음용수 불소농도에 따른 흰쥐태아 상아모세포의 미세구조적 변화
Dose-dependent Ultrastructural changes of the Odontoblasts in the Neonatal Rat after Ingestion of the Fluoride to the Pregnant Rat
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음용불소가 흰쥐태아의 상아모세포에 간접적으로 미치는 영향과 농도를 확인하기 위해 임신한 흰쥐에 100,200, 300 ppm의 불소가 함유된 음용수를 임신기간 동안투여하였다. 대조군과 비교하였을 때, 농도에 의존적으로상아모세포의 미세구조적 변화를 관찰 할 수 있었다: 조면소포체로 부터 리보솜의 탈락과 붕괴, 미토콘드리아의팽창, 그리고 조면소포체와 미토콘드리아의 변성. 음용 불소의 농도에 따른 미세구조적인 변화로부터 100, 200ppm에서는 가역적인 그리고 300 ppm에서는 비가역적인손상이 유도되는 것을 확인하였다. 또한, 이러한 결과로미루어 200 ppm 미만의 농도가 발생중인 흰쥐태아에 영향을 주지 않는 것을 알 수 있다.Jeong MJ et al. : Ultrastructural Changes of the Odontoblast by Fluoride 21numerous endoplasmic reticulum and secretory vesicles of the low electron density. Scale bar= 2.3m. 4. The proximalportion of the odontoblast with a basally displaced nucleus (N) and associated endoplasmic reticulum (arrows), Golgiapparatus (G) and mitochondria (M). The Golgi region occupies the center of the cells and is surrounded by profiles of therough endoplasmic reticulum. Adjacent odontoblasts are tightly attached together by numerous adherence junctions. Scalebar= 0.3m.vacuoles appeared to be swollen and disrupted mitochondria (M). In the same cells there are a disintegration anddegranulation of the rough-surfaced endoplasmic reticulum(arrows) and of the poly-ribosomes. Respectively, Scale bar= 2.3m, Scale bar= 0.8m.22 Korean J. Electron Microscopy Vol. 36, No. 1, 200611 22EDPDOB33 44Jeong MJ et al. : Ultrastructural Changes of the Odontoblast by Fluoride 232233 44111DPDOBE
Abstract
The pregnant rats were given a drinking water administration of the sodium fluoride and normal saline forcontrol animals. The sodium fluoride produced cellular changes of odontoblast with consistent response.Compare to control group, the odontoblasts that were administrated by sodium fluoride, showed significantlyultrastructural differences including large number of free ribosomes and swelled mitochondria in dose-dependent manner(300ppm).From fine structural and morphological investigations of the changes in odontoblast, there were threedistinctive structural changes: (1) destruction of the endoplasmic reticulum, (2) swelling of the mitochondria,and (3) severe cellular derangement of the endoplasmic reticulum and mitochondria. From this consecutivestructural change, we observed that sodium fluoride temporarily affects the cell organelles in odontoblasts(100, 200ppm), suggesting it is important that optimal concentration of the sodium fluoride in developing fetusof the rat.