Monocrotaline으로 유발된 흰쥐의 간독성에 대한 해독정기탕의 효과
Effect of Haedokjeongki-tang in Rat Liver after Monocrotaline Exposure
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
MCT 유발 간독성 흰쥐에 해독정기탕의 투여가 시간경과에 따른 간세포의 항산화효소 활성변화, 세포사멸 억제와 간의 조직병리학적 변화에 미치는 영향을 조사하였다. 대조군은 생리식염수를 투여하였으며, 실험군은 해독정기탕 0.9 mL을 6시간, 24시간 간격으로 경구투여 하였다. 간기능의 지표가 되는 GPT 효소 활성은 해독정기탕 투여한 6시간 군에서 대조군에 비해 감소하였다. 항산화 효소인 SOD, catalase 활성도의 변화에 있어서도 6시간 실험군에서 변화를 보여주었고 24시간 경과 후에는 대조군과 실험군의 통계적인 유의성은 없었다. 간의 조직병리학적 관찰 결과 대조군은 심한 출혈, 간세포의 팽창과 중심정맥 내피세포의 파괴 현상이 뚜렷이 나타났으며. 24시간 경과 후에는 핵질의 응축과 사립체의 팽대현상이 현저하였다. 반면 해독정기탕 투여후 6시간 실험군은 일부 세포 괴사와 세포사멸 보호 및 회복효과를 관찰하였다. 이상의 연구 결과로 보아 해독정기탕은 투여시간에 따라 간 기능회복 및 해독, 간세포의 항산화 활성변화, 세포사멸의 보호효과 약물로서 작용함이 사료된다.
Abstract
Monocrotaline(MCT) is a pyrrolizidine alkaloid(PA) plant toxin that produces hapatotoxicity in humans and animals. To test the hypothesis, we investigated the possible protective effects of Haedokjeongki-tang as an antioxidant against MCT-induced liver injury in rats. Cells with apoptotic morphology have been observed in the livers of animals exposed to PA and Haedokjeongki-tang. Whether apoptosis occurs in the livers of MCT-treated animals and whether it is required for full manifestation of pathological changes is not known, To determine this, rats were treated with 100 mg MCT/kg, and apoptosis was detected by transmission electron microscopy and TUNEL assay. MCT produced apoptosis in the liver by 6 h after treatment and increased by 24 h. Administration of Haedokjeongki-tang did affect liver structure and inhibit apopotosis in MCT-induced liver injury. Upon light and electron microscopic examination, we observed that Haedokjeongki-tang improved the morphological and histopathological changes of the liver caused by MCT-induced injury. MCT caused a time-dependent release of GOT and GPT, a marker of liver injury. Furthermore, we observed with respect to antioxidants status, catalase and superoxide dismutase activity tended to be higher in the MCT-treated rats than in the Haedokjeongki-tang administered rats. Our finding showed that Haedokjeongki-tang administration partially reduced liver injury after MCT exposure.
참고문헌 (23)
- "production and role in the kidney", pp.765~776 (1986)
- Beers RJ, "Spectrophotometric method for measuring the breakdown of H2O2 by catalase", pp.133~140 (1952)
- Brayan LC, "Modes of cell death in rat liver after monocrotalin exposure", 77, pp.172~182 (2004)
- Copple BL, "Anticoagulants prevent monocrotaline-induced hepatic parenchymal cell injury but not endothelial cell injury in the rat", 180, pp.186~196 (2002)
- Copple BL, "Liver inflammation during monocrotaline hepatotoxicity", 190, pp.155~169 (2003)
- Corcoran GB, "Buttyan R; Contemporary in toxicology molecular control point in toxicity", pp.169~181 (1994)
- Deng HL, "Anti-lipid peroxidative effect of ginsenoside Rb1 and Rg1" (1991)
- Gorden GJ, "Bax-mediated apoptosis in the livers of rat after hepatectomy in the retrorsine model of hepatocellular injury", 32, pp.312~320 (2000)
- Gujral JS, "Mode of cell death after acetaminophen overdose in mice", 67, pp.322~328 (2002)
- Hongo M, "Effects of nicorandil on monocrotaline-induced pulmonary arterial hypertension in rats", 46, pp.452~458 (2005)
- Huxtable RJ, "Human health implications of PA and herbs containing them In Toxicant of plant Origin 1", pp.41~86 (1989)
- Jaeschke H, "Activation of caspase 3-like proteases is essential for TNF-alpha induced hepatic parenchymal cell apoptosis and neutrophil-mediated necrosis in a murine endotoxin shock model", 160, pp.3480~3486 (1998)
- Janssen YMW, "Biology of disease cell and tissue responses to oxidative damage", pp.261~274 (1993)
- Kim SY, "The effect of betaine on the CCl4-induced hepatoxicity in rats", pp.538~543 (1993)
- Mattocks AR, "Estimation of metabolites of PA in animal tissues", pp.529~535 (1970)
- Meyrick BW, "Development of crotalaria pulmonary hypertention", pp.692~702 (1980)
- Reitman FA, "Studies on calcium transport during carbon tetrachloride mediated hepatotoxicity in mice", pp.4584~6 (1988)
- Schoental R, "Pathological changes in rats as a result of treatment with monocrotaline", pp.229~237 (1955)
- Schultz AE, "Chronic pulmonary hypertension: The monocrotaline model and involvement of the hemostatic system", 1, pp.271~346 (1998)
- Schumann J, "Importance of Kupffer cells for T-cell dependent liver injury in mice", 157, pp.1671~1683 (2000)
- Shi J, "Evidence of hepatocyte apoptosis in rat liver after the administration of carbon tetrachloride", 153, pp.515~525 (1998)
- Willson DW, "Mechanism and pathology of monocrotaline pulmonary toxicity", pp.307~325 (1992)
- Yacoub LK, "Apoptosis and bcl-2 protein expression in experimental alcoholic liver disease in the rat", 19, pp.854~859 (1995)