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생쥐 근위세뇨관에서 납에 의한 신장 독성에 대한 스쿠알렌의 효과

Effects of Squalene on Renal Toxicity Induced by Lead Acetate in Proximal Tubules of the Mice

이유현|이준행|김종세
한국현미경학회 (Korean Society Of Microscopy)|2004.09|Applied Microscopy|제34권 제3호|pp. 185-197

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참고문헌

주제분류자연과학일반

초록

본 연구의 목적은 납중독에 의한 신장 기능 손상에 대한 스쿠알렌의 효과를 관찰하고자 하였다. ICR계 건강한 생쥐를 사용하여 납과 스쿠알렌을 복강 투여한 후, NO와 조직학적 변화를 관찰하였다. 실험군 설정은 다음과 같다. 실험군 1은 정상군, 실험군 2는 납 (30 ㎎/㎏)만 처치한 군, 실험군 3은 납을 처치하고 스쿠알렌 (180 ㎎/㎏)도 함께 처치한 군으로 각 실험군 당 생쥐 10마리를 사용하였다. 결과는 다음과 같다. 실험군 2의 경우, 미토콘드리아 신장, 사립체내막의 파괴, 소포체에서 리보소옴의 탈락이 24, 48 시간 동안 관찰되었고, 72 시간부터 서서히 회복 되는 것을 관찰할 수 있었다. 실험군 3의 경우 실험군 2에 비해 손상 정도가 덜 하였으며, 48 시간 이후부터 정상군과 유사한 소견을 보였다. NO의 경우 실험군 2에서는 NO 수치가 감소하였다. 하지만, 실험군 3의 경우 정상군 보다 NO가 증가하였을 뿐만 아니라 납에 의한 NO 수치 감소에 대해 회복 효과를 보였다. 위와 같은 결과로 보아, 스쿠알렌이 신장 근위세뇨관에 미치는 납 독성을 감소시키면서 빠른 회복에 효과가 있을 것으로 사료된다.

Abstract

To investigate the effect of the squalene against the lead toxicity and recovery of renal failure. Healthy male ICR mice were used for experiment. The activity of nitric oxide (NO) was observed after the intraperitoneal injection in mice. The ultrastructural changes of the kidney were observed after the intraperitoneal injection of lead acetate in mice. The experimental groups were divided into three groups. Group 1 was normal mouse. Group 2 was not treatment with squalene after intraperitoneal contamination of lead acetate (30 ㎎/㎏). And, Group 3 was injected squalene (180 ㎎/㎏) after intraperitoneal contamination of lead acetate. All groups were used to 10 mice. The results were as follow: In the case of the group 2, swelling of the outer membrane and destruction of the inner membrane (cristae) of the mitochondria, dripping of the ribosomes from the rough endoplasmic reticulum were happened at 24 hours and 48 hour. These were gradually reparied after 72 hours. In the case of the group 3, damages of the mitochondria and the rough endoplasmic reticulum were showed less than the group 2 at 24 hours. Especially, after 48 hours, these were almost same as the group 1.In the case of group 2, the level of NO was decreased. However, In the case of group 3, the level of NO was increased more than normal as well as repaired the decreased NO level by Pb (P<0.05). It was concluded that the squalene was the protective and recovery effects for the toxicity of the lead in the renal proximal tubules.

참고문헌 (41)

  1. "Subcellular distribution of lead in the rat", pp.705~712 (1971)
  2. "Fish oil versus olive oil", 336, pp.1313~1314 (1990)
  3. "Morphologic study of the effect of activated carbon treatment on the mouse kidney treated with lead" (1998)
  4. "Ultrastructural alternations produced in cockerels after mercuric chloride toxicity and subsequent interaction with an organophosphate insecticide", 24, pp.445~451 (1992)
  5. "Histological study on the effects of the green-tea in rat liver toxicated by lead", 30(2), pp.165~172 (2000)
  6. "Down-regulation of hepatic peripheral-type benzodiazepine receptors caused by acute lead intoxication", 293, pp.335~339 (1995)
  7. "The renal tubule in lead poisoning", 19, pp.71~77 (1968)
  8. "Nitric oxide selectively amplifies FGF-2-induced mitogenesis in primary rat aortic smooth muscle cells", pp.~1048 (1994)
  9. "role for L-arginine deiminase and imino nitrogen oxidation to nitrite", pp.473~235 476 (1987)
  10. "Effects of vitamin E and", or C on reactive oxygen species related lead toxicity in the rat sperm, 1998, pp.128, 169~179
  11. "Histopathological changes induced in rat tissues by oral intake of lead acetate", 41, pp.23~28 (1986)
  12. "Effects of squalene in the mouse kidney with contamination mercury", 30(4), pp.389~401 (2000)
  13. "Healing effects of squalene on the epifermis in burned mouse", pp.163~175 (1999)
  14. "Effects of squalene on mouse liver toxicity with cadmium", 30(2), pp.141~152 (2000)
  15. "Effects of chitosan on the rat nephrotoxicity induced by lead", 32(3), pp.205~211 (2002)
  16. "Induction of nitric oxide synthase and nitric oxide-mediated apoptosis in neuronal PC12 cells after stimulation with tumor necrosis factor-", lipopolysaccharide, 1998, pp.71, 88~94
  17. "Anemia of lead intoxification A role of for copper", pp.1779~1785 (1977)
  18. "Kinetic study of squenching reaction of singlet oxygen and scavenging reaction of free radical by squalene in n-butanol", 1256(1), pp.52~56 (1995)
  19. "Pathological study on the antidotal effects of D-penicillamine in lead poisoned dogs", 13(1), pp.25~32 (1997)
  20. "Lipopolysaccharide and ceramide use divergent signaling pathways to induce cell death in murine macrophages", 161, pp.2490~2500 (1998)
  21. "Measurement of squalene in human tissues and plasma", pp.38~45 (1976)
  22. "Determination and metabolism of dithiol chelating agents a major urinary excretion of metabolism of DMSA in the human increase the urinary excretion of lead in the rat", maiori(rmmm), pp.3 1221~2 1226 (1993)
  23. "Characterization studies on the 63 000 dalton 203 Pb binding component of rat kidney cytosol", pp.527~535 (1982)
  24. "Vascular endothelial cells synthesize nitric oxide from L-arginine", pp.109 664~142 333 666
  25. "Nitric oxide inhibits growth of glomerular mesangial cells role of the transcription factor EGR-1", 57, pp.70~82 (2000)
  26. "Localization of lead in the kidney and liver of rats treated in vivo with lead acetate ultrastructural studies on unstained sections", pp.221~234 (1994)
  27. "Purification and characterization of a human NO synthase", pp.1372~1377 (1991)
  28. "Formation and release of nitric oxide from human neutrophils and HL-60 cells induced by a chemotactic peptide", pp.357~360 (1989)
  29. "The influence of iron deficiency on tissue content and toxicity of ingested lead in the rat", pp.128~136 (1972)
  30. "Stable isotope labeling of lead compartments in rats with ultralow lead concentration", 57, pp.190~207 (1992)
  31. "Pathogenesis of acute renal failure", pp.277~333 (1983), Int Rev Exp 24
  32. "A study on the effect of garlic and 2 3-Dimercaptosuccinic acid on the cadmium poisoning of pregnant rat", pp.237~245 (1986)
  33. "Radioprotection of mice by dietrary squalene", 28, pp.555~559 (1993)
  34. "A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells", pp.1543~1555 (1989)
  35. "Biochemical changes in the kidneys after perinatal intoxication with lead and", or cadmium and their antagonistic effects when coadministered, 2003, 1~6
  36. "and cholesterol levels in human organs", pp.35~40 (1980)
  37. "Nitric oxide generation by renal proximal tubules role of nitric oxide in the cytotoxicity of lipid A", 279, pp.91~96 (1996)
  38. "Spectrophotometric quantitation of anchorage-dependent cell numbers using the bicinchoninic acid protein assay reagent"
  39. "Antitumor activity of squalene-treated cell wall skeleton of Nocardia rubra in mice", 69, pp.619~629 (1978)
  40. "Chitosan increase the release of renal dipeptidase from pocrine renal proximal tubule cells", 7, pp.309~315 (2003)
  41. "Ultrastructural influence of heavy metallic agents on the proximal convoluted tubule of mice", 1(2), pp.719~733 (1995)

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