Generation of heterologous proteins-expressing recombinant snakehead rhabdoviruses (rSHRVs) using reverse genetics
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Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.
Abstract
Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.
참고문헌 (14)
- Alonso, M., "The NV gene of snakehead rhabdovirus(SHRV)is not required for pathogenesis, and a heterologous glycoprotein can be incorporated into the SHRV envelope", J. Virol, 78, pp.5875~5882 (2004)
- Amarasinghe, G. K., "Taxonomy of the order Mononegavirales : update 2019", Arch. Virol, 164, pp.1967~1980 (2019)
- Gabor, K. A., "Mononegaviruses of Veterinary Importance. Volume I: Pathobiology and Molecular Diagnosis", pp.337~353 (2013), CAB International
- Herbert, B., "Impacts of epizootic ulcerative syndrome on subsistence fisheries and wildlife", Rev. Sci. Tech, 38, pp.459~475 (2019)
- Iberahim, N. A., "Aphanomyces invadans, the causal agent of epizootic ulcerative syndrome, is a global threat to wild and farmed fish", Fungal. Biol. Rev, 32, pp.118~130 (2018)
- Johnson, M. C., "Production of recombinant snakehead rhabdovirus : the NV protein is not required for viral replication", J. Virol, 74, pp.2343~2350 (2000)
- Kasornchandra, J., "Characteristics of three rhabdoviruses from snakehead fish Ophicephalus striatus", Dis. Aquat. Org, 13, pp.89~94 (1992)
- Kim, M. S., "Genetically engineered viral hemorrhagic septicemia virus(VHSV)vaccines", Fish Shellfish Immunol, 95, pp.11~15 (2019)
- Kim, M. S., "Generation of recombinant viral hemorrhagic septicemia virus(rVHSV)expressing two foreign proteins and effect of lengthened viral genome on viral growth and in vivo virulence", Mol. Biotechnol, 58, pp.280~286 (2016)
- Kurath, G., "Molecular cloning of the six mRNA species of in-fectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping", J. Virol., 53, pp.469~476 (1985)
- Phelan, P. E., "Characterization of snakehead rhabdovirus infection in zebrafish(Danio rerio)", J. Virol, 79, pp.1842~1852 (2005)
- Romero, A., "Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout(Oncorhynchus mykiss)", Vet. Immunol. Immunopathol, 140, pp.119~129 (2011)
- Rouxel, R. N., "Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine", J. Virol, 90, pp.10857~10866 (2016)
- Wattanavijarn, W, "First International Conference on the Impact of Viral Diseases on the Development of Asian Countries", First International Conference on the Impact of Viral Diseases on the Development of Asian Countries (1986)