기도상피세포에서 식물 에센셜 오일의 인터루킨-8 분비억제 효과
The Suppressive Effect of Essential Oils from Plants on Interleukin-8 from Airway Epithelial Cell
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Background: Neutrophil is an important inflammatory cell in severe asthma and associated with steroid-resistance in asthma therapy. In addition, interleukin (IL)-8 is a key chemokines recruiting and activating neutrophils. Methods: The aim of this study was to explore the therapeutic potential of aromatherapy using essential oils derived from plant extracts in asthma treatment. Essential oils from citron, pine tree, Chamaecyparis pisifera, and Chamaecyparis obtusa were extracted by water-steam distillation. The promoter activity and secretion of IL-8 were measured by using luciferase assay and enzyme-linked immunosorbent assay after pretreatment in tumor necrosis factor-α stimulated A549and human bronchial epithelial cells (BEAS-2B) cell lines with diluted essential oils. Results: IL-8 transcription was suppressed with 1 : 500 concentration of each essential oil (P＜0.05). IL-8 secretion in A549 cell was suppressed with 1 : 1,000 or higher concentrations of oils except from citron (P＜0.05). IL-8 secretion in BEAS-2B cells was decreased after exposure to 1 : 500concentration of pine tree, Chamaecyparis pisifera and 1 :2,000 or higher concentrations of Chamaecyparis obtusa (P ＜0.05). Conclusion: Essential oils from pine tree and Chamaecyparis obtusa may have a therapeutic potential for asthma by regulating neutrophil-mediated inflammation.
Abstract
Background: Neutrophil is an important inflammatory cell in severe asthma and associated with steroid-resistance in asthma therapy. In addition, interleukin (IL)-8 is a key chemokines recruiting and activating neutrophils. Methods: The aim of this study was to explore the therapeutic potential of aromatherapy using essential oils derived from plant extracts in asthma treatment. Essential oils from citron, pine tree, Chamaecyparis pisifera, and Chamaecyparis obtusa were extracted by water-steam distillation. The promoter activity and secretion of IL-8 were measured by using luciferase assay and enzyme-linked immunosorbent assay after pretreatment in tumor necrosis factor-α stimulated A549and human bronchial epithelial cells (BEAS-2B) cell lines with diluted essential oils. Results: IL-8 transcription was suppressed with 1 : 500 concentration of each essential oil (P＜0.05). IL-8 secretion in A549 cell was suppressed with 1 : 1,000 or higher concentrations of oils except from citron (P＜0.05). IL-8 secretion in BEAS-2B cells was decreased after exposure to 1 : 500concentration of pine tree, Chamaecyparis pisifera and 1 :2,000 or higher concentrations of Chamaecyparis obtusa (P ＜0.05). Conclusion: Essential oils from pine tree and Chamaecyparis obtusa may have a therapeutic potential for asthma by regulating neutrophil-mediated inflammation.
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