The immune-adjuvant effect and safety of recombinant CC chemokine 1 (rRbCC1) in rock bream, Oplegnathus fasciatus
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.
Abstract
Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.
참고문헌 (33)
- Aakre, R., "Enhanced antibody response in Atlantic salmon (Salmo salar L.) to Aeromonas salmonicida cell wall antigens using a bacterin containing β- 1,3-M-glucan as adjuvant", Fish Shellfish Immunol, 4, pp.47~61 (1994)
- Agnew, W., "Streptococcus iniae : an aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination", Vet. Microbiol, 122, pp.1~15 (2007)
- Altman, A., "Immunomodifiers in vaccines", Adv. Vet. Sci. Comp. Med, 23, pp.301~341 (1989)
- Se Chang Park, "Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island", Journal of Veterinary Science, 7(1), pp.53~58 (2006), 대한수의학회
- Bercovier, H., "Immunization with bacterial antigens : infections with streptococci and related organisms", Dev. Biol. Stand, 90, pp.153~160 (1997)
- Berg, A., "Fish size at vaccination influence the development of side-effects in Atlantic salmon (Salmo salar L.)", Aquaculture, 265, pp.9~15 (2007)
- Cataldi, E., "Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces : Acipenseriformes) : effects of temperature and stress", Comp. Biochem. Physiol. Part A, 121, pp.351~354 (1998)
- 조미영, "넙치의 b-용혈성 연쇄구균 불활화백신의 안정성과 효능", 한국어병학회지, 19(2), pp.165~172 (2006), 한국어병학회
- Chu, W.H., "Adjuvant effect of propolis on immunisation by inactivated Aeromonas hydrophila in carp (Carassius auratus gibelio)", Fish Shellfish Immunol, 21, pp.113~117 (2006)
- Cox, J.C., "Adjuvants-a classification and review of their modes of action", Vaccine, 15, pp.248~256 (1997)
- Freund, J., "Sensitization and antibody formation after injection of tubercle bacilli and paraffin oil", Proc. Soc. Exp. Biol. Med, 37, pp.509~ (1937)
- Hastein, T., "Bacterial vaccines for fish--an update of the current situation worldwide", Dev. Biol (Basel), 121, pp.55~74 (2005)
- Horne, M.T., "The effects of the use of potassium alum adjuvant in vaccines against vibriosis in rainbow trout, Salmo gairdneri Richardson", J. Fish Dis, 7, pp.91~99 (1984)
- Jiao, X.D., "Comparative study of the effects of aluminum adjuvants and Freund’s incomplete adjuvant on the immune response to an Edwardsiella tarda major antigen", Vaccine, 28, pp.1832~1837 (2010)
- 정승희, "해상가두리 양식장 어류의 혈액 검사에 사용된 동물용 생화학 분석장치의 활용 가능성", 한국어병학회지, 19(3), pp.253~265 (2006), 한국어병학회
- Kim, J.W., "Molecular identification and expression analysis of the CC chemokine gene in rock bream (Oplegnathus fasciatus) and the biological activity of the recombinant protein", Fish Shellfish Immunol, 34, pp.892~901 (2013)
- Lindblad, E.B., "Aluminium compounds for use in vaccines", Immunol. Cell. Biol, 82, pp.497~505 (2004)
- Lofthouse, S.A., "Humoral and cellular responses induced by intradermally administered cytokine and conventional adjuvants", Vaccine, 13, pp.1131~1137 (1995)
- McElrath, M.J., "Selection of potent immunological adjuvants for vaccine construction", Semin. Cancer Biol, 6, pp.375~385 (1995)
- Mulvey, B., "Effects of potassium sulphate (alum) used in Aeromonas salmonicida bacterin on Atlantic salmon, Salmo salar L", J. Fish Dis, 18, pp.495~506 (1995)
- Mutoloki, S., "Sequential study of antigen persistence and concomitant inflammatory reactions relative to side-effects and growth of Atlantic salmon (Salmo salar L.) following intraperitoneal injection with oil-adjuvanted vaccines", Fish Shellfish Immunol, 16, pp.633~644 (2004)
- Mutoloki, S., "Time-course study of injection site inflammatory reactions following intraperitoneal injection of Atlantic cod (Gadus morhua L.) with oil-adjuvanted vaccines", Fish Shellfish Immunol, 24, pp.386~393 (2008)
- Park, S.I., "Disease control in Korean aquaculture", Fish Pathol, 44, pp.19~23 (2009)
- Poppe, T.T., "Pathology of Atlantic salmon Salmo salar intraperitoneally immunized with oil-adjuvanted vaccine. A case report", Dis. Aquat. Organ, 29, pp.219~226 (1997)
- Robertsen, B., "Enhancement of non-specific disease resistance in Atlantic salmon, Salmo salar L., by a glucan from Saccharomyces cerevisiae cell walls", J. Fish Dis, 13, pp.391~400 (1990)
- Rorstad, G., "Adjuvant furunculocis in Atlantic salmon (Salmo salar L.)", Fish Shellfish Immunol, 3, pp.179~190 (1993)
- Scheerlinck, J.Y., "Genetic adjuvants for DNA vaccines", Vaccine, 19, pp.2647~2656 (2001)
- Shi, X.J., "Immune enhancing effects of recombinant bovine IL-18 on foot-and-mouth disease vaccination in mice model", Vaccine, 25, pp.1257~1264 (2007)
- Shutou, K., "Role of capsule in immunogenicity of Streptococcus iniae to Japanese flounder Paralichthys olivaceus", Fish Pathol, 42, pp.101~106 (2007)
- Singh, M., "Advances in vaccine adjuvant", Nat. Biotechnol, 17, pp.1075~1081 (1999)
- Sommerset, I., "Vaccines for fish in aquaculture", Expert Rev. Vaccines, 4, pp.89~101 (2005)
- Sun, Y., "A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum", Fish Shellfish immunol, 31, pp.595~599 (2011)
- Williams, D.L., "Immunization against Trypanosoma cruzi : adjuvant effect of glucan", Int. J. Immunopharmacol, 11, pp.403~410 (1989)