본문 바로가기
KERD
논문KCI 등재

순환여과시스템에서 양식되는 바리과 어류 3종[능성어, (Hyporthodus septemfasciatus), 자바리(Epinephelus moara), 교잡종 대왕자바리(E. moara ♀× E. lanceolatus ♂)]의 감염성 질병 모니터링

Monitoring of infectious diseases in three grouper species (Hyporthodus septemfasciatus, Epinephelus moara and E. moara ♀ × E. lanceolatus ♂ ) cultured in recirculating aquaculture systems

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.

Abstract

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.

참고문헌 (47)

  1. Adel, M., "Phylogenetic relationships of Iranian infectious hematopoietic necrosis virus of rainbow trout (Oncorhynchus mykiss)based on the glycoprotein gene", Archives of virology, 161, pp.657~663 (2016)[DOI]
  2. Bandín, Isabel, "Betanodavirus and VER disease: a 30-year research review", Pathogens, 9, pp.106~ (2020)[DOI]
  3. Boo, M. S., "Management and development of seed rearing in red spotted grouper Epinephelus akaara", Korean journal of fisheries and aquatic sciences, 53, pp.506~514 (2020)[DOI]
  4. "A guide to recirculation aquaculture" (2022)[URL]
  5. Bunlipatanon, P., "Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper (Epinephelus lanceolatus×Epinephelus fuscoguttatus)", Aquaculture research, 48, pp.1711~1723 (2017)[DOI]
  6. Cheng, A. -C., "Effects of temperature change on the innate cellular and humoral immune responses of orange-spotted grouper Epinephelus coioides and its susceptibility to Vibrio alginolyticus", Fish & shellfish immunology, 26, pp.768~772 (2009)[DOI]
  7. Cho, M. -Y., "Statistical data on fish virus of cultured olive flounder, Paralichthys olivaceus from 2005 to 2007", Journal of fish pathology, 23, pp.155~163 (2010)
  8. Chun, J., "Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus", Applied and environmental microbiology, 65, pp.2202~2208 (1999)[DOI]
  9. Colwell, R., "Polyphasic taxonomy of the genus Vibrio:numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species", Journal of bacteriology, 104, pp.410~433 (1970)[DOI]
  10. Dixon, P., "Epidemiological characteristics of infectious hematopoietic necrosis virus (IHNV): a review", Veterinary research, 47, pp.1~26 (2016)[DOI]
  11. FishStatJ, "A tool for fishery statistics analysis, FAO Fisheries and aquaculture department", FIPS-Statistics and information, ~ (2017)
  12. Harikrishnan, R., "Fish health aspects in grouper aquaculture", Aquaculture, 320, pp.1~21 (2011)[DOI]
  13. Harikrishnan, R., "Immunomodulatory effects of chitin and chitosan enriched diets in Epinephelus bruneus against Vibrio alginolyticus infection", Aquaculture, 326, pp.46~52 (2012)[DOI]
  14. Hassan, A., "Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis", Journal of clinical microbiology, 39, pp.1618~1621 (2001)[DOI]
  15. Hawley, L. M., "Stability of viral hemorrhagic septicemia virus (VHSV) in freshwater and seawater at various temperatures", Diseases of aquatic organisms, 82, pp.171~178 (2008)[DOI]
  16. Hoihuan, A., "Molecular genotyping and phenotyping of Vibrio vulnificus isolated from diseased, brown-marbled grouper (Epinephelus fuscoguttatus) in Thailand with preliminary vaccine efficacy analysis", Aquaculture, 545, pp.737188~ (2021)[DOI]
  17. Hu, Y. -q., "Rapid and simultaneous detection of viable Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae by PMAmPCR assay in aquatic products", LWT, 180, pp.114663~ (2023)[DOI]
  18. Huang, H. -Y., "Efficacy of a formalin-inactivated vaccine against Streptococcus iniae infection in the farmed grouper Epinephelus coioides by intraperitoneal immunization", Vaccine, 32, pp.7014~7020 (2014)[DOI]
  19. Huang, X., "Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China", Journal of fish diseases, 38, pp.379~387 (2015)[DOI]
  20. Hwang, J. Y., "Naturally occurring substitution in one amino acid in VHSV phosphoprotein enhances viral virulence in flounder", PLoS pathogens, 17(1), pp.e1009213~ (2021)[DOI]
  21. Jang, M. H., "Status and characteristics of JEECV (Japanese eel endothelial cell-infecting virus) and AnHV (Anguillid herpesvirus 1) infections in domestic farmed eels Anguilla japonica, Anguilla bicolor and Anguilla marmorata", Korean journal of fisheries and aquatic Sciences, 54, pp.668~675 (2021)[DOI]
  22. Kang, M. J., "The Embryonic Development and Hatchability of Two Hybrids with Giant Grouper Female: Giant Grouper♀× Kelp Grouper♂ and Giant Grouper♀× Red-Spotted Grouper♂", Korean journal of fisheries and aquatic sciences, 53, pp.557~562 (2020)[DOI]
  23. Kim, K. -R., "Effects of salinity, water temperature and development stage on the hatching rate and survival of fertilized eggs in hybrid grouper (Epinephelus fuscoguttatus♀× E. lanceolatus♂) for long-distance transport", Ocean and polar research, 40, pp.161~167 (2018)[DOI]
  24. Kim, Y. B., "Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene", Journal of clinical microbiology, 37, pp.1173~1177 (1999)[DOI]
  25. Kim, Y. H., "Molecular Biological Species Identification of Imported Groupers (Epinephelus moara♀× E. lanceolatus♂)", Korean journal of fisheries and aquatic sciences, 53, pp.566~571 (2020)[DOI]
  26. Lindholm‐Lehto, P, "Water quality monitoring in recirculating aquaculture systems", Aquaculture, fish and fisheries, 3, pp.113~131 (2023)[DOI]
  27. Ma, H., "Epidemiological characterization of VNNV in hatchery-reared and wild marine fish on Hainan Island, China, and experimental infection of golden pompano (Trachinotus ovatus) juveniles", Archives of virology, 160, pp.2979~2989 (2015)[DOI]
  28. Ma, H., "Co-infection of megalocytivirus and viral nervous necrosis virus in a very severe mass mortality of juvenile orange-spotted groupers (Epinephelus coioides)", Aquaculture, 358, pp.170~175 (2012)[DOI]
  29. Madsen, H. C., "Trichodina sp.(Ciliophora: Peritrichida) in eel Anguilla anguilla in recirculation systems in Denmark:host-parasite relations", Diseases of aquatic organisms, 42, pp.149~152 (2000)[DOI]
  30. Martins, C., "New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability", Aquacultural engineering, 43, pp.83~93 (2010)[DOI]
  31. Megarajan, S., "Molecular detection of betanodavirus in orange-spotted grouper (Epinephelus coioides) broodstock maintained in recirculating aquaculture systems and sea cages", Aquaculture international, 28, pp.349~362 (2020)[DOI]
  32. Natnan, M. E., "The Effect of Oleic Acid-Enriched Diet in Hybrid Groupers (Epinephelus fuscoguttatus× Epinephelus lanceolatus) upon Infection with Vibrio vulnificus Using an LC-qTOFMS Approach", Journal of marine science and engineering, 11, pp.1563~ (2023)[DOI]
  33. Nhung, P. H., "The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species", Systematic and applied microbiology, 30, pp.309~315 (2007)[DOI]
  34. Nishizawa, T., "Polymerase chain reaction(PCR)amplification of RNA of striped jack nervous necrosis virus(SJNNV)", Diseases of aquatic organisms, 18, pp.103~107 (1994)
  35. Panicker, G., "Rapid detection of Vibrio vulnificus in shellfish and Gulf of Mexico water by real-time PCR", Applied and environmental microbiology, 70, pp.498~507 (2004)[DOI]
  36. Park, C.-K., "The Mass Production of Fertilized Eggs for Industrial Aquaculture of the Convict Grouper Hyporthodus septemfasicatus", Korean journal of fisheries and aquatic sciences, 54, pp.31~37 (2021)[DOI]
  37. Park, J. Y., "Comparison of Morphological Characteristics and Its Parent Species Hybrid Grouper between a Epinephelus moara♀ and E. lanceolatus♂", Korean journal of fisheries and aquatic sciences, 53, pp.572~576 (2020)[DOI]
  38. Ranjan, R., "Conditioning, maturation and year‐round natural spawning of orangespotted grouper, Epinephelus coioides (Hamilton, 1822), in recirculating aquaculture system", Aquaculture research, 48, pp.5864~5873 (2017)[DOI]
  39. Rimmer, M. A., "A review of grouper (Family Serranidae: Subfamily Epinephelinae) aquaculture from a sustainability science perspective", Reviews in aquaculture, 11, pp.58~87 (2019)[DOI]
  40. Roach, J. C. M., "Identification of Streptococcus iniae by commercial bacterial identification systems", Journal of microbiological methods, 67, pp.20~26 (2006)[DOI]
  41. Sah Putra, B., "Prevalence of infectious spleen and kidney necrosis virus (ISKNV), nervous necrosis virus (NNV)and ectoparasites in juvenile Epinephelus spp. farmed in Aceh, Indonesia", pathogens, 9, pp.578~ (2020)[DOI]
  42. Shen, G., "Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus× Epinephelus lanceolatus", Journal of fish diseases, 40, pp.1351~1362 (2017)[DOI]
  43. Verma, D. K., "Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus", Journal of environmental biology, 36, pp.433~ (2015)
  44. Wang, L., "Transcriptome comparative analysis of immune tissues from asymptomatic and diseased Epinephelus moara naturally infected with nervous necrosis virus", Fish &shellfish immunology, 93, pp.99~107 (2019)[DOI]
  45. Won, K. M., "A case study of mortality caused by viral encephalopathy and retinopathy (VER) in cultured sevenband grouper, Epinephelus septemfasciatus during Winter", Korean journal of ichthyology, 29, pp.157~164 (2017)
  46. Yang, S. G., "Adaptive characteristics of the longtooth grouper Epinephelus bruneus according to stocking density and water temperature", Korean journal of fisheries and aquatic sciences, 47, pp.847~852 (2014)[DOI]
  47. Zorriehzahra, M. J., "Emerging and reemerging viral pathogens", pp.673~703 (2020)

관련 논문AI추천

이 간행물의 다른 논문 (16건)