여윔증상 넙치 및 사육수 내 검출된 점액포자충(Enteromyxum leei and Parvicapsula anisocaudata)의 정량적 분석
Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) detected from emaciated olive flounder (Paralichthys olivaceus) and rearing water
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Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata)were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine,spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 108 copies/mg tissue)of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 103~4.6 × 105copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata,it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 ×103 copies/mg tissue) and body kidney (9.1 × 103 copies/mg tissue). In order to investigate whethermyxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei andP. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNAcopy number of E. leei from rearing water of farm-A and farm-B were 8 × 104 and 5 × 105 copies/L,respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A,farm-B and farm-C showed 0, 2.0 × 106 and 5.1 × 106 copies/L, respectively.
Abstract
Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata)were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine,spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 108 copies/mg tissue)of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 103~4.6 × 105copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata,it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 ×103 copies/mg tissue) and body kidney (9.1 × 103 copies/mg tissue). In order to investigate whethermyxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei andP. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNAcopy number of E. leei from rearing water of farm-A and farm-B were 8 × 104 and 5 × 105 copies/L,respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A,farm-B and farm-C showed 0, 2.0 × 106 and 5.1 × 106 copies/L, respectively.
참고문헌 (22)
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