Downstream Genes Regulated by Bcl2l10 RNAi in the Mouse Oocytes
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Previously, we have shown that Bcl2l10 as a member of Bcl-2 family, key regulators of the apoptotic process, is dominantly expressed in oocytes of ovary but several member of the Bcl-2 family are not expressed in oocytes. Recent our studies had been processed about roles and regulatory mechanisms of Bcl2l10 in oocytes. Microinjection of Bcl2l10 RNAi into the cytoplasm of germinal vesicle oocytes resulted in metaphase I (MI) arrest and exhibited abnormalities in their spindles and chromosome configurations (Yoon et al., 2009). The present study was conducted to elucidate the downstream genes regulated by Bcl2l10 and signaling networks in Bcl2l10 RNAi microinjected oocytes by using microarray analysis. Surprisingly, we found that a large proportion of genes regulated by Bcl2l10 RNAi were involved in the cell cycle and actin skeletal system regulation as important upstream genes of Bcl2l10. Among the transcripts with highly significant fold change of genes changed by more than 2-fold, Tpx2 and Cep192 are respectively 16.1- and 8.2-fold down regulated by Bcl2l10 RNAi. Tpx2 and Cep192 are known as cofactors that control Aurora A kinase activity and localization. Therefore, Bcl2l10 may have important roles during oocyte meiosis as functional upstream regulator of Tpx2 and Cep192.
Abstract
Previously, we have shown that Bcl2l10 as a member of Bcl-2 family, key regulators of the apoptotic process, is dominantly expressed in oocytes of ovary but several member of the Bcl-2 family are not expressed in oocytes. Recent our studies had been processed about roles and regulatory mechanisms of Bcl2l10 in oocytes. Microinjection of Bcl2l10 RNAi into the cytoplasm of germinal vesicle oocytes resulted in metaphase I (MI) arrest and exhibited abnormalities in their spindles and chromosome configurations (Yoon et al., 2009). The present study was conducted to elucidate the downstream genes regulated by Bcl2l10 and signaling networks in Bcl2l10 RNAi microinjected oocytes by using microarray analysis. Surprisingly, we found that a large proportion of genes regulated by Bcl2l10 RNAi were involved in the cell cycle and actin skeletal system regulation as important upstream genes of Bcl2l10. Among the transcripts with highly significant fold change of genes changed by more than 2-fold, Tpx2 and Cep192 are respectively 16.1- and 8.2-fold down regulated by Bcl2l10 RNAi. Tpx2 and Cep192 are known as cofactors that control Aurora A kinase activity and localization. Therefore, Bcl2l10 may have important roles during oocyte meiosis as functional upstream regulator of Tpx2 and Cep192.
참고문헌 (16)
- Adams JM, "The Bcl-2 protein family: arbiters of cell survival", Science, 281, pp.1322~1326 (1998)
- Barr AR, "Aurora-A: the maker and breaker of spindle poles", J Cell Sci, 120, pp.2987~2996 (2007)
- Bayliss R, "Structural basis of Aurora-A activation by TPX2 at the mitotic spindle", Mol Cell, 12, pp.851~862 (2003)
- Inohara N, "Diva, a Bcl-2 homologue that binds directly to Apaf-1 and induces BH3-independent cell death", J Biol Chem, 273, pp.32479~32486 (1998)
- Joukov V, "Aurora kinases and spindle assembly: variations on a common theme", Cell Cycle, 10, pp.895~903 (2011)
- Kim JH, "HIP1R interacts with a member of Bcl-2 family, BCL2L10, and induces BAK- dependent cell death", Cell Physiol Biochem, 23, pp.43~52 (2009)
- Kufer TA, "Human TPX2 is required for targeting Aurora-A kinase to the spindle", J Cell Biol, 158, pp.617~623 (2002)
- Lu PJ, "Bcl-2 overexpression inhibits cell death and promotes the morphogenesis, but not tumorigenesis of human mammary epithelial cells", J Cell Biol, 129, pp.1363~1378 (1995)
- Naumann U, "Diva", Boo is a negative regulator of cell death in human glioma cells, 2001, pp.505, 23~26
- Ozlu N, "An essential function of the C. elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles", Dev Cell, 9, pp.237~248 (2005)
- Song Q, "Boo, a novel negative regulator of cell death, interacts with Apaf-1", EMBO J, 18, pp.167~178 (1999)
- Tsai MY, "A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly", Nat Cell Biol, 5, pp.242~248 (2003)
- Wu MY, "Deficiency of Rbbp1", Arid4a and Rbbp1l1, AS domain, pp.Genes Dev, 2006[DOI]
- Xu X, "Two TPX2- dependent swithches control the activity of Aurora A", PLoS One, 6, pp.16757~ (2011)
- 이경아, "Mass Spectrometry를 이용한 난자 특이적인 Diva와 상호작용하는 단백질의 동정", Clinical and Experimental Reproductive Medicine, 33(3), pp.189~198 (2006), 대한생식의학회
- Yoon SJ, "Role of Bcl2-like 10 (Bcl2l10) in Regulating Mouse Oocyte Maturation", BIOLOGY OF REPRODUCTION, 81, pp.497~506 (2009), SOC STUDY REPRODUCTION