본문 바로가기
KERD
논문KCI 미등재

생물학분야에서 Cryo-electron Tomography 활용기법

Techniques for Cryo-electron Tomography in Biological Field

1

조회

0

다운로드

7

인용

0

참고문헌

키워드

주제분류자연과학일반

초록

In Biology, Studies Using Electron Microscopy for making Cell Structure to 3D reconstruction very fast development. Recently, by using Cryo fixation, we can see cell 3D structure without structural change, instead of using chemical fixation which can change cell structure. Before using this technology, we could understand cell structures only in 2D images. But now, through cryo-ET, 3D reconstruction of cell structure without artificial structure changes can be possible and this technology will give us many advantages in Drug delivery and Nanothechnology.

Abstract

In Biology, Studies Using Electron Microscopy for making Cell Structure to 3D reconstruction very fast development. Recently, by using Cryo fixation, we can see cell 3D structure without structural change, instead of using chemical fixation which can change cell structure. Before using this technology, we could understand cell structures only in 2D images. But now, through cryo-ET, 3D reconstruction of cell structure without artificial structure changes can be possible and this technology will give us many advantages in Drug delivery and Nanothechnology.

참고문헌 (14)

  1. Al-Amoudia A, "Cryo-electron microscopy of vitreous sections of native biological cells and tissues", J Struct Biol, 148, pp.131~135 (2004)
  2. Almgren M, "Cryo transmission electron microscopy of liposomes and related structures", Colloids Surf A Physicochem Eng Asp, 174, pp.3~21 (2000)
  3. Bohm J, "Toward detecting and identifying macromolecules in a cellular context: Template matching applied to electron tomograms", PNAS, 97, pp.14245~14250 (2000)
  4. Ding B, "Routine cryofixation of plant tissue by propane jet freezing for freeze substitution", J Electron Microsc Tech, 19, pp.107~117 (1991)
  5. Duman JG, "Three-dimensional reconstruction of cytoplasmic membrane networks in parietal cells", J Cell Sci, 115, pp.1251~1258 (2002)
  6. Hess MW, "Of plants and other pets:practical aspects of freezesubstitution and resin embedding", Microsc, 212, pp.44~52 (2003)
  7. Kellenberger E, "The response of biological macromolecules and supramolecular structures to physics of specimen cryopreparation. In Cryotechniques in biological electron microscopy", pp.35~63 (1987), Springer-Verlag
  8. Lee KE, "Cryo-elctron tomography", Mol Cell Biol news, 19, pp.26~35 (2007)
  9. Lucic V, "Structural studies by electron tomography: from cells to molecules", Annu Rev Biochem, 74, pp.833~865 (2005)
  10. McDonald K, "New developments and applications of high pressure freezing", Microsc Microanal, 11, pp.1092~1093 (2005)
  11. Monaghan P, "High-pressure freezing for immunocytochemistry", J Microsc, 192, pp.248~258 (1998)
  12. Nicastro D, "3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography", PNAS, 102, pp.15889~15894 (2005)
  13. Reipert S, "High-pressure freezing of epithelial cells on sapphire coverslips", J Microsc, 21, pp.81~85 (2004)
  14. Robards AW, "Low-temperature Methods for TEM and SEM. In Procedures in electron microscopy", pp.16~ (1997), John Wiley & Sons

관련 논문AI추천

이 간행물의 다른 논문 (8건)