을 보여주고, 일부 세뇨관의 상피세포들의 파열도 나타나기 시작하였으며 대식세포도 점차적으로 커지는 것을 알 수 있었다. Se0.56 실험구의 간에서 정상적인 형태를 갖추고 있다. 농도가 증가될수록 모세 혈관 내 혈구의 팽창, 췌장 효소원 과립의 감소, 간세포의 팽창이 점진적으로 진행되는 볼 수 있었다. 그리고 Se1.07 실험구에서 일부 비정상적인 새판을 가지는 것으로 나타났으나 Se0.56 실험구와 크게 차이가 없는 것으로 관찰되었다. 이상의 세 기관의 조직학적 연구에서 치어기 넙치 사료내 첨가제로서 셀레노이스트의 적정 첨가량은 1.07 mg/kg 이하 수준이 적당한 것으로 사료된다.
Abstract
This study was conducted to investigate the effects of dietary seleno-yeast on histological response in juvenile olive flounder Paralichthys olivaceus. Fish averaging 4.0 ± 0.1 g (mean ± SD) were fed one of the four semi-purified diets containing 0.56, 1.07, 2.86 and 4.56 mg Selenoyeast/kg diet (Se0.56, Se1.07, Se2.86 and Se4.56, respectively) in triplicates for 12 weeks. Swelling of blood cell in glomerulus, the rupture of some epithelial cell in the renal tubules and enlarged macrophage were observed in the Se1.07 group. The hepatopancreas had hepatic cell, capillary and zymogen in the pancreas and was normal in the Se0.56 group. Swelling of hepatocyte gradually decreased with increase in selenoyeast supplementation. The gill lamellae showed partial abnormal condition (terminal clubbing) in the Se0.56 group, but there were little differences between these and other treatments. These results indicated that the optimum dietary supplementation level of selenoyeast in juvenile olive flounder, Paralichthys olivaceus, could be less than 1.07 mg selenoyeast/kg diet based on histological responses of the fish.
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