Induction of antiviral mechanisms by interferon-related genes in rock bream (Oplegnathus fasciatus) infected with rock bream iridovirus (RBIV)
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
We evaluated the transcriptional response of interferon (IFN)-related genes in rock bream iridovirus (RBIV)-infected rock bream under high-, low-, or no-mortality conditions induced by different stocking water temperatures. Under the high susceptibility condition (group A, water temperature 26°C, 100% mortality), only the Mx gene was expressed early, with prolonged expression, and with heavy viral loads of approximately 106~107 major capsid protein gene copies/μL from 4 to 10 days post infection (dpi). However, IRF1, IRF3, IRF8, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56 were activated at later time points (8 dpi) and then quickly decreased (10 dpi). For the low susceptibility condition, the water temperature was set at 23°C for 7 days (group B) and then reduced to 17°C. Group B exhibited a 28% mortality rate, in which persistent and effective antiviral responses were observed for long periods of time. In particular, at 20 and 22 dpi, when virus replication was peaked at approximately 107/μL, the expressions of most of the IFN-related genes (IRF1, IRF3, IRF8, Mx, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56) were significantly higher in group B than in the control group. Moreover, prolonged and higher levels of IRF3 (at least 30 dpi), IRF8 (at least 30 dpi), ISG15 (at least 30 dpi), PKR (at least 28 dpi), Viperin (at least 30 dpi), and IFI44 (at least 30 dpi) were also observed in the recovery stage of infection. Under the no-susceptibility condition at 17°C (0% mortality), significantly elevated levels of IRF3, Mx, ISG15, and PKR were observed mostly until 20 dpi. The findings indicate that RBIV infection can induce an efficient IFN-mediated antiviral immune response in low- and no-susceptibility conditions. The findings could be valuable for effective control of viral pathogens in fish.
Abstract
We evaluated the transcriptional response of interferon (IFN)-related genes in rock bream iridovirus (RBIV)-infected rock bream under high-, low-, or no-mortality conditions induced by different stocking water temperatures. Under the high susceptibility condition (group A, water temperature 26°C, 100% mortality), only the Mx gene was expressed early, with prolonged expression, and with heavy viral loads of approximately 106~107 major capsid protein gene copies/μL from 4 to 10 days post infection (dpi). However, IRF1, IRF3, IRF8, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56 were activated at later time points (8 dpi) and then quickly decreased (10 dpi). For the low susceptibility condition, the water temperature was set at 23°C for 7 days (group B) and then reduced to 17°C. Group B exhibited a 28% mortality rate, in which persistent and effective antiviral responses were observed for long periods of time. In particular, at 20 and 22 dpi, when virus replication was peaked at approximately 107/μL, the expressions of most of the IFN-related genes (IRF1, IRF3, IRF8, Mx, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56) were significantly higher in group B than in the control group. Moreover, prolonged and higher levels of IRF3 (at least 30 dpi), IRF8 (at least 30 dpi), ISG15 (at least 30 dpi), PKR (at least 28 dpi), Viperin (at least 30 dpi), and IFI44 (at least 30 dpi) were also observed in the recovery stage of infection. Under the no-susceptibility condition at 17°C (0% mortality), significantly elevated levels of IRF3, Mx, ISG15, and PKR were observed mostly until 20 dpi. The findings indicate that RBIV infection can induce an efficient IFN-mediated antiviral immune response in low- and no-susceptibility conditions. The findings could be valuable for effective control of viral pathogens in fish.
참고문헌 (51)
- Jun Kurita, "Megalocytiviruses", Viruses, 4(4), pp.521~538 (2012), MDPI AG[DOI]
- S J Jung, "Iridovirus‐like infection associated with high mortalities of striped beakperch, <i>Oplegnathus fasciatus<", i> (Temminck et Schlegel), in southern coastal areas of the Korean peninsula, 2000, pp.23(3), 223~226[DOI]
- Jung MH, "Effects of water temperature on mortality in Megalocytivirus-infected rock bream Opleg- nathus fasciatus (Temminck et Schlegel) and devel- opment of protective immunity", J Fish Dis, 38, pp.729~737 (2015)[DOI]
- Jung MH, "Low pathogenicity of FLIV (flounder iridovirus) and the absence of cross-protection between FLIV and RBIV (rock bream irido- virus)", J Fish Dis, 39, pp.1325~1333 (2016)[DOI]
- Jung MH, "Protective immunity against Megalocytivirus infection in rock bream (Oplegnathus fasciatus) following CpG ODN administration", Vaccine, 35, pp.3691~ (2017)[DOI]
- Myung-Hwa Jung, "Protective immunity against rock bream iridovirus (RBIV) infection and TLR3-mediated type I interferon signaling pathway in rock bream ( Oplegnathus fasciatus ) following poly (I:C) administration", Fish & Shellfish Immunology, 67, pp.293~301 (2017), Elsevier BV[DOI]
- Myung-Hwa Jung, "Rock bream iridovirus (RBIV) replication in rock bream (Oplegnathus fasciatus) exposed for different time periods to susceptible water temperatures", Fish & Shellfish Immunology, 70, pp.731~735 (2017), Elsevier BV[DOI]
- Yi-Bing Zhang, "Molecular regulation of interferon antiviral response in fish", Developmental & Comparative Immunology, 38(2), pp.193~202 (2012), Elsevier BV[DOI]
- Bergan V, "Regulation and function of interferon regulatory factors of Atlantic salmon", Mol Immunol, 47, pp.2005~2014 (2010)[DOI]
- Mamane Y, "Interferon regulatory factors: the next generation", Gene, 237, pp.1~14 (1999)[DOI]
- Yasuike M, "Identification and characterization of Japanese flounder, Paralichthys olivaceus interferon-stimulated gene 15 (JfISG15)", Comp Immunol Microb, 34, pp.83~89 (2011)[DOI]
- Lee JY, "Cloning and analysis of ex- pression of Mx cDNA in Japanese flounder, Parali- chthys olivaceus", Dev Comp Immunol, 24, pp.407~415 (2000)[DOI]
- Samuel CE, "Antiviral actions of interferons", Clin Micro- biol Rev, 14, pp.778~809 (2001)[DOI]
- Hallen LC, "Antiproliferative activity of the human IFN-α-inducible protein IFI44", J Interferon Cytokine Res, 27, pp.675~680 (2007)[DOI]
- Arunachalam B, "Enzymatic reduction of disulfide bonds in lyso- somes: characterization of a gamma-interferon-in- ducible lysosomal thiol reductase (GILT)", Proc Natl Acad Sci, 97, pp.745~750 (2000)[DOI]
- Landolfo S, "Mechanisms of viral inhibition by interferons", Phar- macol Therapeut, 65, pp.415~442 (1995)[DOI]
- Chen YM, "Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterisation of its ex- pression in response to nodavirus", Fish Shellfish Immunol, 20, pp.58~71 (2006)[DOI]
- Rannveig Larsen, "Inhibition of Infectious Pancreatic Necrosis Virus Replication by Atlantic Salmon Mx1 Protein", Journal of Virology, 78(15), pp.7938~7944 (2004), American Society for Microbiology[DOI]
- Chih-Hung Lin, "Inhibition of nervous necrosis virus propagation by fish Mx proteins", Biochemical and Biophysical Research Communications, 351(2), pp.534~539 (2006), Elsevier BV[DOI]
- Christopher Marlowe A Caipang, "In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx", Virology, 317(2), pp.373~382 (2003), Elsevier BV[DOI]
- Avunje S, "Toll-like receptors and interferon associated immune factors in viral haemorrhagic septicaemia virus-infected olive flounder (Paralichthys olivaceus)", Fish Shellfish Immunol, 31, pp.407~414 (2011)[DOI]
- Thanasaksiri K, "Influence of temperature on Mx gene ex- pression profiles and the protection of sevenband grouper, Epinephelus septemfasciatus, against redspotted grouper nervous necrosis virus (RGNNV) infection after poly (I:C) injection", Fish Shellfish Immunol, 40, pp.441~445 (2014)[DOI]
- Jialin Zhang, "The influence of temperature on viral replication and antiviral-related genes response in hirame rhabdovirus-infected flounder (Paralichthys olivaceus)", Fish & Shellfish Immunology, 68, pp.260~265 (2017), Elsevier BV[DOI]
- Myung-Hwa Jung, "Gene expression regulation of the TLR9 and MyD88-dependent pathways in rock bream against rock bream iridovirus (RBIV) infection", Fish & Shellfish Immunology, 70, pp.507~514 (2017), Elsevier BV[DOI]
- Myung-Hwa Jung, "Gene expression of pro- and anti-apoptotic proteins in rock bream (Oplegnathus fasciatus) infected with megalocytivirus (family Iridoviridae)", Fish & Shellfish Immunology, 37(1), pp.122~130 (2014), Elsevier BV[DOI]
- Chamilani Nikapitiya, "Identification and Molecular Characterization of Z", ZE Lineage MHC Class I Heavy Chain Homologue and ^|^beta;2-microglobulin from Rock Bream Oplegnathus fasciatus, 2014, pp.49(3), 93~112[DOI]
- Myung-Hwa Jung, "CpG ODN 1668 induce innate and adaptive immune responses in rock bream ( Oplegnathus fasciatus ) against rock bream iridovirus (RBIV) infection", Fish & Shellfish Immunology, 69, pp.247~257 (2017), Elsevier BV[DOI]
- Myung-Hwa Jung, "Innate immune responses against rock bream iridovirus (RBIV) infection in rock bream ( Oplegnathus fasciatus ) following poly (I:C) administration", Fish & Shellfish Immunology, 71, pp.171~176 (2017), Elsevier BV[DOI]
- Myung-Hwa Jung, "DNA vaccine encoding myristoylated membrane protein (MMP) of rock bream iridovirus (RBIV) induces protective immunity in rock bream (Oplegnathus fasciatus)", Vaccine, 36(6), pp.802~810 (2018), Elsevier BV[DOI]
- Kosuke Zenke, "Molecular cloning and expression analysis of double-stranded RNA-dependent protein kinase (PKR) in rock bream (Oplegnathus fasciatus)", Veterinary Immunology and Immunopathology, 133(2-4), pp.290~295 (2010), Elsevier BV[DOI]
- Qiang Wan, "Molecular cloning and functional characterization of two duplicated two-cysteine containing type I interferon genes in rock bream Oplegnathus fasciatus", Fish & Shellfish Immunology, 33(4), pp.886~898 (2012), Elsevier BV[DOI]
- S.D.N.K. Bathige, "Interferon regulatory factors 4 and 8 in rock bream, Oplegnathus fasciatus: Structural and expressional evidence for their antimicrobial role in teleosts", Fish & Shellfish Immunology, 33(4), pp.857~871 (2012), Elsevier BV[DOI]
- W.D. Niroshana Wickramaarachchi, "Genomic characterization of interferon regulatory factor 5 from rock bream (Oplegnathus fasciatus) and its role in antiviral defense", Fish & Shellfish Immunology, 37(2), pp.256~267 (2014), Elsevier BV[DOI]
- J W Jin, "Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection", Journal of Fish Diseases, 40(4), pp.583~595 (2016), Wiley[DOI]
- S.D.N.K. Bathige, "Molecular insights of two STAT1 variants from rock bream (Oplegnathus fasciatus) and their transcriptional regulation in response to pathogenic stress, interleukin-10, and tissue injury", Fish & Shellfish Immunology, 69, pp.128~141 (2017), Elsevier BV[DOI]
- Kenneth J. Livak, "Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method", Methods, 25(4), pp.402~408 (2001), Elsevier BV[DOI]
- Wenjing Ruan, "Actin, a reliable marker of internal control?", Clinica Chimica Acta, 385(1-2), pp.1~5 (2007), Elsevier BV[DOI]
- 정명화, "Correlation of virus replication and spleen index in rock bream iridovirus infected rock bream Oplegnathus fasciatus", 한국어병학회지, 32(1), pp.1~8 (2019), 한국어병학회[DOI]
- HJ Kim, "Protection of rainbow trout from infectious hematopoietic necrosis (IHN) by injection of infectious pancreatic necrosis virus (IPNV) or Poly(I:C)", Diseases of Aquatic Organisms, 83, pp.105~113 (2009), Inter-Research Science Center[DOI]
- M‐J Oh, "Field tests of Poly(I:C) immunization with nervous necrosis virus (NNV) in sevenband grouper, <i>Epinephelus septemfasciatus<", i> (Thunberg), 2012, pp.35(3), 187~191[DOI]
- Satheesha Avunje, "Poly (I:C) and imiquimod induced immune responses and their effects on the survival of olive flounder ( Paralichthys olivaceus ) from viral haemorrhagic septicaemia", Fish & Shellfish Immunology, 71, pp.338~345 (2017), Elsevier BV[DOI]
- Yue Jai Kang, "Effect of CpG-ODNs belonging to different classes on resistance of olive flounder (Paralichthys olivaceus) against viral hemorrhagic septicemia virus (VHSV) and Miamiensis avidus (Ciliata; Scuticociliatia) infections", Aquaculture, 324-325, pp.39~43 (2012), Elsevier BV[DOI]
- Xiaohong Huang, "Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides", Fish & Shellfish Immunology, 34(5), pp.1094~1102 (2013), Elsevier BV[DOI]
- G D Trobridge, "Cloning of the rainbow trout (Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein expression in salmon cells", Journal of Virology, 71(7), pp.5304~5311 (1997), American Society for Microbiology[DOI]
- Pierre Boudinot, "Combined DNA Immunization with the Glycoprotein Gene of Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus Induces Double-Specific Protective Immunity and Nonspecific Response in Rainbow Trout", Virology, 249(2), pp.297~306 (1998), Elsevier BV[DOI]
- P.E McLauchlan, "DNA vaccination against viral haemorrhagic septicaemia (VHS) in rainbow trout: size, dose, route of injection and duration of protection—early protection correlates with Mx expression", Fish & Shellfish Immunology, 15(1), pp.39~50 (2003), Elsevier BV[DOI]
- Stephanie J. DeWitte-Orr, "Induction of antiviral genes, Mx and vig-1, by dsRNA and Chum salmon reovirus in rainbow trout monocyte", macrophage and fibroblast cell lines, 2007, pp.23(3), 670~682[DOI]
- Myung-Hwa Jung, "The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock Bream (Oplegnathus fasciatus) Red Blood Cells", Frontiers in Immunology, 10, pp.160~ (2019), Frontiers Media SA[DOI]
- Boudinot P, "vig-1, a new fish gene induced by the rhab- dovirus glycoprotein, has a virus-induced homologue in humans and shares conserved motifs with the MoaA family", Journal of virology, 73(3), pp.1846~1852 (1999)[DOI]
- Eloi R. Verrier, "Early antiviral response and virus-induced genes in fish", Developmental & Comparative Immunology, 35(12), pp.1204~1214 (2011), Elsevier BV[DOI]
- Satheesha Avunje, "Temperature-dependent viral replication and antiviral apoptotic response in viral haemorrhagic septicaemia virus (VHSV)-infected olive flounder (Paralichthys olivaceus)", Fish & Shellfish Immunology, 32(6), pp.1162~1170 (2012), Elsevier BV[DOI]