Use of G gene-deleted single-cycle viral hemorrhagic septicemia virus (VHSV) for delivery of nervous necrosis virus (NNV)-like particles
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Vaccines based on single-cycle viruses that are replication-incompetent due to knockout of replication-related structural gene(s) are more immunogenic than inactivated or subunit vaccines and canbe used as delivery vehicles for foreign antigens without concerns on the reverting to virulent forms. The aim of this study was to develop a delivery vehicle for nervous necrosis virus (NNV)-like particles(VLPs) using G gene deleted single-cycle VHSV (rVHSV-ΔG). Recombinant single-cycle VHSVscarrying NNV capsid protein gene between N and P gene of rVHSV-ΔG genome (rVHSV-ΔG-NNVCap)were rescued by reverse genetic technology. The successful expression of NNV capsid protein incells infected with rVHSV-ΔG-NNVCap was demonstrated by Western blot analysis, and the productionof NNV VLPs in infected cells was confirmed using an electron microscopy. The results suggestthat single-cycle VHSVs can be used as a safe delivery vehicle for NNV VLPs, and can be extendedto other pathogens for the development of prophylactic vaccines.
Abstract
Vaccines based on single-cycle viruses that are replication-incompetent due to knockout of replication-related structural gene(s) are more immunogenic than inactivated or subunit vaccines and canbe used as delivery vehicles for foreign antigens without concerns on the reverting to virulent forms. The aim of this study was to develop a delivery vehicle for nervous necrosis virus (NNV)-like particles(VLPs) using G gene deleted single-cycle VHSV (rVHSV-ΔG). Recombinant single-cycle VHSVscarrying NNV capsid protein gene between N and P gene of rVHSV-ΔG genome (rVHSV-ΔG-NNVCap)were rescued by reverse genetic technology. The successful expression of NNV capsid protein incells infected with rVHSV-ΔG-NNVCap was demonstrated by Western blot analysis, and the productionof NNV VLPs in infected cells was confirmed using an electron microscopy. The results suggestthat single-cycle VHSVs can be used as a safe delivery vehicle for NNV VLPs, and can be extendedto other pathogens for the development of prophylactic vaccines.
참고문헌 (23)
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