흰넓적다리 붉은쥐(Apodemus speciosus peninsulae)의 세정관 상피주기
Seminiferous Epithelium Cycle of Apodemus speciosus peninsulae
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본 연구는 흰넓적다리 붉은쥐(Apodemus speciosus peninsulae)의 세정관 상피주기와 정자세포의 발달 단계를 광학 현미경을 사용하여 조사하였다. 세정관 상피주기는 정모세포의 분화단계와 정자세포의 발달단계를 기초로 하여 9 stages로 나누었다. Ad형 정원세포의 경우 모든 단계에서 관찰되었다. Ap형의 정원세포는 Ⅰ 단계, In형의 정원세포는 Ⅱ 와 Ⅲ 단계, B형의 정원세포는 Ⅳ 단계에서 관찰되었다. 제 1 감수분열의 전기에 있어서, 세사기는 Ⅴ와 Ⅵ 단계, 접합기는 Ⅰ 단계에서 부터 Ⅸ 단계까지, 후사기는 Ⅲ와 Ⅶ 단계, 복사기는 Ⅷ 단계에서 각각 관찰되었으며, 제 2정모세포의 경우는 Ⅸ 단계에서 나타났다. 정자세포의 발달단계는 정자세포의 머리 형태, 핵과 첨체의 발달, 세포질의 형태 변화를 기초로 하여 12 steps로 구분되어졌다. 본 연구의 결과 흰넓적다리 붉은쥐(A. s. peninsulae)는 정원세포 및 정모세포 출현시기와 정자발달 단계를 비교해 볼 때 근연종인 등줄쥐(A. agrarius coreae)와 매우 유사한 결과를 나타내었다. 본 연구에서 같은 유형의 세포가 출현하는 시기와 정자형성에 따른 세대 수는 종의 특성에 의한 것이라 생각되며, 이것은 종을 분류하는데 유용한 형질이라 여겨진다.
Abstract
The cycle of the seminiferous epithelium and development of spermatids of Apodemus speciosus peninsulae were observed using a light microscope. On the basis of developing spermatocyte and spermatid, the cycle of the seminiferous epithelium was divided into 9 stages. Type Ad spermatogonia were appeared in all stages (Ⅰ- Ⅸ). The Ap, In, and B types of spermatogonia were appeared from stage Ⅰ, Ⅱ and Ⅲ, and Ⅳ, respectively. In prophase of first meiosis, the leptotene spermatocytes appeared from stage Ⅴ and Ⅵ, zygotene spermatocytes from stages Ⅰ, Ⅱ, Ⅶ, Ⅷ, and Ⅸ, pachytene spermatocytes from stage Ⅲ to Ⅶ, diplotene spermatocytes in the stage Ⅷ, and secondary spermatocytes in stage Ⅸ. On the basis of morphology of spermatid head, developing of nuclear and acrosome and the morphological change of cytoplasm, the developing of spermatids was divided into 12 steps. Considering all the results, A. s. peninsulae displayed very similar result with A. agrarius coreae that is allied species when compare correct characters developing of spermatids with spermatogonia and appearance time of the spermatocyte. Appearance time of the same cell and number of spermatogonial generation was thought that characters of the species, and information may be useful in identifying the species.
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