Species Identification Method via Unique Genetic Markers for Reticulitermes kanmonensis
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Invasive alien species, along with climate change, are major contributing factors to biodiversity loss, and their unintentional introduction through imported goods is increasing. Termites, as wood-feeding insects, pose a significant threat when introduced into Korea via imported wood, necessitating a rapid and accurate identification method. In this study, we developed a diagnostic method based on species-specific genetic sequences for identifying Reticulitermes kanmonensis, a recently identified species. Termite samples were collected from Wanju-gun, Jeollabuk-do, and subjected to whole-genome sequencing to pinpoint species-specific genetic sequences. Utilizing these sequences, we designed primer sets and employed TaqMan-based primer sets and qPCR analysis to select the final primer sets capable of rapidly distinguishing R. kanmonensis. The genetic detection method developed here offers a rapid means of identifying alien termite species, likely enhancing termite management and quarantine practices in Korea.
Abstract
Invasive alien species, along with climate change, are major contributing factors to biodiversity loss, and their unintentional introduction through imported goods is increasing. Termites, as wood-feeding insects, pose a significant threat when introduced into Korea via imported wood, necessitating a rapid and accurate identification method. In this study, we developed a diagnostic method based on species-specific genetic sequences for identifying Reticulitermes kanmonensis, a recently identified species. Termite samples were collected from Wanju-gun, Jeollabuk-do, and subjected to whole-genome sequencing to pinpoint species-specific genetic sequences. Utilizing these sequences, we designed primer sets and employed TaqMan-based primer sets and qPCR analysis to select the final primer sets capable of rapidly distinguishing R. kanmonensis. The genetic detection method developed here offers a rapid means of identifying alien termite species, likely enhancing termite management and quarantine practices in Korea.
참고문헌 (12)
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