참굴, Crassostrea gigas, haemocytes의 형태 및 면역학적 특징
Morphological and immunological characterizaiton of the haemocytes of the oyster, Crassostrea gigas
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패류의 방어시스템에서 순환 haemocytes는 중요하다. 참굴, Crassostrea gigas haemocytes의 형태적 특징을 이해하기 위해서 광학현미경적 특징과 전자현미경적 특징을 관찰하였다. 그 결과 4종류의 haemoctyes type을 확인하였다: type Ⅰ small hyalinocytes, type Ⅱ large hyalinocytes, type Ⅲ large granulocytes, type Ⅳ small granulocytes. 또한, 면역세포학적 방법으로 haemocytes의 alkaline phosphatase (ALP), acid phosphatase (ACP), peroxidase (POD), α-naphthyl acetate esterase, β-glucuronidase, PAS, sudan black B와 oil red O의 활성을 조사하였다. 그 결과, 조사한 모든 효소의 활성이 granulocytes에서 hyalinocytes에 비하여 높게 나타났다.Haemoctyes와 Vibrio FKC를 incubation 후 식균지수와 식균율을 조사하였다. 그 결과, 식균지수와 식균율은 배양 15분째부터 증가하여 120분까지 높게 나타났다. 또한, ALP, ACP, α-naphthyl acetate esterase와 β-glcuronidase의 효소활성도 대조구에 비하여 높게 나타나 이와 같은 효소는 참굴의 식작용에 중요한 역할을 하는 것으로 생각된다.
Abstract
The internal defense system of mollusks consists of circulating haemocytes. In order to understand the morphological characterization of haemocytes, light and electron microscopy were carried out in oyster, Crassostera gigas. Four types of haemocytes were recognized: type Ⅰ small hyalinocytes, type Ⅱ large hyalinocytes, type Ⅲ large granulocytes and type Ⅳ small granulocytes. Additionally, the activities of alkaline phosphatase (ALP), acid phosphatase (ACP), peroxidase (POD), α-naphthyl acetate esterase, β-glucuronidase, PAS, sudan black B and oil red O in haemocytes were analysed by immunocytochemical methods. The results indicate that enzymatic activities were abundant and more active in granulocytes than in hyalinocytes. After incubation with haemoctyes and Vibrio FKC, phagocytic index and percentage of phagocytic cell were and shown to be increased from 15 to 120 min. In addition, the enzymatic activities were higher than those of controls: ALP, ACP, α-naphthyl acetate esterase and β-glcuronidase, indicating that these enzymes can be related with phagocytosis in oyster.
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