Scanning Electron Microscopic Observation of Human Skin Replica
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초록
The skin is the largest organ of the integument system whose surface is closely related with many physiological and pathological conditions. Various methods are used to understand the structural and functional status of human skin. We would like to present usefulness of scanning electron microscopic (SEM)observation of skin replica and its significance of training module for a novice. The silicon replicas from several regions of the body (hand, finger,forearm, lip, and face) were casted by applying Exafine® mixture. The positive replicas were prepared by applying EPON 812 mixture on negative silicon replicas. Some of the negative silicon replicas were cut with a razor blade and surface profiles were observed. The negative and positive replicas were coated with platinum and were observed under the scanning electron microscope. We could investigate the detailed structures of the human skin surface without any physical damage to the subject. The positive replicas depicted real surface structure of the human skin vividly. The cross sectional view of the negative silicon replicas provided surface profile clearly. The scanning electron microscopic observation of the human skin replicas would be useful to study skin surface structures and to evaluate medical and esthetical applications.
Abstract
The skin is the largest organ of the integument system whose surface is closely related with many physiological and pathological conditions. Various methods are used to understand the structural and functional status of human skin. We would like to present usefulness of scanning electron microscopic (SEM)observation of skin replica and its significance of training module for a novice. The silicon replicas from several regions of the body (hand, finger,forearm, lip, and face) were casted by applying Exafine® mixture. The positive replicas were prepared by applying EPON 812 mixture on negative silicon replicas. Some of the negative silicon replicas were cut with a razor blade and surface profiles were observed. The negative and positive replicas were coated with platinum and were observed under the scanning electron microscope. We could investigate the detailed structures of the human skin surface without any physical damage to the subject. The positive replicas depicted real surface structure of the human skin vividly. The cross sectional view of the negative silicon replicas provided surface profile clearly. The scanning electron microscopic observation of the human skin replicas would be useful to study skin surface structures and to evaluate medical and esthetical applications.
참고문헌 (15)
- Choi HC, "Evaluation of skin furrows in the ageing process using an image analysis system", Kor J Dermatol, 35(2), pp.292~302 (1997)
- Dessy LA, "An objective assessment of botulinum toxin A effect on superficial skin texture", Ann Plast Surg, 58, pp.469~473 (2007)
- Fischer TW, "Direct and non-direct measurement techniques for analysis of skin surface topography", Skin Pharmacol Appl Physiol, 12, pp.1~11 (1999)
- Gilchrest BA, "A review of skin ageing and its medical therapy", Br J Dermatol, 135, pp.867~875 (1996)
- Grove GL, "Skin replica analysis of photodamaged skin after therapy with tretinoin emollient cream", J Am Acad Dermatol, 25, pp.231~237 (1991)
- Hortolà P, "Using digital anaglyphy to improve the relief effect of SEM micrographs of bloodstains", Micron, 40, pp.409~412 (2009)
- Junqueira LCU, "Carneiro J: Basic histology: text & atlas", pp.360~366 (2005), McGraw-Hill
- Kawada A, "Evaluation of anti-wrinkle effects of a novel cosmetic containing niacinamide", J Dermatology, 35, pp.637~642 (2008)
- 김지웅, "어도비포토샵 프로그램을 이용한 anaglyph 입체영상 제작법", 한국현미경학회지, 37(2), pp.143~146 (2007), 한국현미경학회
- Lagarde JM, "Topography and anisotropy of the skin surface with ageing", Skin Res Technol, 11, pp.110~119 (2005)
- Marks R, "Mechanical properties of the skin, in : Biochemistry and Physiology of the Skin", pp.1237~1254 (1983), Oxford University Press
- Park SY, "Quantitative evaluation of severity in psoriatic lesions using three-dimensional morphometry", Exp Dermatol, 13, pp.223~228 (2004)
- Rubino C, "A prospective study of anti-aging topical therapies using a quantitative method of assessment", Plast Reconstr Surg, 115, pp.1156~1162 (2005)
- Son SW, "Objective evaluation for severity of atopic dermatitis by morphologic study of skin surface contours", Skin Research Technology, 11, pp.272~280 (2005)
- Zou Y, "Age-dependent changes in skin surface assessed by a novel two-dimensional image analysis", Skin Res Technol, 15, pp.399~406 (2009)