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세개의 오스뮴 고분자를 이용한 요산의 전기화학적 측정방법

Electrochemical Detection of Uric Acid using Three Osmium Hydrogels

최영봉|전원용
한국전기화학회 (The Korean Electrochemical Society)|2016.06|전기화학회지 (Journal of the Korean Electrochemical Society)|제19권 제2호|pp. 29-38

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키워드

Uric acidElectrochemical biosensorPoly-osmium hydrogel complexUricaseMediated electron transfer (MET)
주제분류화학

초록

전기화학적 방법을 통한 요산 (Uric acid) 정량분석을 위해 수용성 고분자 (hydrogel polymer) 를 배위시킨 오스뮴 고분자 화합물과 요산 산화효소 (Uricase), 가교를 위한 PEGDGE (poly(ethylene glycol) diglycidyl ether)가 혼합된 용액을 스크린 프린팅된 탄소 전극 (SPCEs) 위에 흡착하여 측정하였다. 수용성 오스뮴 고분자의 전위를 조절하기 위해 리간드인 피리딘링의4번 위치에 다른 전기음성도의 작용기를 갖는 오스뮴 고분자 화합물을 합성하였다. 합성된 오스뮴 고분자 화합물은 PAA-PVI (Poly(acrylic acid)-poly(vinyl imidazole)-[osmium(4,4’- dichloro-2,2’-bipyridine)2Cl]+/2+), PAA-PVI-[osmium(4,4’-dimethyl-2,2’-bipyridine)2Cl]+/2+, PAA-PVI-[osmium(4,4’-dimethoxy-2,2’-bipyridine)2Cl]+/2+이다. 제작된 효소전극은 순환전압전류법 (cyclic voltammetry)을 통해 uric acid에 의한 오스뮴 고분자 화합물들의 산화 촉매 전류(oxidation catalytic current)를 측정하여 uric acid의 농도를 정량적으로 분석할 수 있었다. 오스뮴 고분자 화합물들 중 0.215 V의 산화환원 전위를 갖는 PAA-PVI-[Os(dme-bpy)2Cl]+/2+ (PAA-PVI-osmium(4,4’-dimethyl-2,2’-bipyridine)2Cl]+/2+) 화합물을 이용하여 대표적인 간섭물질인 아스코르브산 (AA)과 포도당 (glucose)의 산화 신호의 간섭효과를 피할 수 있었다. 이를 이용하여 제작된 전극은 0.33 V 전위에서 다양한 농도의 uric acid (1.0, 1.5, 2.0, and 5.0 mM)의 전류를 측정한 결과 r2= 0.9986의 좋은 선형성을 갖는 것을 확인하였다. 이는 복잡하지않은 간단한 방법과 일회용의 전극을 사용하기 때문에 현장현시 검사 (point of care; POC)에적합한 요산측정용 바이오센서로서의 가능성을 확인 할 수 있었다.

Abstract

Screen printed carbon electrodes (SPCEs) with immobilized osmium-based hydrogel redox polymer, uricase and PEGDGE can be used to apply uric acid electrochemical detecting. The osmium redox complexes were synthesized by the coordinating pyridine group having different functional group at 4-position with osmium compounds. The synthesized poly-osmium hydrogel complexes are described as PAA-PVI-[Os(dCl-bpy)2Cl]+/2+, PAA-PVI-[Os(dmebpy) 2Cl]+/2+, PAA-PVI-[Os(dmo-bpy)2Cl]+/2+. The different concentrations of uric acid were measured by cyclic voltammetry technique using enzyme-immobilized SPCEs. The prepared SPCEs using PAA-PVI-[Os(dme-bpy)2Cl]+/2+ showed no interference from common physiologic interferents such as ascorbic acid (AA) or glucose. The resulting electrical currents at 0.33 V vs. Ag/AgCl displayed a good linear response with uric acid concentrations from 1.0 to 5.0 mM. Therefore, this approach allowed the development of a simple, point of care in the medical field, disposable electrochemical uric acid biosensor.

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