성선자극호르몬유리호르몬 길항제인 Antide가 수컷 생쥐의 유해감각예민도에 미치는 영향

Abstract

To investigate testosterone effect on nociception, tail flick latency(TFL, sec, 50±1。C) was measured before and after administration of antide(a gonadotropin releasing hormone antagonist) in male mice(Institute of Cancer Research, age of 7-8 weeks, weight 30.5-37.5 gm). Testosterone replacement effect on TFL was also examined in antide pre-treated male mice.Experiment 1: 30 male mice were randomized into 3 groups(N=10 each). Antide 100 ㎍/kg, 300 ㎍/kg or same volume(5.7 ml/kg) of 0.2% bovine serum albumin was administered intraperitoneally. TFL was measured before and at 3, 6, 9, 12 and 24 hours after injection.Experiment 2: 40 novice male mice were randomized into 2 groups(N=20 each) and both groups were pre-treated with antide 300 ㎍/kg i.p. 6 hours after antide injection, testosterone 1mg/kg or same volume(5.7 ml/kg) of sesame oil was administered i.p. TFL was measured before and 6 hours after antide pre-treatment and 30 min after testosterone/vehicle injection.