전염성근괴사증바이러스(IMNV)를 인위감염 시킨 흰다리새우(Litopenaeus vannamei)의 근육에서 나타난 미세 손상과 염증반응에 대한 조직병리학적 특성 연구
Histopathological features of pacific whiteleg shrimp, Litopenaeus vannamei, infected with Infectious Myonecrosis Virus (IMNV) with an emphasis on micro-traumas and inflammatory responses in muscle tissues
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We injected infectious myonecrosis virus (IMNV) to pacific whiteleg shrimp, Litopenaeus vannamei, and observed closely with using light microscope and transmission electron microscope (TEM) for 4-8 days post infection (dpi). As clinical signs, abdominal bodies had mild opaque muscles at 5 dpi. And the mortality was shown at 6 dpi. At 8 dpi, most injected shrimps had severe opaque muscles and humped back that cause of movement disorder. As results of histopathological examinations, local parts of abdominal body muscle had muscle fiber hyalinization, muscle fiber atrophy, rounded muscle fibers, myofibrillar hypertrophy in size, a decrease in number of myofibrils and phagocytosis from the sarcolemmas by multiple hemocytes at 4 dpi. Especially, myofibrillar hypertrophy appeared at the whole or random part of single muscle fiber not in specific locations like the center or edge of muscle fiber. At 6-7 dpi, multiple muscle necrosis, muscle fiber segmentation, myofibril lysis appeared and a few hemocytes were infiltrated at lesions. At 8 dpi, extensive muscle necrosis, multiple myofibril lysis and muscle fiber atrophy were shown, and very few hemocytes were infiltrated. In early stage of infection, local viral myositis with zenker’s degeneration were shown. These lesions appeared multiply after the early stage. In late stage of infection, extensive coagulative muscle necrosis appeared with few of inflammatory response such as hemocytes infiltration. The lack of hemocytes infiltration response at the late stage might be disadvantage for Litopenaeus vannamei to defense against IMNV and to recover, because hematocytes (granulocyte, semi-granulocyte) eliminate pathogen and damaged tissues from infection sites and help recover. As results of the TEM observation, IMNVs that had nonenveloped icosahedral capsid which was 30-40 nm diameter were in myofibril and beside tubules of sarcoplasmic reticulum and moved to the certain direction. The micro-tears and micro-traumas in myofibrils caused muscle fiber necrosis. And semi-granulocytes engulfed IMNVs to eliminate virus.
Abstract
We injected infectious myonecrosis virus (IMNV) to pacific whiteleg shrimp, Litopenaeus vannamei, and observed closely with using light microscope and transmission electron microscope (TEM) for 4-8 days post infection (dpi). As clinical signs, abdominal bodies had mild opaque muscles at 5 dpi. And the mortality was shown at 6 dpi. At 8 dpi, most injected shrimps had severe opaque muscles and humped back that cause of movement disorder. As results of histopathological examinations, local parts of abdominal body muscle had muscle fiber hyalinization, muscle fiber atrophy, rounded muscle fibers, myofibrillar hypertrophy in size, a decrease in number of myofibrils and phagocytosis from the sarcolemmas by multiple hemocytes at 4 dpi. Especially, myofibrillar hypertrophy appeared at the whole or random part of single muscle fiber not in specific locations like the center or edge of muscle fiber. At 6-7 dpi, multiple muscle necrosis, muscle fiber segmentation, myofibril lysis appeared and a few hemocytes were infiltrated at lesions. At 8 dpi, extensive muscle necrosis, multiple myofibril lysis and muscle fiber atrophy were shown, and very few hemocytes were infiltrated. In early stage of infection, local viral myositis with zenker’s degeneration were shown. These lesions appeared multiply after the early stage. In late stage of infection, extensive coagulative muscle necrosis appeared with few of inflammatory response such as hemocytes infiltration. The lack of hemocytes infiltration response at the late stage might be disadvantage for Litopenaeus vannamei to defense against IMNV and to recover, because hematocytes (granulocyte, semi-granulocyte) eliminate pathogen and damaged tissues from infection sites and help recover. As results of the TEM observation, IMNVs that had nonenveloped icosahedral capsid which was 30-40 nm diameter were in myofibril and beside tubules of sarcoplasmic reticulum and moved to the certain direction. The micro-tears and micro-traumas in myofibrils caused muscle fiber necrosis. And semi-granulocytes engulfed IMNVs to eliminate virus.
참고문헌 (32)
- Aguirre-Guzman, G., "Penaeid shrimp immune system", The Thai Journal of Veterinary Medicine, 39(3), pp.205~215 (2009)
- Arulmoorthy, M. P., "Major viral diseases in culturable penaeid shrimps: a review", Aquaculture International, 28(5), pp.1939~1967 (2020)
- Andrade T. P. D., "Real-time reverse transcription polymerase chain reaction assay using TaqMan probe for detection and quantification of infectious myonecrosis virus (IMNV)", Aquaculture, 264, pp.9~15 (2007)
- Costa, A. M., "Immune assessment of farm-reared Penaeus vannamei shrimp naturally infected by IMNV in NE Brazil", Aquaculture, 291(3-4), pp.141~146 (2009)
- Crum-Cianflone, N. F., "Bacterial, fungal, parasitic, and viral myositis", Clinical microbiology reviews, 21(3), pp.473~494 (2008)
- Hameed, A. S., "Studies on the occurrence of Macrobrachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of M. rose- nbergii in India by RT-PCR detection", Aquaculture, 238(1-4), pp.127~133 (2004)
- KOSIS, "Summary table of fishery production trend" (2022), Statistics Korea
- Kunanopparat, A., "Detection of infectious myonecrosis virus using monoclonal antibody specific to N and C fragments of the capsid protein expressed heterologously", Journal of virological methods, 171(1), pp.141~148 (2011)
- 권문경, "우리나라 양식 흰다리새우(Litopenaeus vannamei)에서 발생한 전염성근괴사증(Infectious Myonecrosis) 역학 조사", 수산해양교육연구, 31(1), pp.94~99 (2019), 한국수산해양교육학회[DOI]
- Lee, D., "Viral Shrimp Diseases Listed by the OIE: A Review", Viruses, 14(3), pp.585~ (2022)
- Lightner, D. V., "Diseases of cultured penaeid shrimp", CRC handbook of mariculture, 1, pp.393~486 (1993)
- Lightner, D. V., "Diseases of cultured penaeid shrimp and prawns", Developments in Aquaculture and Fisheries Science(Netherlands), 17, pp.8~10 (1998)
- Lightner, D. V., "Infectious myonecrosis (IMN): a new virus dis- ease of Litopenaeus vannamei", Aquaculture, 242, pp.353~ (2004)
- Lo C. F., "Detection and tis- sue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs", Dis. Aquat. Org., 30, pp.53~72 (1997)
- Martin, G. G., "Fine structure and classification of shrimp hemocytes", Journal of morphol- ogy, 185(3), pp.339~348 (1985)
- Nunan L. M., "Reverse tran- scription polymerase chain reaction (RT-PCR) used for the detection of Taura Syndrome Virus (TSV)in experimentally infected shrimp", Dis. Aquat. Org., 34, pp.87~91 (1998)
- Pestronk, A., "Membrane myopathy: morphological similarities to Duchenne muscular dystrophy", Muscle & Nerve: Official Journal of the American Association of Electrodiagnostic Medicine, 5(3), pp.209~214 (1982)
- Poulos B. T., "Detection of infectious my- onecrosis virus (IMNV) of penaeid shrimp by re- verse-transcriptase polymerase chain reaction (RTPCR)", Dis. Aquat. Org, 73, pp.69~72 (2006)
- Poulos, B., "Purification and characterization of infectious myonecrosis virus of penaeid shrimp", JOURNAL-OF-GENERAL-VIROLOGY, 87, pp.987~996 (2006)
- Prasad, K. P., "Infectious Myonecrosis Virus (IMNV) －An alarming viral pathogen to Penaeid shrimps", Aquaculture, 477, pp.99~105 (2017)
- Prochaska, J., "Evaluation of a commercial SPF Litopenaeus vannamei shrimp breeding program: Resistance to infectious myonecrosis virus (IMNV), Taura syndrome virus (TSV), and white spot syndrome virus (WSSV) from laboratory challenges", Aquaculture, 554, pp.738145~ (2022)
- Sahul Hameed A. S., "Studies on the occurrence of Macro- brachium rosenbergii nodavirus and extra small virus-like particles associated with white tail disease of M. rosenbergii in India by RT-PCR detection", Aquaculture, 238, pp.127~133 (2004)
- Senapin, S., "False rumours of disease outbreaks caused by infectious myonecrosis virus (IMNV) in the whiteleg shrimp in Asia", Journal of negative results in bio- medicine, 10(1), pp.1~5 (2011)
- Sri Widada J., "Ge- nome-based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant fresh water prawn, Macrobrachium rosenbergii:dot-blot, in situ hybridization and RT-PCR", J. Fish Dis., 26, pp.583~590 (2003)
- Srisala, J., "Infectious myonecrosis virus (IMNV) and Decapod iridescent virus 1 (DIV1)detected in captured, wild Penaeus monodon", Aqua- culture, 545, pp.737262~ (2021)
- Tang K. F. J., "Postlarvae and juve- niles of a selected line of Penaeus stylirostris are resistant to infectious hypodermal and hematopoietic necrosis virus infection", Aquaculture, 190, pp.203~210 (2000)
- Tang, J., "Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber com- plexes at the fivefold axes", Proceedings of the Natio- nal Academy of Sciences, 105(45), pp.17526~17531 (2008)
- Tang, K. F., "In situ hybridization demon- strates that Litopenaeus vannamei, L. stylirostris and Penaeus monodon are susceptible to experimental infection with infectious myonecrosis virus (IMNV)", Diseases of aquatic organisms, 63(2-3), pp.261~265 (2005)
- Tang, K. F., "Ultrastructural and se- quence characterization of Penaeus vannamei noda- virus (PvNV) from Belize", Diseases of aquatic or- ganisms, 94(3), pp.179~187 (2011)
- Taukhid, T., "Infectious Myonecrosis Virus (IMNV) in Pacific White Shrimp (Litopenaeus van- namei) in Indonesia", The Israeli Journal of Aquaculture - Bamidgeh, 61(3), pp.255~262 (2009)
- Tung, C. W., "Histological and electron microscopic study on Macro- brachium muscle virus (MMV) infection in the giant freshwater prawn, Macrobrachium rosenbergii (. de Man), cultured in Taiwan", Journal of Fish Diseases, 22(4), pp.319~323 (1999)
- Zierath, J. R., "influence on contractile and metabolic properties", PLoS biology, 2(10), pp.e348~ (2004)