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국내 양식 무지개송어에서 분리한 IHNV glycoprotein의 유전자 분석

Phylogenetic analysis of infectious hematopoietic necrosis virus (IHNV) isolated from cultured rainbow trout Oncorhynchus mykiss in Korea

김형준
수산생명의학회 (Aquatic Life Medical Science)|2010.04|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제23권 제1호|pp. 1-8

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참고문헌

키워드

KoreaGlycoproteinPhylogenetic analysisRainbow troutIHNV

초록

무지개 송어 발안란의 이동 이력과 IHNV G gene 염기서열의 계통학적 분석에 의해 IHNV의 국내 이동에 대해서 처음으로 조사되었다. IHNV RtWanju09 분리주는 IHNV RtPy91과 RtJe00의 JRt Shizuoka lineage에 속하는 것으로 나타났으며, 강원도에서 분리된 IHNV RtPy91과 RtWanju09 사이의 G gene의 유전적 차이는1.77%였고, 약 20년간 우리나라의 JRt Shizuoka lineage에 속하는 한국 분리주들은 최대 3.03% 의 유전적 차이를 나타내 계속적으로 바이러스가 진화하는 것을 알 수 있었다. 또한 전라북도 지역에서 분리된 IHNV RtWanju09 분리주는 IHNV가 오염된 강원도 지역산 발안란이 전라북도에 전파되었을 것으로 사료된다.

Abstract

Infectious hematopoietic necrosis virus (IHNV) is the causative agent of IHN, one of the most serious viral diseases of salmonid fish. In this study, glycoprotein (G) gene nucleotide sequence of isolated IHNV RtWanju09 from Jeollabuk-do province was analyzed to evaluate their genetic relatedness to worldwide isolates. As the result, it was revealed that IHNV RtWanju09 isolate belongs to JRt Shizuoka lineage with IHNV RtPy91 and RtJe00. The genetic diversity of G gene between RtWanju09 isolate and RtPy91 isolate from Gangwon-do province was 1.77% and maximum nucleotide diversity among the JRt Shizuoka lineage in Korea was 3.03% during the past 20 years, supporting that the continuous evolution has been occurred among JRt Shizuoka isolates. It was believed that IHNV RtWanju09 isolate has been introduced by the movement of contaminated eggs with IHNV from Gangwon-do to Jeollabuk-do by the reason that the eyed eggs in Jeollabuk-do province used to be obtained from Gangwon-do province. In this study, the domestic transfer of IHNV was firstly investigated by the transfer history of eggs and the phylogenetic analysis using IHNV glycoprotein gene sequence.

참고문헌 (25)

  1. Bootland, L.M., "Infectious hematopoietic necrosis virus. in: Fish diseases and disorders; Viral, bacterial and fungal infections, Vol. 3", pp.57~121 (1999), CABI Publishing
  2. Enzmann, P.J., "Infectious hematopoietic necrosis virus: monophyletic origin of European isolates from North American genogroup M", Dis. Aquat. Org., 66, pp.187~195 (2005)
  3. Garver, K.A., "Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin", Dis. Aquat. Org., 55, pp.187~203 (2003)
  4. Gilmore R.D.Jr., "The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus", Virology, 167, pp.644~648 (1988)
  5. Kim, C.H., "Neutralization-resistant variants of infectious hematopoietic necrosis virus have altered virulence and tissue tropism", J. Virol., 68, pp.8447~8453 (1994)
  6. 김기홍, "무지개송어 성어에 대량 폐사를 유발하는 IHNV의 분리와 특성", 한국어병학회지, 16(2), pp.81~90 (2003), 한국어병학회
  7. Kim, W.S., "Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene", Arch. Virol., 152, pp.2119~2124 (2007)
  8. Kimura, T., "Viral diseases of fish in Japan", Annu. Rev. Fish Dis., 1, pp.67~82 (1991)
  9. Koener, J.F., "Nucleotide sequence of a cDNA clone carrying the glycoprotein gene of infectious hematopoietic necrosis virus, a fish rhabdovirus", J. Virol., 61, pp.1342~1349 (1987)
  10. Kurath, G, "Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping", J. Virol., 53, pp.469~476 (1985)
  11. Kurath, G., "Phylogeography of infectious hematopoietic necrosis virus in North America", J. Gen. Virol., 84, pp.803~814 (2003)
  12. Miller, T.A., "Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and culture cells", Dis. Aquat. Org., 34, pp.13~20 (1998)
  13. Mochizuki, M., "Virulence change of infectious hematopoietic necrosis against rainbow trout Oncorhynchus mykiss with viral molecular evolution", Fish Pathol., 44(4), pp.159~165 (2009)
  14. Morzunov, S.P., "The complete genome structure and phylogenetic relationship of infectious hematopoietic necrosis virus", Virus Res., 38, pp.175~192 (1995)
  15. Nichol, S.T., "Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus", Virus Res., 38, pp.159~173 (1995)
  16. Nishizawa, T., "Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene", Dis. Aquat. Org., 71, pp.267~272 (2006)
  17. Perrière, G., "WWW-Query: an online retrieval system for biological sequence banks", Biochimie., 78, pp.364~369 (1996)
  18. Schutze, H., "Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus", J. Gen. Virol., 76, pp.2519~2527 (1995)
  19. Tamura, K., "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Mol. Biol. Evol., 24, pp.1596~1599 (2007)
  20. Thompson, J.D., "Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res., 22, pp.4673~4680 (1994)
  21. Thompson, J.D., "The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools", Nucleic Acids Res., 25, pp.4876~4882 (1997)
  22. Tordo, N., "Family Rhabdoviridae. in: Virus taxonomy, VIIIth report of the ICTV", pp.623~644 (2005), Elsevier, Academic Press
  23. Winton, J.R., "Recent advances in detection and control of infectious hematopoietic necrosis virus in aquaculture", Annu. Rev. Fish Dis., 1, pp.83~93 (1991)
  24. Wolf, K., "Infectious hematopoietic necrosis. in: Fish viruses and fish viral diseases", pp.83~114 (1988), Cornell University Press
  25. Yoshimizu, M., "Disease problems of salmonid fish in Japan caused by international trade", Rev. Sci. Tech., 15, pp.533~549 (1996)

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