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재생중인 흰쥐 간의 메탈로사이오닌에 대한 면역-금 표지 및 발현에 관한 연구

Immunogold Labellings and Expression of Metallothionein in Regenerating Rat Liver

이종화|김완종|이미영|김호진|안영모|오승한|신길상
한국현미경학회 (Korean Society Of Microscopy)|2005.03|Applied Microscopy|제35권 제1호|pp. 15-22

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참고문헌

키워드

RatImmunocytochemistryMetallothioneinPartial hepatectomy
주제분류자연과학일반

초록

Metallothionein(MT) is a low molecular weight, cysteine-rich, metal-binding, and non-enzymatic protein.regenerating rat liver. In partial hepatectomized rats, MT mRNA was detected as early as 1 hr and reached amaximal level by 8 hr after the operation. Thereafter, this level decreased gradually until 24hr, and it becamesimilar to that of sham control. Meanwhile, time course of MT immunoreactivity using immunogold-labellinghr after partial hepatectomy. Ultrastructurally, immunogold particles indicating the presence of MT weredistributed in both the cytoplasm and the nucleus of the rat hepatocytes, particularly the particles weredistributed at rough endoplasmic reticulum and nucleolus and did not seem to adhere to mitochondria orlysosomes in proliferating hepatocytes. Briefly, high level of MT mRNA expression and the intensesuggest that MT possibly involves hepatocyte proliferation via the storage or the supply of various metal ionsin the regenerating rat liver.

Abstract

Metallothionein(MT) is a low molecular weight, cysteine-rich, metal-binding, and non-enzymatic protein.regenerating rat liver. In partial hepatectomized rats, MT mRNA was detected as early as 1 hr and reached amaximal level by 8 hr after the operation. Thereafter, this level decreased gradually until 24hr, and it becamesimilar to that of sham control. Meanwhile, time course of MT immunoreactivity using immunogold-labellinghr after partial hepatectomy. Ultrastructurally, immunogold particles indicating the presence of MT weredistributed in both the cytoplasm and the nucleus of the rat hepatocytes, particularly the particles weredistributed at rough endoplasmic reticulum and nucleolus and did not seem to adhere to mitochondria orlysosomes in proliferating hepatocytes. Briefly, high level of MT mRNA expression and the intensesuggest that MT possibly involves hepatocyte proliferation via the storage or the supply of various metal ionsin the regenerating rat liver.

참고문헌 (24)

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관련 논문AI추천

흰쥐 부분 간 절제 후 재생 중인 간 조직의 구조와 metallothionein 분포김완종, 문승훈, 신길상, 이용문, 정진주한국현미경학회 · Applied Microscopy2008 · 제38권 제3호발생중인 흰쥐 간에서의 Metallothionein 발현에 관한 면역세포화학적 연구김완종, 신길상, 안영모, 오승한한국현미경학회 · Applied Microscopy2004 · 제34권 제3호위암 조직내 Metallothionein의 면역 세포화학적 연구김완종, 신길상, 양승하한국현미경학회 · Applied Microscopy2002 · 제32권 제4호Modified Neo-Timm’s sulphide-silver 방법 에 의한 조직내 아연의 검출정주연, 제갈승주대한임상검사과학회 · 대한임상검사과학회지1996 · 제28권 제1호내독소 투여에 의한 Kupffer 세포의 미세형태학적 해독반응이태숙, 최원희, 최준혁영남대학교 의과대학 · Journal of Yeungnam Medical Science1993 · 제10권 제2호재생 중인 흰쥐 간의 형태학적 변화 및 PCNA 발현에 미치는 rrhGM-CSF의 영향김완종, 김지현, 윤광호, 이영준, 정진주, 한규범, 허시현한국현미경학회 · Applied Microscopy2010 · 제40권 제2호

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