바리과 어류 3종(능성어 Hyporthodus septemfasciatus, 붉바리 Epinephelus akaara, 자바리 Epinephelus bruneus)에 대한 신경괴사증바이러스(Nervous Necrosis Virus, NNV)의 생백신 처리 효과
Efficacy of live NNV immersion vaccination in three grouper species (Seven band grouper Hyporthodus septemfasciatus, Red spotted grouper Epinephelus akaara, Long tooth grouper Epinephelus bruneus)
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, we investigated safety and efficacy of a low temperature immunization protocol with NNV in red spotted grouper, Epinephelus akaara and long tooth grouper, Epinephelus bruneus and seven band grouper, Hyporthodus septemfasciatus. Further, growth rate between immunized and naïve fish was evaluated during the experiment to check side effect of immunization. Three grouper species were immunized by immersion method with live NNV at 105.0 TCID50/mL at 16.5˚C for 30 min and reared for 120 days at natural sea water temperature. To evaluate growth rate, total length and wet weight was measured 7 times after immunization. Immunized three grouper species were challenged by intramuscular inoculation with NNV at 104.2 TCID50/100 µL/fish. Immunization at low temperature with live NNV did not show any clinical symptoms of infection, mortality and inhibition of growth. After challenge, cumulative mortality of naïve seven band grouper, red spotted grouper, long tooth grouper were 45, 10, 20 %, respectively. However no mortality was observed at immunized groupers. Thus, it was demonstrated that immunization at low temperature with live NNV are able to protect three different species of groupers without inhibition of growth.
Abstract
In this study, we investigated safety and efficacy of a low temperature immunization protocol with NNV in red spotted grouper, Epinephelus akaara and long tooth grouper, Epinephelus bruneus and seven band grouper, Hyporthodus septemfasciatus. Further, growth rate between immunized and naïve fish was evaluated during the experiment to check side effect of immunization. Three grouper species were immunized by immersion method with live NNV at 105.0 TCID50/mL at 16.5˚C for 30 min and reared for 120 days at natural sea water temperature. To evaluate growth rate, total length and wet weight was measured 7 times after immunization. Immunized three grouper species were challenged by intramuscular inoculation with NNV at 104.2 TCID50/100 µL/fish. Immunization at low temperature with live NNV did not show any clinical symptoms of infection, mortality and inhibition of growth. After challenge, cumulative mortality of naïve seven band grouper, red spotted grouper, long tooth grouper were 45, 10, 20 %, respectively. However no mortality was observed at immunized groupers. Thus, it was demonstrated that immunization at low temperature with live NNV are able to protect three different species of groupers without inhibition of growth.
참고문헌 (17)
- Damsgård B, "Effects of infectious pancreatic necrosis virus(IPNV)on appetite and growth in Atlantic salmon, Salmo salar L", Aquaculture, 163, pp.185~193 (1998)
- 홍창기, "호르몬 처리에 의한 능성어 Epinephelus septemfasciatus의 배란유도 효과", 수산해양교육연구, 27(4), pp.981~989 (2015), 한국수산해양교육학회
- Kai YH, "Vaccination of grouper broodfish (Epinephelus tukula) reduces the risk of vertical transmission by nervous necrosis virus", Vaccine, 28, pp.996~1001 (2010)
- 김춘섭, "능성어 양식장에서의 viral nervous necrosis (VNN) 발생양상", 한국어병학회지, 25(2), pp.111~116 (2012), 한국어병학회[DOI]
- 김시우, "신경괴사증바이러스(Nervous Necrosis Virus, NNV) 모니터링을 통한 무감염 능성어(Hyporthodus septemfasciatus)친어의 선발", 한국수산과학회지, 50(5), pp.527~533 (2017), 한국수산과학회[DOI]
- Kim SW, "Efficacy of live NNV immersion vaccine immunized at low temperature in sevenband grouper, Epinephelus septemfasciatus", Virus research, 292, pp.198227~ (2021)
- 김위식, "능성어(Epinephelus septemfasciatus) 양식장에서의 바이러스성신경괴사증(VNN) 예방대책", 한국수산과학회지, 48(4), pp.403~410 (2015), 한국수산과학회
- Liu X, "A live attenuated Vibrio anguillarum vaccine induces efficient immunoprotection in Tiger puffer (Takifugurubripes)", Vaccine, 36, pp.1460~1466 (2018)
- Midtlyng PJ, "Growth of Atlantic salmon Salmo salar after intraperitoneal administration of vaccines containing adjuvants", Dis Aquat Org, 32, pp.91~97 (1998)
- Munday BL, "Betanoda virus infections of teleost fish : a review", J Fish Dis, 25, pp.127~142 (2002)
- Nguyen HD, "Progression of striped jack nervous necrosis virus (SJNNV) infection in naturally and experimentally infected striped jack Pseudocaranxdentex larvae", Dis Aquat Organ, 24, pp.99~105 (1996)
- Nishizawa T, "Potentiality of a live vaccine with nervous necrosis virus(NNV) for sevenband grouper Epinephelus septemfasciatus at a low rearing temperature", Vaccine, 30, pp.1056~1063 (2012)
- Nopadon P, "Nodavirus associated with pathological changes in adult spotted coralgroupers (Plectropomusmaculatus) in Thailand with viral nervous necrosis", Res Vet Sci, 87, pp.97~101 (2009)
- Oh MJ, "Comparison of the coat protein gene of nervous necrosis virus (NNV) detected from marine fishes in Korea", J World Aquac Soc, 36, pp.223~227 (2005)
- Oh MJ, "Assessment of the sevenband grouper Epinephelus septemfasciatus with a live nervous necrosis virus (NNV) vaccine at natural seawater temperature", Vaccine, 31, pp.2025~2027 (2013)
- Sohn SG, "Studies on the mass mortality of the cultured grouper, Epinephelus septemfasciatus", J Fish Pathol, 4, pp.87~94 (1991)
- Watanabe KI, "Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus", Dis Aquat Organ, 41, pp.219~223 (2000)