노화 생쥐의 복부지방에서 수영운동에 의한 AMPK 와 GLUT4 단백질 변화
Changes of AMPK & GLUT-4 Protein Expression by Swim Exercise in Fat Tissue of Ageing Mice
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본 연구의 목적은 노화된 생쥐에 있어서 수영운동에 따른 AMPK와 GLUT4의 발현을 알아보는 것이다. 이에 본 실험에서는 노화된 생쥐에게 수영운동을 시행하였으며, 몸무게 및 복부지방의 무게를 측정하였고, 지방세포의 크기변화를 Oil-Red 염색법으로, 지방조직에서 AMPK와 Glut-4의 단백질을 분석하였다. 연구대상은 2개월 SWV계 숫컷 생쥐 10마리를 청년대조군(n=10), 12개월 노화된 SWV계 숫컷 생쥐 20마리를 노화대조군과 노화운동군(n=10)의 3군으로 분류하였고, 유산소운동은 주당 5회 40분씩 시행하였으며, 주 1회 체중을 측정하였다. 체중의 변화는 노화대조군이 다른 군에 비해 실험 종결 때까지 높은 체중을 유지하였으나, 노화운동군에서는 수영운동 2주후부터 7주까지 지속적으로 체중이 줄어(p<.05, 001)들었다. 혈당변화는 노화운동군이 다른 두 군에 비해 2주부터 뚜렷한 감소(p<.05)를 보였으며 실험종결 때까지 낮은 혈당을 유지하였다. 8주간 유산소운동 후 복부지방량의 변화는 노화운동군이 다른 두 군에 비해 확실하게 줄어들었고(p<0.001), 노화대조군의 지방세포크기는 비대해져 있었으나, 노화운동군에서는 작은 지방세포의 크기를 보여 주었다. 특히 AMPK와 GLUT-4의 발현은 노화운동군에서 다른 두 군에 비해 뚜렷하게 증가되었다. 이상의 결과를 종합하면 8주간의 수영운동은 노화된 생쥐의 체중 및 복부지방을 감소시켜 지방비대의 억제효과를 나타내었고, AMPK의 단백질발현증가는 지방생성 증가를 억제함을 알 수 있었으며, GLUT-4의 단백질발현증가는 혈당의 수치를 조절하는 효과가 있었다. 따라서 장기간의 수영운동은 노화로 인한 대사의 기능을 향상시켜 대사성질환으로 부터 보호할 수 있을 것으로 생각된다.
Abstract
The present study was designed to examine the protein changes of AMPK and GLUT-4 by swim exercise in fat tissue of aging mice. We performed aerobics exercise by swimming, measured body weight(BW)and measured blood glucose(BG), and performed Oil-Red stain for adipocyte and western blot for AMPK and GLUT-4 protein in fat tissue. SWV(n=30) mice at age of 2 months and 12months old were divided into 3 groups as follows: (1) Old control group (n=10, O-Con), (2) Young control group(n=10, Y-Con) (3) Old+exercise group (n=10, O-Ex). The swimming exercise was performed 5 days /week with duration of 40minutes for 8weeks. BW and BG were measured once per week. Results showed that the BW of O-Con group was is higher than other groups, interestingly, BW of Old-Ex was significantly decreased (p<.05, p<.001) from 2nd. BG of O-Ex groups showed markedly decreased (p<.05, p<.001) from 2 weeks after exercise and continuously lower than other groups. Retroperitoneal fat fed weight of O-Ex group was significantly decreased (p<.001) and adipocyte size of O-Con group was bigger than other groups, but O-Ex group smaller sized adipocyte than other groups. Particulary, AMPK and GLUT-4 which are related with lipid metabolism was markedly inhibited compared to Y-Con group, whereas O-Ex group showed increased of AMPK and GLUT-4 protein expression than other groups. These results suggest that swim exercise for 8 week should inhibit adipocyte hypertrophy by controling the BW and decreasing retroperitoneal fat fed through AMPK protein expression and controling protective of BG the by decreasing the BG through GLUT-4 protein expression in fat tissue of aging mice. Thus, exercise may be helpful to improvement of lipid metabolism function and may be protective effect from lipid metabolism disease.
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