본문 바로가기
KERD
논문KCI 등재

Human collagen alpha-2 type I stimulates collagen synthesis, wound healing, and elastin production in normal human dermal fibroblasts (HDFs)

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류화학

초록

Skin aging appears to be the result of overlapping intrinsic (including genetic and hormonal factors) and extrinsic (external environment including chronic light exposure, chemicals, and toxins) processes. These factors cause decreases in the synthesis of collagen type I and elastin in fibroblasts and increases in the melanin in melanocytes. Collagen Type I is the most abundant type of collagen and is a major structural protein in human body tissues. In previous studies, many products containing collagen derived from land and marine animals as well as other sources have been used for a wide range of purposes in cosmetics and food. However, to our knowledge, the effects of human collagenderived peptides on improvements in skin condition have not been investigated. Here we isolate and identify the domain of a human COL1A2-derived protein which promotes fibroblast cell proliferation and collagen type I synthesis. This human COL 1A2-derived peptide enhances wound healing and elastin production. Finally, the human collagen alpha-2 type I-derived peptide (SMM) ameliorates collagen type I synthesis, cell proliferation, cell migration, and elastin synthesis, supporting a significant anti-wrinkle effect. Collectively, these results demonstrate that human collagen alpha-2 type I-derived peptides is practically accessible in both cosmetics and food, with the goal of improving skin condition.

Abstract

Skin aging appears to be the result of overlapping intrinsic (including genetic and hormonal factors) and extrinsic (external environment including chronic light exposure, chemicals, and toxins) processes. These factors cause decreases in the synthesis of collagen type I and elastin in fibroblasts and increases in the melanin in melanocytes. Collagen Type I is the most abundant type of collagen and is a major structural protein in human body tissues. In previous studies, many products containing collagen derived from land and marine animals as well as other sources have been used for a wide range of purposes in cosmetics and food. However, to our knowledge, the effects of human collagenderived peptides on improvements in skin condition have not been investigated. Here we isolate and identify the domain of a human COL1A2-derived protein which promotes fibroblast cell proliferation and collagen type I synthesis. This human COL 1A2-derived peptide enhances wound healing and elastin production. Finally, the human collagen alpha-2 type I-derived peptide (SMM) ameliorates collagen type I synthesis, cell proliferation, cell migration, and elastin synthesis, supporting a significant anti-wrinkle effect. Collectively, these results demonstrate that human collagen alpha-2 type I-derived peptides is practically accessible in both cosmetics and food, with the goal of improving skin condition.

참고문헌 (41)

  1. El-Domyati M, "Intrinsic aging vs. photoaging : a comparative histopathological, immunohistochemical, and ultrastructural study of skin", Exp Dermatol, 11, pp.398~405 (2002)
  2. Ganceviciene R, "Skin anti-aging strategies", Dermatoendocrinol, 4, pp.308~319 (2012)
  3. Rendon MI, "Review of skin-lightening agents", Dermatol Surg, 31, pp.886~889 (2005)
  4. Petit L, "Skin-lightening products revisited", Int J Cosmet Sci, 25, pp.169~181 (2003)
  5. Kim WS, "Antiwrinkle effect of adipose-derived stem cell : activation of dermal fibroblast by secretory factors", J Dermatol Sci, 53, pp.96~102 (2009)
  6. Kim J, "Dual functional bioactive-peptide, AIMP1-derived peptide(AdP), for anti-aging", J Cosmet Dermatol, 18, pp.251~257 (2019)
  7. Edgar S, "Effects of collagen-derived bioactive peptides and natural antioxidant compounds on proliferation and matrix protein synthesis by cultured normal human dermal fibroblasts", Sci Rep, 8, pp.10474~ (2018)
  8. van Zuijlen PP, "Collagen morphology in human skin and scar tissue : no adaptations in response to mechanical loading at joints", Burns, 29, pp.423~431 (2003)
  9. Gelse K, "Collagens-structure, function, and biosynthesis", Adv Drug Deliv Rev, 55, pp.1531~1546 (2003)
  10. Singer AJ, "Cutaneous wound healing", N Engl J Med, 341, pp.738~746 (1999)
  11. Imanishi J, "Growth factors : importance in wound healing and maintenance of transparency of the cornea", Prog Retin Eye Res, 19, pp.113~129 (2000)
  12. Park JS, "Epidermal growth factor(EGF)antagonizes transforming growth factor(TGF)-beta1-induced collagen lattice contraction by human skin fibroblasts", Biol Pharm Bull, 23, pp.1517~1520 (2000)
  13. Van Cauter E, "Physiology of growth hormone secretion during sleep", J Pediatr, 128, pp.S32~37 (1996)
  14. Bartke A, "Growth hormone and aging : a challenging controversy", Clin Interv Aging, 3, pp.659~665 (2008)
  15. Sonntag WE, "Diverse roles of growth hormone and insulin-like growth factor-1 in mammalian aging : progress and controversies", J Gerontol A Biol Sci Med Sci, 67, pp.587~598 (2012)
  16. Sontakke SB, "Orally Available Collagen Tripeptide : Enzymatic Stability, Intestinal Permeability, and Absorption of Gly-Pro-Hyp and Pro-Hyp", J Agric Food Chem, 64, pp.7127~7133 (2016)
  17. Watanabe-Kamiyama M, "Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats", J Agric Food Chem, 58, pp.835~841 (2010)
  18. Asserin J, "The effect of oral collagen peptide supplementation on skin moisture and the dermal collagen network : evidence from an ex vivo model and randomized, placebo-controlled clinical trials", J Cosmet Dermatol, 14, pp.291~301 (2015)
  19. Inoue N, "Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study", J Sci Food Agric, 96, pp.4077~4081 (2016)
  20. Konig D, "Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women-A Randomized Controlled Study", Nutrients, 10, pp.97~ (2018)
  21. Zdzieblik D, "Collagen peptide supplementation in combination with resistance training improves body composition and increases muscle strength in elderly sarcopenic men : a randomised controlled trial", Br J Nutr, 114, pp.1237~1245 (2015)
  22. Hu Z, "Marine Collagen Peptides from the Skin of Nile Tilapia(Oreochromis niloticus) : Characterization and Wound Healing Evaluation", Mar Drugs, 15, pp.102~ (2017)
  23. Minh Thuy le T, "Isolation and characterization of acid-soluble collagen from the scales of marine fishes from Japan and Vietnam", Food Chem, 149, pp.264~270 (2014)
  24. Felician FF, "Collagen from Marine Biological Sources and Medical Applications", Chem Biodivers, 15, pp.e1700557~ (2018)
  25. Wang B, "Isolation and characterization of collagen and antioxidant collagen peptides from scales of croceine croaker(Pseudosciaena crocea)", Mar Drugs, 11, pp.4641~4661 (2013)
  26. Tanaka M, "Effects of collagen peptide ingestion on UV-B-induced skin damage", Biosci Biotechnol Biochem, 73, pp.930~932 (2009)
  27. 이준규, "Comparison of Collagen Characteristics of Sea- and Freshwater-Rainbow Trout Skin", Food Science and Biotechnology, 25(1), pp.131~136 (2016), 한국식품과학회[DOI]
  28. Trojanowska M, "Pathogenesis of fibrosis : type 1 collagen and the skin", J Mol Med (Berl), 76, pp.266~274 (1998)
  29. Chiquet M, "Regulation of extracellular matrix synthesis by mechanical stress", Biochem Cell Biol, 74, pp.737~744 (1996)
  30. Majora M, "Functional consequences of mitochondrial DNA deletions in human skin fibroblasts : increased contractile strength in collagen lattices is due to oxidative stress-induced lysyl oxidase activity", Am J Pathol, 175, pp.1019~1029 (2009)
  31. Seo WY, "Production of recombinant human procollagen type I C-terminal propeptide and establishment of a sandwich ELISA for quantification", Sci Rep, 7, pp.15946~ (2017)
  32. Chen CZ, "The Scar-in-a-Jar : studying potential antifibrotic compounds from the epigenetic to extracellular level in a single well", Br J Pharmacol, 158, pp.1196~1209 (2009)
  33. Adelmann-Grill BC, "Chemotactic migration of normal dermal fibroblasts towards epidermal growth factor and its modulation by platelet-derived growth factor and transforming growth factor-beta", Eur J Cell Biol, 51, pp.322~326 (1990)
  34. Walter MN, "Mesenchymal stem cell-conditioned medium accelerates skin wound healing : an in vitro study of fibroblast and keratinocyte scratch assays", Exp Cell Res, 316, pp.1271~1281 (2010)
  35. Quan T, "Reduced expression of connective tissue growth factor(CTGF", CCN2)mediates collagen loss in chronologically aged human skin, 2010, pp.130, 415~424
  36. Seite S, "Elastin changes during chronological and photo-ageing : the important role of lysozyme", J Eur Acad Dermatol Venereol, 20, pp.980~987 (2006)
  37. Liu Z, "Collagen peptides promote photoaging skin cell repair by activating the TGFbeta", Smad pathway and depressing collagen degradation, 2019, pp.10, 6121~6134
  38. Fisher GJ, "Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo", J Invest Dermatol, 117, pp.219~226 (2001)
  39. Sugimoto K, "Matrix metalloproteinase promotes elastic fiber degradation in ligamentum flavum degeneration", PLoS One, 13, pp.e0200872~ (2018)
  40. Chung JH, "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo", J Invest Dermatol, 117, pp.1218~1224 (2001)
  41. Martinotti S, "Scratch Wound Healing Assay", Methods Mol Biol, 2109, pp.225~229 (2020)

관련 논문AI추천

Effects of human collagen α-1 type I-derived proteins on collagen synthesis and elastin production in human dermal fibroblasts김경진, Dongryeol Ryu, Min Cheol Shin, Sang Bae Lee, Su Hwan Kim, Su Jin Hwang, Woo-Young Seo, Yelin Jeong, Young-Jin Choi생화학분자생물학회 · BMB Reports2021 · 제54권 제6호Development of a highly effective recombinant protein from human collagen type III Alpha 1 (COL3A1) to enhance human skin cell functionality김경진, 김영준, 이상배, Eun Kyung Jeong, HyunJoon Gi, Seokwon Han, Woo-Young Seo, Young Un Kim생화학분자생물학회 · BMB Reports2024 · 제57권 제9호Multifunctional Skin Dermal Extracellular Matrix Enabling Skin-Relevant Bioactivity for Tissue Remodeling, Hydration, and Anti-HyperpigmentationCho Jung Ho, Cho Seung-Woo, Han Seung Yeop, Kim Yu Heun, Park Sewon한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2026 · 제36권 Skin ECM Provides a Bio-Derived Platform for Supporting Dermal Renewal and Matrix SynthesisChoi Soojeong, Cho Jung Ho, Cho Seung-Woo, Jin Yoonhee, Kim Ha Jin, Lee Mi Jeong, Lee Seulbi, Lee Seungrok, Park Sewon한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Convergence Study on Preparation of Anti-aging Peptides from Fish Collagen Hydrolysates배인영, 이현규, 이현준, 제현정, 한유경한국융합학회 · 한국융합학회논문지2020 · 제11권 제5호Skin Anti-Aging and Moisturizing Effects of Low-Molecular-Weight Collagen Peptide Supplementation in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Clinical TrialAhn Dong Kyu, Kim Han Jo, Kim Jong Hoon, Lee Eunkyoung, Lee Hae Kwang, Lee Sunghee, Shin Jin Hee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권

이 간행물의 다른 논문 (7건)