편백정유의 집먼지 진드기에 의해 활성화된 기관지 상피세포에 미치는 영향
Effect of Essential Oil from Chamacecyparis Obtusa on the Activation of Bronchial Epithelial Cells by House Dust Mite
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background and Objectives：The essential oil (EO) from Chamacecyparis obtusa (C. obtusa) has immunological activities with anti-oxidant, anti-inflammatory, and anti-bacterial characteristics. The aim of this study was to investigate the effect of EO from C. obtusa on the house dust mite induced chemical mediator production from bronchial epithelial cells. Materials and Methods：BEAS-2B cells were exposed to Dermatophagoides pteronyssinus (DP) and Dermatophagoides farina (DF) with or without various concentration of EO from C. obtusa. Interleukin-6 (IL-6) and IL-8 were measured to determine the activation of bronchial epithelial cells. Nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinase (MAPK) expression and activity were determined with western blot analysis. Results：DP and DF induced IL-6 and IL-8 production from BEAS-2B cells and these chemical mediator productions were effectively inhibited by 0.02-0.04% of EO. DP and DF enhanced NF-κB and AP-1 transcription factor expression and EO from C. obtusa effectively suppressed the expression and activity of theses transcription factors. Conclusions：EO from C. obtusa suppressed the production of IL-6 and IL-8 with inhibition of the expression and activity of NF-κB and AP-1. EO from C. obtusa is a potential value for the treatment of allergic airway inflammatory diseases
Abstract
Background and Objectives：The essential oil (EO) from Chamacecyparis obtusa (C. obtusa) has immunological activities with anti-oxidant, anti-inflammatory, and anti-bacterial characteristics. The aim of this study was to investigate the effect of EO from C. obtusa on the house dust mite induced chemical mediator production from bronchial epithelial cells. Materials and Methods：BEAS-2B cells were exposed to Dermatophagoides pteronyssinus (DP) and Dermatophagoides farina (DF) with or without various concentration of EO from C. obtusa. Interleukin-6 (IL-6) and IL-8 were measured to determine the activation of bronchial epithelial cells. Nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinase (MAPK) expression and activity were determined with western blot analysis. Results：DP and DF induced IL-6 and IL-8 production from BEAS-2B cells and these chemical mediator productions were effectively inhibited by 0.02-0.04% of EO. DP and DF enhanced NF-κB and AP-1 transcription factor expression and EO from C. obtusa effectively suppressed the expression and activity of theses transcription factors. Conclusions：EO from C. obtusa suppressed the production of IL-6 and IL-8 with inhibition of the expression and activity of NF-κB and AP-1. EO from C. obtusa is a potential value for the treatment of allergic airway inflammatory diseases
참고문헌 (21)
- Synek M, "Cellular infiltration of the airways in asthma of varying severity", Am J Respir Crit Care Med, 154(1), pp.224~230 (1996)
- Tomee JF, "Interaction between inhalant extracts and airway epithelial cells: effect on cytokine production and cell detachment", J Allergy Clin Immunol, 102(1), pp.78~85 (1998)
- Adam E, "The house dust mite allergen Der p 1, unlike Der p3, stimulates the expression of interleukin-8 in human airway epithelial cells via a proteinase-activated receptor-2-independent mechanism", J Biol Chem, 281(11), pp.6910~6923 (2006)
- Steelant B, "Impaired barrier function in patients with house dust mite–induced allergic rhinitis is accompanied by decreased occludin and zonula occludens-1expression", J Allergy Clin Immunol, 137(4), pp.1043~1053 (2016)
- Kato A, "Beyond inflammation: airway epithelial cells are at the interface of innate and adaptive immunity", Curr Opin Immunol, 19(6), pp.711~720 (2007)
- Weitnauer M, "Control of local immunity by airway epithelial cells", Mucosal Immunol, 9(2), pp.287~298 (2016)
- Whitsett JA, "Respiratory epithelial cells orchestrate pulmonary innate immunity", Nat Immunol, 16(1), pp.27~35 (2015)
- 강수경, "Phytoncide Extracted from Pinecone Decreases LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells", Journal of Microbiology and Biotechnology, 26(3), pp.579~587 (2016), 한국미생물·생명공학회[DOI]
- Lee HJ, "In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extract", J Ethonopharmacol, 103(2), pp.208~216 (2006)
- Li Q, "Effect of phytoncide from trees on human natural killer cell function", Int J Immunopathol Pharmacol, 22(4), pp.951~959 (2009)
- Tu PT, "Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of Alpinia zerumbet", Molecules, 20(9), pp.16723~16740 (2015)
- Pan HH, "Epithelial growth factor receptor tyrosine kinase inhibitors alleviate house dust mite allergen Der p2-induced IL-6 and IL-8", Environ Toxicol, 34(4), pp.476~485 (2019)
- Park HS, "The roles of phytochemicals in bronchial asthma", Molecules, 15(10), pp.6810~6834 (2010)
- Lee HO, "Antimicrobial effects of Chamacecyparis obtuse essential oil", Kor J Appl Microbial Biotechnol, 29(4), pp.253~257 (2001)
- 신승헌, "말초혈액 단핵구 활성화에 대한 편백정유의 항염증 작용", 대한이비인후-두경부외과학회지, 60(11), pp.565~569 (2017), 대한이비인후과학회[DOI]
- An BS, "Anti-inflammatory effects of essential oils from Chamaecyparis obtusa via the cyclooxygenase-2 pathway in rats", Mol Med Rep, 8(1), pp.255~259 (2013)
- Park Y, "Anti-inflammatory effects of essential oils extracted from Chamaecyparis obtusa on murine models of inflammation and RAW 264.7 cells", Mol Med Rep, 13(4), pp.3335~3341 (2016)
- 신승헌, "Th2 Responses Elicited by Nasal Epithelial Cells Exposed to House Dust Mite Extract", Clinical and Experimental Otorhinolaryngology, 2(4), pp.175~180 (2009), 대한이비인후과학회
- Fujioka S, "NF-kappa B and AP-1 connection: mechanism of NF-kappa B-dependent regulation of AP-1 activity", Mol Cell Biol, 24(17), pp.7806~7819 (2004)
- Vroling AB, "Primary nasal epithelium exposed to house dust mite extract shows activated expression in allergic individuals", Am J Respir Cell Mol Biol, 38(3), pp.293~299 (2008)
- Pulsawat P, "The house dust mite allergen Der p5 binds lipid ligands and stimulates airway epithelial cells through a TLR2-dependent pathway", Clin Exp Allergy, 49(3), pp.378~390 (2019)