전기화학적인 방법을 이용한 두 개의 오스뮴 고분자 막의 고정화 및 다중 검출 바이오센서에 관한 연구
The Electrochemical Studies of Two Osmium Redox Polymer Films and Their Application for Multi-Detecting Biosensor
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다중 생체시료 검출을 위한 바이오센서 연구를 위하여 각기 두 전위를 갖는 오스뮴 고분자를 함 께 탄소 전극 (Screen Printed Carbon Electrodes) 위에 고정하였다. 새로운 개념의 다중 생체 시료 검출 바이오센서 연구위하여 과산화수소의 환원과 글루코스의 산화에 관여하는 환원 효소와 산화 효소를 각각 이용하였다. 실험 목적에 위하여 염소 작용기 (EO' + 0.520 vs. Ag/AgCl)와 메톡시 작용기 (EO' + 0.150 vs. Ag/AgCl)를 각각 포함하는 두 개의 오스뮴 고분자를 합성하였 다. 전자는 과산화수소의 환원에 대하여 좋은 촉매전류신호를 보였고, 후자는 당의 산화에 대하 여 효과적인 촉매전류신호를 보였다
Abstract
Screen printed carbon electrodes (SPEs) modified with co-immobilized osmium-based redox polymers can be used to apply multi-detecting biosensors. In this study, we report our initial studies of multi-detecting biosensor concepts using two osmium-based redox polymers for horseradish peroxidase-mediated reduction of H2O2 coupled to glucose oxidase-mediated oxidation of glucose. We target to synthesize two osmium redox polymers of potentials use, a chloride-containing redox polymer (EO' + 0.520 vs. Ag/AgCl) and a methoxy-containing redox polymer (EO' + 0.150 vs. Ag/AgCl). The former show good catalytic electrical signals with horseradish peroxidase and the latter's redox polymer is to be an effective redox mediator of glucose oxidation by glucose oxidase
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