본문 바로가기
KERD
논문KCI 등재

Prevention of vibriosis in sea bass, Dicentrarchus labrax using ginger nanoparticles and Saccharomyces cerevisiae

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Vibriosis is an important septicemic bacterial disease that affects a variety of commercial fish species,including cultured Dicentrarchus labrax. Nanotechnology has become an important modern tool forfish diseases prevention. Furthermore, nanomaterials have the ability to prevent and treat fish diseases. The current study was aimed to identify the causative agent of massive mortality of D. labrax commercialfarm in Alexandria, Egypt. Experimental infection and the median lethal dose (LD50) of pathogenicisolate were assessed. Also, the effect of ginger nanoparticles (GNPs) and Sacchromyces cerevisiaeas feed additives for prevention of vibriosis in D. labrax was carried out. Similarly, the tissue immunstimulantgenes, IL‐1β and TLR2 were measured in the spleen of feeding groups. The clinical signsof naturally diseased D. labrax showed corneal opacity and paleness of gills with excessive mucoussecretion. The post-mortem abnormalities were severe hemorrhage and adhesion of internal organs. After bacteriological isolation and identification, the causative agent of mortality in the current studywas Vibrio alginolyticus. The LD50 of V. alginolyticus was 1.5×105.4 CFU/ml. The experimentallyinfected D. labrax showed ulceration, exophthalmia and skin hemorrhages. The post-mortem findingsof the experimentally infected D. labrax revealed internal hemorrhage, spleen darkness and palenessof liver. There is no mortality and 100% RPS in groups fed GNPs then injected with V. alginolyticus,in those fed a combination of GNPs and S. cerevisiae and a group fed normal diet then injected withphysiological saline (control negative), respectively. Contrarily, there was 10% mortality and 87.5RPS in the group fed S. cerevisae then injected with V. alginolyticus. On the other hand, the controlpositive group showed 79% mortality. The spleen IL‐1β and TLR2 immunostimulant genes weresignificantly increased in groups of fish fed GNNP, S. cerevisiae and a combination of GNPs andS. cerevisiae, respectively compared to control group. The highest stimulation of those immunostimulantgenes was found in the group fed a combination of GNPs and S. cerevisiae, while fish fed S. cerevisiae had the lowest level. Dietary combination of GNPs and S. cerevisiae was shown to beefficient in preventing of vibriosis, with greatest stimulation of spleen IL‐1β and TLR2 immunostimulantgenes.

Abstract

Vibriosis is an important septicemic bacterial disease that affects a variety of commercial fish species,including cultured Dicentrarchus labrax. Nanotechnology has become an important modern tool forfish diseases prevention. Furthermore, nanomaterials have the ability to prevent and treat fish diseases. The current study was aimed to identify the causative agent of massive mortality of D. labrax commercialfarm in Alexandria, Egypt. Experimental infection and the median lethal dose (LD50) of pathogenicisolate were assessed. Also, the effect of ginger nanoparticles (GNPs) and Sacchromyces cerevisiaeas feed additives for prevention of vibriosis in D. labrax was carried out. Similarly, the tissue immunstimulantgenes, IL‐1β and TLR2 were measured in the spleen of feeding groups. The clinical signsof naturally diseased D. labrax showed corneal opacity and paleness of gills with excessive mucoussecretion. The post-mortem abnormalities were severe hemorrhage and adhesion of internal organs. After bacteriological isolation and identification, the causative agent of mortality in the current studywas Vibrio alginolyticus. The LD50 of V. alginolyticus was 1.5×105.4 CFU/ml. The experimentallyinfected D. labrax showed ulceration, exophthalmia and skin hemorrhages. The post-mortem findingsof the experimentally infected D. labrax revealed internal hemorrhage, spleen darkness and palenessof liver. There is no mortality and 100% RPS in groups fed GNPs then injected with V. alginolyticus,in those fed a combination of GNPs and S. cerevisiae and a group fed normal diet then injected withphysiological saline (control negative), respectively. Contrarily, there was 10% mortality and 87.5RPS in the group fed S. cerevisae then injected with V. alginolyticus. On the other hand, the controlpositive group showed 79% mortality. The spleen IL‐1β and TLR2 immunostimulant genes weresignificantly increased in groups of fish fed GNNP, S. cerevisiae and a combination of GNPs andS. cerevisiae, respectively compared to control group. The highest stimulation of those immunostimulantgenes was found in the group fed a combination of GNPs and S. cerevisiae, while fish fed S. cerevisiae had the lowest level. Dietary combination of GNPs and S. cerevisiae was shown to beefficient in preventing of vibriosis, with greatest stimulation of spleen IL‐1β and TLR2 immunostimulantgenes.

참고문헌 (58)

  1. Abass, DA., "Dietary supplementation of yeast (Saccharomyces cerevisiae) improves growth, stress tolerance, and disease resistance in juvenile Nile tilapia (Oreochromis niloticus)", Aquacult Int, 26, pp.843~855 (2018)
  2. Abdelaziz M., "Monitoring of different vibrio species affecting marine fishes in Lake Qarun and Gulf of Suez: Phenotypic and molecular characterization", Egyptian Journal of Aquatic Research, 43, pp.141~146 (2017)[DOI]
  3. Abdel-Aziz, M., "Identifying some pathogenic Vibrio", Photobacterium species during mass mortality of cultured Gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax) from some Egyptian coastal provinces, 2013, pp.1, 87~95
  4. Mohsen Abdel-Tawwab, "Evaluation of commercial live bakers’ yeast, Saccharomyces cerevisiae as a growth and immunity promoter for Fry Nile tilapia, Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila", Aquaculture, 280(1-4), pp.185~189 (2008), Elsevier BV[DOI]
  5. Amend, D. F., "Potency testing of fish vaccines. International symposium on fish Biologics: Serodiagnostics and Vaccines", Developmental biology, 49, pp.447~454 (1981)
  6. SERGIO A ARANCIBIA, "Toll-like Receptors are Key Participants in Innate Immune Responses", Biological Research, 40(2), pp.97~112 (2007), SciELO Agencia Nacional de Investigacion y Desarrollo (ANID)[DOI]
  7. Austin, B., "Bacterial fish pathogens: diseases of farmed and wild fish", pp.2012, Springer
  8. Basniwal, R. K., "Curcumin nanoparticles: preparation, characterization and antimicrobial studies", Journal of Agricultural Food Chemistry, 59, pp.2056~2061 (2011)[DOI]
  9. Bird, S., "Characterisation and expression analysis of interleukin 2 (IL-2) and IL-21 homologues in the Japanese pufferfish, Fugu rubripes, following their discovery by synteny", Immunogenetics, 56(12), pp.909~923 (2005)[DOI]
  10. Castro, R., "Cortisol modulates the induction of inflammatory gene expression in a rainbow trout macrophage cell line", Fish & Shellfish Immunology, 30, pp.215~223 (2011)[DOI]
  11. Cebeci , A., "Prediction of Toll-Like Receptor Gene Family in Asian Sea Bass", Genetics of Aquatic Organisms, 3(2), pp.79~91 (2019)[DOI]
  12. Chiu C-H, "Dietary administration of the probiotic, Saccharomyces cerevisiae P13, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides", Fish Shellfish Immunol, 29, pp.1053~1059 (2010)[DOI]
  13. Chistiakov,D. A., "A variant of the interleukin-1beta gene in European sea bass, Dicentrarchus labrax L., is associated with increased resistance against Vibrio anguillarum", Journal of Fish Diseases, 33(9), pp.759~767 (2010)[DOI]
  14. Cuesta A., "Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.)", Vet Immunol Immunopathol, 101, pp.203~210 (2004)[DOI]
  15. Defoirdt, T., "Alternatives to antibiotics to control bacterial infections: luminescent vibriosis in aquaculture as an example", Trends in Biotechnology, 25(10), pp.472~479 (2007)[DOI]
  16. Esteban MA, "Glucan receptor but not mannose receptor is involved in the phagocytosis of Saccharomyces cerevisiae by seabream (Sparus aurata L.) blood leucocytes", Fish & Shellfish Immunology, 16(3), pp.447~451 (2004), Elsevier BV[DOI]
  17. Gargouti A. S., "Detection of tdh and trh Toxic Genes in Vibrio Alginolyticus Strain from Mantis Shrimp (Oratosquilla Oratoria)", J Nutr Food Sci, 5, pp.405~ (2015)[DOI]
  18. Govindaraju, K., "Application of Nanotechnology in Diagnosis and Disease Management of White Spot Syndrome Virus (WSSV) in Aquaculture", J. Clust. Sci., 31, pp.1163~1171 (2020)
  19. Grisez, L., "(Listonella) anguillarum infections in marine fish larviculture", Fish and crustacean larviculture symposium (1995), European Aquaculture Society
  20. Gröner, F., "Effects of the pharmaceuticals diclofenac and metoprolol on gene expression levels of enzymes of biotransformation, excretion pathways and estrogenicity in primary hepatocytes of Nile tilapia (Oreochromis niloticus)", Comparative Biochemistry and Physiology, 167, pp.51~57 (2015)[DOI]
  21. Harikrishnan R, "Immunomodulatory effect of probiotics enriched diets on Uronema marinum infected olive flounder", Fish Shellfish Immunol, 30, pp.964~971 (2011)[DOI]
  22. Hayatgheib, N., "A review of functional feeds and the control of Aeromonas infections in freshwater fish", Aquacult Int, 28, pp.1083~1123 (2020)[DOI]
  23. Heng, J., "The polymorphism and haplotype of TLR3 gene in grass carp (Ctenopharyngodon idella) and their associations with susceptibility", resistance to grass carp reovirus, 2011, pp.30(1), 45~50
  24. Holt, J. G., "Bergey’s Manual of Determinative Bacteriology" (1994), Williams &Wilkins
  25. Hoseinifar, S. H., "Probiotic, prebiotic and synbiotic supplements in sturgeon aquaculture: a review", Rev. Aquacult., 8, pp.89~102 (2016)[DOI]
  26. Irianto, A., "Probiotics in aquaculture", Fish Shellfish Immun, 25, pp.633~642 (2002)[DOI]
  27. Khosravi-Katuli, K., "Effects of nanoparticles in species of aquaculture interest", Environ Sci Pollut Res, 24, pp.17326~17346 (2017)[DOI]
  28. Kim D. H., "Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics", Fish Shellfish Immunol, 21, pp.513~524 (2006)[DOI]
  29. Korni, F., "Effect of ginger and its nanoparticles on growth performance, cognition capability, immunity and prevention of motile Aeromonas septicaemia in Cyprinus carpio fingerlings", Aquaculture Nutrition, 23 (2017)[DOI]
  30. Fatma M.M. Korni, "Prevention of Edwardsiellosis in Clarias gariepinus using ginger and its nanoparticles with a reference to histopathological alterations", Aquaculture, 539, pp.736603~ (2021), Elsevier BV[DOI]
  31. Jale Korun, "MARINE VIBRIOS ASSOCIATED WITH DISEASED SEA BASS (Dicentrarchus labrax) IN TURKEY", Journal of Fisheries Sciences, 2(1), pp.66~76 (2008), Scitechnol Biosoft Pvt. Ltd.[DOI]
  32. Peng Li, "Nucleotide nutrition in fish: Current knowledge and future applications", Aquaculture, 251(2-4), pp.141~152 (2006), Elsevier BV[DOI]
  33. Li Q, "Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications", Water Res, 42, pp.4591~4602 (2008)[DOI]
  34. Liu P. C., "Isolation and characterization of pathogenic Vibrio alginolyticus from diseased cobia Rachycentron canadum", Journal of Basic Microbiology, 44, pp.23~28 (2004)[DOI]
  35. Jep Lokesh, "Transcriptional regulation of cytokines in the intestine of Atlantic cod fed yeast derived mannan oligosaccharide or β-Glucan and challenged with Vibrio anguillarum", Fish & Shellfish Immunology, 33(3), pp.626~631 (2012), Elsevier BV[DOI]
  36. Gloria López-Castejón, "The type II interleukin-1 receptor (IL-1RII) of the bony fish gilthead seabream Sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels", Molecular Immunology, 44(10), pp.2772~2780 (2007), Elsevier BV[DOI]
  37. Mustapha, S., "Vibrio Alginolyticus:An Emerging Pathogen of Foodborne Diseases", International Journal of Science and Technology, 2(4) (2013)
  38. Norhidayah, A., "Assessment of antimicrobial activity of nanoparticle ginger rhizome water extract", International Journal of Scientific and Research Publications, 5(11), pp.656~669 (2015)
  39. Ortuño J, "Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (Sparus aurata)", Vet Immunol Immunopathol, 85, pp.41~50 (2002)[DOI]
  40. Gustavo Pinoargote, "Evaluation of the Efficacy of Probiotics in vitro Against Vibrio parahaemolyticus, Causative Agent of Acute Hepatopancreatic Necrosis Disease in Shrimp", Journal of Probiotics & Health, 6(01), pp.49~89 (2018), OMICS Publishing Group[DOI]
  41. Pridgeon, J. W., "Major bacterial diseases in aquaculture and their vaccine development", CAB Reviews, 7, pp.1~16 (2012)[DOI]
  42. Rattanachaikunsopon, P., "Assessment of synergistic efficacy of carvacrol and cymene against Edwardsiella tarda in vitro and in Tilapia (Oreochromis niloticus)", African Journal of Microbiology Research, 4, pp.420~425 (2010)
  43. Sajeevan T. P., "Dose", frequency:a critical factor in the administration of glucan as immunostimulant to Indian white shrimp Fenneropenaeus indicus, 2009, pp.287, 248~252
  44. Rodríguez A., "Immunostimulant properties of a cell wallmodified whole Saccharomyces cerevisiae strain administered by diet to seabream (Sparus aurata L.)", Vet Immunol Immunopathol, 96, pp.183~192 (2003)[DOI]
  45. Saifuddin, N., "Rapid Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Bacteria with Microwave Irradiation", E-Journal of Chemistry, 6(1), pp.61~70 (2009)[DOI]
  46. Sayes, C., "Antibiotic Use in Animals", pp.115~132 (2018), Intech Open
  47. Mohamed Shaalan, "Recent progress in applications of nanoparticles in fish medicine: A review", Nanomedicine: Nanotechnology, Biology and Medicine, 12(3), pp.701~710 (2016), Elsevier BV[DOI]
  48. Sharma S. R. K., "Vibrio alginolyticus infection in Asian seabass (Lates calcarifer, Bloch)reared in open sea floating cages in India", Aquaculture Research, 44, pp.86~92 (2013)[DOI]
  49. Sims, J. E., "The IL-1 family: regulators of immunity", Nat Rev Immunol, pp.89~102 (2010)[DOI]
  50. Sugita H, "The vitamin B12-producing ability of intestinal bacteria isolated from tilapia and channel catfish", Nippon Suisan Gakkaishi, 56, pp.701~ (1990)
  51. Laszlo Takacs, "Self-sustaining reactions induced by ball milling", Progress in Materials Science, 47(4), pp.355~414 (2002), Elsevier BV[DOI]
  52. Thune R.L., "Pathogenesis of gram-negative bacterial infections in warm water fish", Annual Review of Fish Diseases, 3, pp.37~68 (1993)[DOI]
  53. Toranzo, A.E., "Mediterranean Aquaculture Diagnostic Laboratories" (2004), CIHEAM
  54. Toranzo, A. E., "A review of the main bacterial fish diseases in mariculture systems", Aquaculture, 246, pp.37~61 (2005)[DOI]
  55. Tukmechi A., "Effects of Saccharomyces cerevisiae supplementation on immune response, hematological parameters, body composition and disease resistance in rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)", Journal of Applied Ichthyology, 30, pp.55~61 (2014)[DOI]
  56. S. Varsamos, "Husbandry stress during early life stages affects the stress response and health status of juvenile sea bass, Dicentrarchus labrax", Fish & Shellfish Immunology, 20(1), pp.83~96 (2006), Elsevier BV[DOI]
  57. Yuan, J.S., "Statistical analysis of real-time PCR data", BMC Bioinformatics, 7, pp.85~ (2006)[DOI]
  58. I Zorrilla, "Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products", Journal of Fish Diseases, 26(2), pp.103~108 (2003), Wiley[DOI]

관련 논문AI추천

Prevention of Citrobacter freundii (MW279218) infection in Nile tilapia, Oreochromis niloticus using zinc oxide nanoparticlesAsmaa Edrees, Asmaa N. Mohammed, Fatma M. M. Korni, Usama K. Moawad수산생명의학회 · 한국어병학회지2022 · 제35권 제1호Efficacy of dietary propolis and its nanoparticles on immune- response, stress indicators, and prevention of Pseudomonas aeruginosa infection in Oreochromis niloticusAhmed Farghali, Asmaa N. Mohammed, Fatma M. M. Korni, Nour El-Houda y. Hassan, Rehab K. Mahmoud, Usama K. Moawad수산생명의학회 · 한국어병학회지2023 · 제36권 제1호Effects of lactobacillus fermented brewer's yeast by-products on growth performance, innate immunity and antibacterial activity in Carp, Cyprinus carpio김재훈, 양은총, 이찬흔, 장태원, 정상목, 정해인, 최상훈, 최재혁, 황유진수산생명의학회 · 한국어병학회지2023 · 제36권 제2호The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus강인성, 강태윤, 박관하, 방석진, 전세영, 정상목, 최상훈, 최재혁, 황요셉수산생명의학회 · 한국어병학회지2019 · 제32권 제1호Dietary inclusion effect of yacon, ginger, and blueberry on growth, body composition, and disease resistance of juvenile black rockfish (Sebastes schlegeli) against Vibrio anguillarum정해승, 조성환, Lee Ki Wook한국수산과학회 · Fisheries and Aquatic Sciences2020 · 제23권 제2호Identification of the main cause of mortality in a commercial Oreaochromis niloticus farm: The role of poly-β-hydroxybutyrate as a preventive measure against EdwardsiellosisFatma I. Abo El-Ela, Fatma M. M. Korni, Usama K. Moawad수산생명의학회 · 한국어병학회지2023 · 제36권 제1호

이 간행물의 다른 논문 (15건)