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SRY Gene Sequence Analysis of the Long-tailed Goral (Naemorhedus caudatus)

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키워드

주제분류축산학

초록

The long-tailed goral (Naemorhedus caudatus) is classified as 'vulnerable' by the International Union for Conservation of Nature and has been designated an endangered species requiring conservation and management in Korea. The sex determination region of the Y (SRY) gene is a useful marker for the study of paternal lineages; however, the SRY gene of the goral has not yet been sequenced. In this study, the nucleotide sequence of the SRY gene of long-tailed gorals was determined based on the sequence of the SRY gene of goats (Capra hircus). The obtained sequences were aligned with those of other species in the Bovidae family. The long-tailed goral SRY gene comprised 720 base pairs, and its nucleotide and protein sequences were identical to those of goats, sheep (Ovis aries), and cattle (Bos taurus) by 96%, 97%, and 93%, respectively. The results of phylogenetic insights obtained from this study constitute important references for genetic diversity and pedigrees studies of male long-tailed gorals and closely related species.

Abstract

The long-tailed goral (Naemorhedus caudatus) is classified as 'vulnerable' by the International Union for Conservation of Nature and has been designated an endangered species requiring conservation and management in Korea. The sex determination region of the Y (SRY) gene is a useful marker for the study of paternal lineages; however, the SRY gene of the goral has not yet been sequenced. In this study, the nucleotide sequence of the SRY gene of long-tailed gorals was determined based on the sequence of the SRY gene of goats (Capra hircus). The obtained sequences were aligned with those of other species in the Bovidae family. The long-tailed goral SRY gene comprised 720 base pairs, and its nucleotide and protein sequences were identical to those of goats, sheep (Ovis aries), and cattle (Bos taurus) by 96%, 97%, and 93%, respectively. The results of phylogenetic insights obtained from this study constitute important references for genetic diversity and pedigrees studies of male long-tailed gorals and closely related species.

참고문헌 (13)

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  2. 정철운, "주왕산국립공원에 서식하는 포유동물의 고도에 따른 분포 특징의 분석", Journal of Ecology and Environment, 29(6), pp.511~520 (2006), 한국생태학회
  3. Clawson M. L, "Male-specific SRY and ZFY haplotypes in US beef cattle", Anim. Genet, 35, pp.245~264 (2004)[DOI]
  4. Duckworth, J. W., "The IUCN Red List of Threatened Species 2008", pp.e.T14295A4~ (2008)
  5. Fu, Q., "Cloning the swamp buffalo SRY gene for embryo sexing with multiplex nested PCR", Theriogenology, 68, pp.1211~1218 (2007)[DOI]
  6. Hassanin, A., "Molecular systematics of the subfamily Caprinae(Artiodactyla, Bovidae)as determined from cytochrome b sequences", J. Mamm. Evol, 5(3), pp.217~236 (1998)[DOI]
  7. López-Sepulcre, A., "Reproductive conflict delays the recovery of an endangered social species", J. Anim. Ecol, 78, pp.219~225 (2009)[DOI]
  8. Meadows, J. R. S, "Nucleotide diversity of the ovine Y chromosome", Anim. Genet, 35, pp.37985~ (2004)[DOI]
  9. Payen, E. J., "Comparative HMG-box sequences of the SRY gene between sheep, cattle and goats", Nucleic Acids Res, 21, pp.2772~ (1993)[DOI]
  10. Payen, E. J., "Sequence evolution of SRY gene within Bovidae family", Mamm. Genome, 5, pp.723~725 (1994)
  11. Prashant, K., "Complete nucleotide sequencing, SNP identification and characterization of SRY gene in Indian Sangamneri goat", Afr. J. Biotechnol, 8(13), pp.2939~2942 (2009)
  12. Weber, J. A., "Genomic perspectives on adaptation and conservation in the endangered long-tailed goral (Naemorhedus caudatus)", BioRxiv, 505156, ~ (2018)
  13. Wedekind, C., "Manpulating sex ratios for conservation : short-term risks and long-termbenefits", Anim. Conserv, 5(1), pp.13~20 (2002)[DOI]

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