스트레스 반응에 대한 생물학적 표지자로서의 타액 알파 아밀라아제의 유용성
Salivary α-Amylase as a Biological Marker for Stress Reaction
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives：This study aimed at evaluating the changes of salivary α-amylase(sAA) reactivity to stress in the re ¬peated measurements to understand the correlation with state and trait anxiety. Methods：Thirty-six normal healthy volunteers carried out a stress-provoking experiment three times weekly. Su ¬bjects were asked to see 20 negative affective pictures to evoke negative emotion for 5 minutes. We collected 1 saliva samples before the stressor and 3 after, at 0 min post-stress and every 5 min for 10 min. Trait- and state-dependent psychological scales were measured before and during the experiment. Results：First, the levels of sAA were relatively consistent without significant differences throughout 3 repeated measurements. The reactivity of sAA also showed similar patterns between experiments before and after stress. Second, when comparing higher anxiety group(anxiety stress index≥10) with lower anxiety group(anxiety stress in¬dex<10), higher anxiety group showed lower sAA reactivity than lower anxiety group. This discrepancy was maintained in all repeated measurement. Third, the level of sAA was also related with state anxiety after stress. However, this pattern disappeared at third experiment. Conclusion：The basal level and reactivity of sAA were relatively stable over three repeated measurements accor ¬ding to the baseline anxiety sensitivity. They also showed the correlation with the state of anxiety until second trials. Therefore, these findings suggest that the sAA can reflect both state and trait of anxiety, and more importantly, display individual trait of anxiety stably even though the measurements are repeated.
Abstract
Objectives：This study aimed at evaluating the changes of salivary α-amylase(sAA) reactivity to stress in the re ¬peated measurements to understand the correlation with state and trait anxiety. Methods：Thirty-six normal healthy volunteers carried out a stress-provoking experiment three times weekly. Su ¬bjects were asked to see 20 negative affective pictures to evoke negative emotion for 5 minutes. We collected 1 saliva samples before the stressor and 3 after, at 0 min post-stress and every 5 min for 10 min. Trait- and state-dependent psychological scales were measured before and during the experiment. Results：First, the levels of sAA were relatively consistent without significant differences throughout 3 repeated measurements. The reactivity of sAA also showed similar patterns between experiments before and after stress. Second, when comparing higher anxiety group(anxiety stress index≥10) with lower anxiety group(anxiety stress in¬dex<10), higher anxiety group showed lower sAA reactivity than lower anxiety group. This discrepancy was maintained in all repeated measurement. Third, the level of sAA was also related with state anxiety after stress. However, this pattern disappeared at third experiment. Conclusion：The basal level and reactivity of sAA were relatively stable over three repeated measurements accor ¬ding to the baseline anxiety sensitivity. They also showed the correlation with the state of anxiety until second trials. Therefore, these findings suggest that the sAA can reflect both state and trait of anxiety, and more importantly, display individual trait of anxiety stably even though the measurements are repeated.
참고문헌 (41)
- McEwen BS, "Stress and the individual. Mechanismsleading to disease", Arch Intern Med, 153, pp.2093~2101 (1993)
- Cannon WB, "Bodily Changes in Pain, Hunger, Fear,and Rage" (1929), New York: Appleton
- Goldstein DS, "Encyclopedia of Stress. In: Fink G, editors. Sympathetic nervous system", pp.558~565 (2000), San Diego: Academic Press
- Kvetnansky R, "Encyclopedia of Stress. In: Fink G, editors. Adrenal medulla", pp.63~70 (2000), San Diego: Academic Press
- Ehlert U, "Salivary alpha amylase levels after yohimbine challenge in healthy men", J Clin Endocrinol Metab, 91, pp.5130~5133 (2006)
- Nater UM, "Sex differences in emotional and psychophysiological responses to musical stimuli", Int J Psychophysiol, 62, pp.300~308 (2006)
- Nater UM, "Stress-induced changes in human salivary alpha-amylase activity-associations with adrenergic activity", Psychoneuroendocrinology, 31, pp.49~58 (2006)
- Kirschbaum C, "Salivary cortisol in psychoneuroendocrine research: recent developments and applications", Psychoneuroendocrinology, 19, pp.313~333 (1994)
- Schwab KO, "Free epinephrine, norepinephrine and dopamine in saliva and plasma of healthy adults", Eur J Clin Chem Clin Biochem, 30, pp.541~544 (1992)
- Mitome M, "Salivary catecholamine assay for assessing anxiety in pediatric dental patients", J Clin Pediatr Dent, 21, pp.255~260 (1997)
- Okumura T, "Study of salivary catecholamines using fully automated column switching high-performance liquid chromatography", J Chromatogr B Biomed Sci Appl, 694, pp.305~316 (1997)
- Kennedy B, "Catecholamines in human saliva", Life Sci, 69, pp.87~99 (2001)
- Chatterton Jr. RT, "Salivary alpha-amylase as a measure of endogenous adrenergic activity", Clin Physiol, 16, pp.433~448 (1996)
- Baum BJ, "Principles of saliva secretion", Annals of the New York Academy of Sciences, 694, pp.17~23 (1993)
- Smith PM, "In: Edgar WM, O’Mullane D, editors. Mechanisms of secretion by salivary glands", pp.9~25 (1996), BDJ Books
- van Stegeren A, "Salivary alpha amylase as marker for adrenergic activity during stress: effect of betablockade", Psychoneuroendocrinology, 31, pp.137~141 (2006)
- Takai N, "Effect of psychological stress on the salivary cortisol and amylase levels in healthy young adults", Arch Oral Biol, 49, pp.963~968 (2004)
- Bosch JA, "Psychological stress as a determinant of protein levels and salivary-induced aggregation of Streptococcus gordonii in human whole saliva", Psychoso Med, 58, pp.374~382 (1996)
- Skosnik PD, "Modulation of attentional inhibition by norepinephrine and cortisol after psychological stress", Int J Psychophysiol, 36, pp.59~68 (2000)
- Foerster F, "The differentiation of individual-specific, stimulus-specific, and motivation-specific response patterns in activation processes: an inquiry investigating their stability and possible importance in psychophysiology", Biol Psychol, 17, pp.1~26 (1983)
- Marwitz M, "On the status of individual response specificity", Psychophysiol, 35, pp.1~15 (1998)
- Salmon P, "Anxiety, type A personality and endocrine responses to surgery", Br J Clin Psychol, 28, pp.279~280 (1989)
- Lehmann J, "Differences in mental task performance and slow potential shifts in subjects differing in cortisol level", Int J Psychophysiol, 13, pp.1~8 (1992)
- Seeman TE, "Self-esteem and neuroendocrine response to challenge: MacArthur studies of stressful aging", J Psychosom Res, 39, pp.69~84 (1995)
- Vedhara K, "An investigation into the relationship between salivary cortisol, stress, anxiety and depression", Biol Psychol, 62, pp.89~96 (2003)
- First MB, "Structured Clinical Interview for DSM-IV Axis I Disorders, Non- Patient Edition", pp.1995, New York: State Psychiatric Institute
- Beck AT, "Beck Depression Inventory(BDI)", Arch Gen Psychiatry, 4, pp.561~571 (1961)
- 이민규, "한국판 Beck 우울증 척도 표준화연구 II: 타당도 연구", 정신병리학, 4, pp.96~104 (1995)
- Beck AT, "An inventory for measuring clinical anxiety: Psychometric properties", J Consult Clin Psychol, 56, pp.893~897 (1988)
- 육성필, "한국판Beck Anxiety Inventory의 임상적 연구: 환자군과 비환자군의 비교", 한국심리학회지: 임상, 16, pp.185~197 (1997)
- Tylor S, "An Expanded Anxiety Sensitivity Index: Evidence for a Hierarchic Structurein a Clinical Sample", J Anx Dis, 12, pp.463~483 (1998)
- 김지혜, "한국어 확장판 불안 민감도 지수의 타당화 연구", 신경정신의학, 43(1), pp.54~61 (2004), 대한신경정신의학회
- Spielberger CD, "Manual for the state-trait anxiety inventory(STAI Form Y). Palo Alto" (1970), CA: Consulting Psychologists Press
- 김정택, "STAI의 한국표준화에 관한 연구", 최신 의학, 21, pp.69~75 (1978)
- Lang PJ, "International affective picture system(IAPS): Instruction manual and affective ratings", Technical Report A-6 The Center for Research in Psychophysiology University of Florida (2005)
- Wolf JM, "Chronic stress, salivary cortisol, and a-amylase in children with asthma and healthy children", Biol Psychol, 78, pp.20~28 (2008)
- Gordis EB, "Salivary alpha amylase-cortisol asymmetry in maltreated youth", Horm Beh, 53, pp.96~103 (2008)
- Nater UM, "Determinants of the diurnal course of salivary alpha-amylase", Psychoneuroendocrinology, 32, pp.392~401 (2007)
- Edgar WM, "Saliva and dental health. Clinical implications of saliva: report of a consensus meeting", Br Dent J, 169, pp.96~98 (1990)
- Fabian TK, "Hsp70 is present in human saliva", Med Sci Monit, 9, pp.62~65 (2003)
- Mackie DA, "Mastication and its influence on human salivary flow and alpha-amylase secretion", Physiol Behav, 47, pp.593~595 (1990)