소맥 위주의 사료 내 Xylanase의 첨가가 육성돈의 사양성적, 영양소 소화율, 혈액성상, 분내 휘발성 지방산 및 암모니아성 질소 농도에 미치는 영향
Effects of Xylanase Supplementation on Growth Performance, Nutrient Digestibility, Blood Metabolites, Fecal Volatile Fatty Acids and Ammonia Nitrogen in Growing Pigs Fed Wheat-Based Diets
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본 연구는 소맥 위주 사료에 xylanase 효소제의 첨가가 육성돈의 사양성적, 영양소 소화율, 혈액성상, 분 중 휘발성 지방산 및 암모니아성 질소 농도에 미치는 영향을 알아보기 위하여 실시하였다. 총 192두(4처리, 8반복, 반복당 6두)의 육성돈(25.14±0.11 kg)을 공시하여 xylanase 첨가수준(0, 0.0125, 0.025, 0.0375%)으로 6주간 사양시험을 실시하였다. xylanase의 첨가수준이 증가함에 따라 전체 사양구간에서의 일당증체량(ADG), 일일사료섭취량(ADFI) 및 사료요구율(FCR)이 유의적으로 개선되는 효과를 나타냈다(p<005). 영양소 소화율에 있어서, xylanase 첨가수준이 증가함에 따라 phase Ⅰ에서는 건물 및 에너지, phase Ⅱ에서는 조단백질 소화율이 유의적으로 개선되었으며, 또한 육성돈의 혈중 GLU 농도는 사료 내 xylanase의 첨가수준이 증가함에 따라 유의적으로 증가하는 효과를 보였다(p<0.05). 반면에, 휘발성 지방산 및 암모니아성 질소 농도에서 xylanase의 유의적인 첨가효과는 나타나지 않았다(p>0.05). 결론적으로, 소맥 위주의 사료 내 xylanase의 첨가는 육성돈의 사양성적, 영양소 소화율 및 혈중 GLU 농도를 증가시키는데 긍정적인 효과를 보였으며, 육성돈 사료내 소맥을 주원료로 사용할 경우 xylanse의 적정 첨가수준은 0.0375%으로 사료된다.
Abstract
This study was conducted to investigate the effects of dietary xylanase supplements at various levels on growth performance, nutrient digestibility, blood metabolites, fecal volatile fatty acids, and ammonia nitrogen in growing pigs fed wheat-based diets. One hundred and ninety-two growing pigs (Landrace×Yorkshire×Duroc) with an average body weight of 25.14±0.11 kg were randomly assigned to four treatments on the basis of their initial body weight. Each treatment had eight replicates with six pigs. The experimental period was divided into phases Ⅰ and Ⅱ(each 21 days). The dietary treatments were: 1) Basal diet (T1, control), 2) T2 (control+0.0125% xylanase), 3) T3 (control+0.025% xylanase), 4) T4 (control+0.0375% xylanase). Improved average daily gain, average feed intake, and feed conversion ratio were observed with increasing xylanase supplemental levels throughout overall experimental periods (p<0.05). In the nutrient digestibility, the pigs fed diets supplemented with xylanase showed a linear increase in dry matter, energy in phases Ⅰ and Ⅱ, and crude protein in phase Ⅱ with the progressive supplemental levels of dietary xylanase (p<0.05). Blood glucose concentration showed a significantly increased effect in linear impact with increasing xylanase supplementation in diets. Dietary xylanase treatments did not affect fecal volatile fatty acids and ammonia nitrogen concentration. The results of the present study demonstrate the potential of dietary supplementation of xylanase in improving growth performance, nutrient digestibility, and blood glucose concentration in growing pigs fed wheat-based diets. In addition, this study suggests that the proper supplemental level of xylanase is 0.0375% in growing pigs fed wheat-based diets.
참고문헌 (38)
- Abelilla, J. J., "Degradation of dietary fiber in the stomach, small intestine, and large intestine of growing pigs fed corn-or wheat-based diets without or with microbial xylanase", J. Anim. Sci, 97(1), pp.338~352 (2019)
- AOAC, "Official Methods of Analysis" (2007), Association of Official Analytical Chemists
- Aranda-Aguirre, E., "A systematic-review on the role of exogenous enzymes on the productive performance at weaning, growing and finishing in pigs", Vet. Anim. Sci, 14, pp.100195~ (2021)
- Bedford, M. R., "Exogenous enzymes in monogastric nutrition—their current value and future benefits", Anim. Feed. Sci. Technol, 86(1-2), pp.1~13 (2000)
- Boontiam, W., "Xylanase impact beyond performance : Effects on gut structure, faecal volatile fatty acid content and ammonia emissions in weaned piglets fed diets containing fibrous ingredients", Animals, 12(21), pp.30~43 (2022)
- Bueno, D., "Enzymatic cocktail formulation for xylan hydrolysis into xylose and xylooligosaccharides", Molecules, 28(2), pp.624~635 (2023)
- Buksa, K., "Characterization of water and alkali extractable arabinoxylan from wheat and rye under standardized conditions", J. Food Sci. Technol, 53(3), pp.1389~1398 (2016)
- Cao, L., "A newroute to the considerable enhancement of glucose oxidase(GOx)activity : the simple assembly of a complex from CdTe quantum dots and GOx, and its glucose sensing", Chem. Eur. J, 14(31), pp.9633~9640 (2008)
- Chaney, A. L., "Modified regents for determination of urea and ammonia", Clin. Chem, 8(2), pp.130~132 (1962)
- Corrêa-Oliveira, R., "Regulation of immune cell function by short-chain fatty acids", Clin. Transl. Immunol, 5(4), pp.73~80 (2016)
- Bing Dong, "Effects of xylanase supplementation to wheat-based diets on growth performance, nutrient digestibility and gut microbes in weanling pigs", Animal Bioscience, 31(9), pp.1491~1499 (2018), 아세아·태평양축산학회[DOI]
- Engberg, R. M., "Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract", Poult. Sci, 83(6), pp.925~938 (2004)
- Izydorczyk, S. M., "Functional Food Carbohydrates", pp.250~290 (2007), CRC Press
- Jha, R., "Sustainable Swine Nutrition", pp.285~306 (2022), Wiley & Sons, Ltd
- Jiao, A., "Short chain fatty acids could prevent fat deposition in pigs via regulating related hormones and genes", Food Funct, 11(2), pp.1845~1855 (2020)
- Kerr, B. J., "Soluble corn fiber, resistant corn starch, and protected butyrate effects on performance, gastrointestinal volatile fatty acids, and apparent total tract digestibility of calcium and phosphorus in nursery pigs", J. Anim. Sci, 101, pp.1~9 (2023)
- Khadem, A., "Does release of encapsulated nutrients have an important role in the efficacy of xylanase in broilers?", Poult. Sci, 95(5), pp.1066~1076 (2016)
- Lærke, H. N., "Effect of xylanases on ileal viscosity, intestinal fiber modification, and apparent ileal fiber and nutrient digestibility of rye and wheat in growing pigs", J. Anim. Sci, 93(9), pp.4323~4335 (2015)
- Lan, R., "Effects of xylanase supplementation on growth performance, nutrient digestibility, blood parameters, fecal microbiota, fecal score and fecal noxious gas emission of weaning pigs fed corn-soybean meal-based diet", Anim. Sci. J, 88(9), pp.1398~1405 (2017)
- Li, Y., "Identification and characterization of a novel endo-β-1, 4-xylanase from streptomyces sp. T7 and its application in xylo-oligosaccharide production", Molecules, 27(8), pp.2516~ (2022)
- Nortey, T. N., "Effects of xylanase supplementation on the apparent digestibility and digestible content of energy, amino acids, phosphorus, and calcium in wheat and wheat by-products from dry milling fed to grower pigs", J. Anim. Sci, 86(12), pp.3450~3464 (2008)
- NRC(National Research Council), "Nutrient requirements of swine" (2012), National Academy Press
- O'Connell, J. M., "The effect of cereal type and exogenous enzyme supplementation in pig diets on nutrient digestibility, intestinal microflora, volatile fatty acid concentration and manure ammonia emissions from finisher pigs", Anim. Sci, 81(3), pp.357~364 (2005)
- O'Shea, C. J., "The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower–finisher pigs", Anim. Feed Sci. Technol, 189, pp.88~97 (2014)
- Owusu-Asiedu, A., "Effect of xylanase and β-glucanase on growth performance and nutrient digestibility in piglets fed wheat– barley-based diets", Livest. Sci, 134(1-3), pp.76~78 (2010)
- Oyeagu, C. E., "Effect of dietary supplementation of Aspergillus xylanase on broiler chickens performance", Iran. J. Appl. Anim. Sci, 9(4), pp.693~708 (2019)
- Petry, A. L., "Xylanase increased the energetic contribution of fiber and improved the oxidative status, gut barrier integrity, and growth performance of growing pigs fed insoluble corn-based fiber", J. Anim. Sci, 98(7), pp.1~11 (2020)
- Rauw, W. M., "Behaviour influences cholesterol plasma levels in a pig model", Animal, 1(6), pp.865~871 (2007)
- Ribeiro, T., "Xylo-oligosaccharides display a prebiotic activity when used to supplement wheat or corn-based diets for broilers", Poult. Sci, 97(12), pp.4330~4341 (2018)
- Rosenfelder, P., "Nutritive value of wheat and wheat by-products in pig nutrition : A review", Anim. Feed Sci. Technol, 185(3-4), pp.107~125 (2013)
- SAS, "Users Guide:Statistics, Version 8.2" (2012), SAS Institute
- Saville, B. A., "Prebiotics and Probiotics-Potential Benefits in Nutrition and Health" (2019), IntechOpen
- Taylor, A. E., "The effects of xylanase on grower pig performance, concentrations of volatile fatty acids and peptide YY in portal and peripheral blood", Animal, 12(12), pp.2499~2504 (2018)
- Van Hees, H. M. J., "Dietary fibre enrichment of supplemental feed modulates the development of the intestinal tract in suckling piglets", J. Anim. Sci. Biotechnol, 10, pp.83~93 (2019)
- Van Hoeck, V., "Xylanase impact beyond performance : Aprebiotic approach in broiler chickens", J. Appl. Poult. Res, 30(4), pp.100193~ (2021)
- Wolin, M. J., "Human Intestinal Microflora in Health and Disease", pp.147~165 (1993), Academic Press
- Zhao, J. B., "Effect of wheat bran on apparent total tract digestibility, growth performance, fecal microbiota and their metabolites in growing pigs", Anim. Feed Sci. Technol, 239, pp.14~26 (2018)
- Zhao, J., "Fiber-rich foods affected gut bacterial community and short-chain fatty acids production in pig model", J. Funct. Foods, 57, pp.266~274 (2019)
