국내 서식 흰개미의 개체 및 군체 식별을 위한 Nile Blue A 염색법 적용성 평가
Applicability evaluation of Nile Blue A dye to Korean termite (Reticulitermes speratus kyushuensis)
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참고문헌
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초록
이 연구는 국외에서 지중흰개미의 생태와 방제 연구를 위해 사용하는 Nile Blue A 염색약이 국내 전역에 서식하는 R. s. kyushuensis에 대한 적용 여부 및 적정 조건을 평가하기 위해 수행되었다.
Abstract
Dye markers are used in the study of subterranean termites to determine colony territories and population sizes. Dyes not only allow the study of termite biology, but can also be used for control purposes. Although Reticulitermes speratus kyushuensis causes damage to wooden historical properties in Korea, the dye markers have not been applied. Therefore, we evaluated the applicability of Nile Blue A dye for R. s. kyushuensis. Filter papers dyed with concentrations of 0.05%, 0.10%, and 0.25% (w/w) were each fed to termite populations through a no-choice feeding test for 1, 3, or 7 days. After 4 weeks, the survival rate, dyeing rate, and maintenance of dyeing were recorded. The results showed high survival rates with lower dye concentrations and shorter feeding periods. Among the combinations of concentrations and days, high survival rates, dyeing rates, and dyeing maintenance periods were found at concentrations of 0.05% and 0.10% for 3 days of feeding. These maximized the survivorship and visibility. The results from the current study can be applied to investigate the ecology and control of R. s. kyushuensis in both the laboratory and field.
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