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납으로 유발된 생쥐 간독성에 대한 활성탄의 보호효과

Protective effects of activated charcoal on the mouse hepatotoxicity induced by lead

노영복|정민주
한국현미경학회 (Korean Society Of Microscopy)|2006.12|Applied Microscopy|제36권 제4호|pp. 235-250

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참고문헌

주제분류자연과학일반

초록

본 연구는 급성 납 독성에 대한 활성탄의 방어효과를 알아보기 위하여 시도되었다. 30g 내외의 생쥐를 대상으로 대조군, 납(60㎎/㎏) 투여군, 납 투여후 활성탄(40㎎/㎏) 투여군으로 구분하여 구강투여 한 후 간장, 신장 손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase (AST), alanine aminotransferase (ALT) 그리고 glucose (Glu)의 수치는 활성탄 투여군이 납 투여군보다 감소되었다. 전자현미경적 관찰 결과, 간장에서 납 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 활성탄 투여군은 핵이 대조군과 같은 형태가 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상과 같은 결과, 활성탄이 납 중독된 생쥐에서 납을 흡착시킴으로서 손상된 간장에 보호 효과가 있는 것으로 사료된다.

Abstract

A protective effect of activated charcoal against the acute lead poisoning of kidney was studied in mice. Mice approximately 30 gm in weight were grouped into the control, lead acetate-treated, and activated charcoal-treated after lead acetate groups. Lead acetate (60mg/kg) and activated charcoal (40mg/kg) were delivered orally. Serum AST, ALT and glucose were measured and the ultrastructural alteration of liver was examined by electron microscopy. Activated charcoal decreased the increase of serum AST, ALT and glucose levels induced by lead.Lead acetate-treated hepatic cells characterized by irregular nuclei, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with activated charcoal were similar to those of the control group.In conclusion, activated charcoal may protect the lead-induced toxicity on liver.

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