산왕거미(Araneus ventricosus) 혈구의 미세구조
The fine structure of spider (Araneus ventricosus) hemocytes
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초록
산왕거미 (Araneus ventricosus) 혈구의 유형을 세포화학적 및 percoll 밀도구배를 이용한 물리화학적 특성에 따라 구분하고, 각각의 미세구조를 고배율의 전자현미경으로 비교 관찰하였다. 혈구는 세포질에 함유된 과립의 유무에 따라 과립혈구와 무과립혈구로 구분되었으며, 과립혈구는 과립의 특성에 따라 다시 산호성 과립혈구와 염기호성 과립혈구, 그리고 헤모시아닌 과립을 함유한 혈색소혈구의 세 가지 유형으로 세분되었다. 산호성 과립혈구는 전체 혈구유형중 약 5% 정도를 차지하였으며, 염기호성 과립혈구는 산호성 과립혈구에 비해 과립의 크기가 크고 구형이었다. 한편 무과립혈구는 혈구중 가장 크기가 작은 투명혈구와 심관의 내강에 주로 분포하는 편도혈구, 그리고 탈피 시에만 출현하는 대형의 탈피혈구로 다시 세분되었다.
Abstract
Hemocytes of the spider Araneus ventricosus were investigated with histochemistry, density analysis of percoll gradient, and fine structural examinations using transmission electron microscope. The hemocytes of this spider were classified into two major groups: granulocytes and non-granulocytes. The granular hemocytes were subdivided into three subtypes according to their histochemical properties which are eosinophilic granulocytes (EGs), basophilic granulocytes (BGs) and cyanocytes. The EGs, which have small granules within the cytoplasm comprise about 5 % of the total hemocytes. However the granules of BGs are larger than those of EGs. The cyanocytes were characterized to contain hemocyanin granules in their cytoplasm. On the other hand, the non-granulocytes were divided into three subtypes: hyaline leucocytes, oenocytoids, and molting hemocytes. The hyaline leucocytes are the most abundant and the smallest hemocyte type in this spider. The oenocytoids that have 10-15 ㎛ in diameter are mostly found at the marginal region of the myocardium in the heart tube. The molting hemocytes, which only appeared during the molting period, contains plenty of glycogen particles in their cytoplasm.
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